CAJ | 학술논문

用RT-PCR技术从小立碗藓中(Physcomitrella patens)克隆了核编码的MinE基因,命名为PpMinE,并克隆了该基因的基因组DNA.序列比对显示该基因编码的蛋白质与真细菌和绿藻叶绿体编码的MinE蛋白具有较高的相似性.pMinE-EGFP融合蛋白在烟草中的瞬时表达证明该蛋白定位于叶绿体内.在大肠杆菌中过量表达PpMinE导致细胞不正常分裂,产生无染色体的小细胞,这表明MinE的功能在进化上是保守的.在系统发育树中,PpMinE和高等陆生植物有较近的亲缘关系.在已知的陆生植物的叶绿体基因组中没有找到MinE的同源蛋白,这暗示在进化过程中MinE从叶绿体到细胞核的水平转移可能发生在陆生植物发生以前.
용RT-PCR기술종소립완선중(Physcomitrella patens)극륭료핵편마적MinE기인,명명위PpMinE,병극륭료해기인적기인조DNA.서렬비대현시해기인편마적단백질여진세균화록조협록체편마적MinE단백구유교고적상사성.pMinE-EGFP융합단백재연초중적순시표체증명해단백정위우협록체내.재대장간균중과량표체PpMinE도치세포불정상분렬,산생무염색체적소세포,저표명MinE적공능재진화상시보수적.재계통발육수중,PpMinE화고등륙생식물유교근적친연관계.재이지적륙생식물적협록체기인조중몰유조도MinE적동원단백,저암시재진화과정중MinE종협록체도세포핵적수평전이가능발생재륙생식물발생이전.
A nucleus-encoded MinE gene, designated PpMinE, from Physcomitrella patens was identified using RT-PCR. The presence of both N- and C-terminal extensions in PpMinE protein suggested its cyanobacterial origin. The transient expression of PpMinE using green fluorescent protein fusion in tobacco (Nicotiana tabacum L.) indicated that the PpMinE was a chloroplast-targeted protein. Overexpression of PpMinE in Escherichia coli caused division site misplacement and minicell formation, suggesting evolutionary functional conservation of MinE during plant phylogenesis. According to the phylogenetic tree, PpMinE protein has a close relationship with the highland plants, which suggests that the transfer events of MinE gene from plastid to nucleus might have occurred before the origin of the land plants.