CAJ | 학술논문

水扎蛋白能介导水分的跨膜运输,在植物的生长发育过程中起重要作用.对RWC3启动子-GUS转基因水稻的组织化学染色表明,水稻(Oryza sativa L.)水孔蛋白RWC3可能在包括营养和生殖器官在内的各部位中广泛表达.同是发现,赤霉素(GA)能提高转基因植物的愈伤组织、悬俘细胞和叶片中的GUS活必性,而GA合成的抑制ancymidol降低了GUS活性.进一步研究发现,蔗糖能抑制GA对GUS活性的提高,说明GA和蔗糖在对RWC3表达调控的信号传递过程中可能存在着相互作用.
수찰단백능개도수분적과막운수,재식물적생장발육과정중기중요작용.대RWC3계동자-GUS전기인수도적조직화학염색표명,수도(Oryza sativa L.)수공단백RWC3가능재포괄영양화생식기관재내적각부위중엄범표체.동시발현,적매소(GA)능제고전기인식물적유상조직、현부세포화협편중적GUS활필성,이GA합성적억제ancymidol강저료GUS활성.진일보연구발현,자당능억제GA대GUS활성적제고,설명GA화자당재대RWC3표체조공적신호전체과정중가능존재착상호작용.
Water channel proteins facilitate water flux across cell membranes and play important roles in plant growth and development. By GUS histochemical assay in RWC3 promoter-GUStransgenic rice (Oryza sativa L. cv. Shenxiangjin 4), one of the members of water channel proteins in rice, RWC3, was found to distribute widely in variety of organs, from vegetative and reproductive organs. Further studies showed that gibberellin (GA) enhanced the GUS activity in the transgenic calli, suspension cells and leaves,whereas ancymidol (anc), an inhibitor of GA synthesis, reduced the GUS activity. Sucrose was found to inhibit the effects induced by addition of GA, suggesting a possible cross-talk between GA and sucrose signaling on regulation of the RWC3 gene expression.