农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
6期
59-63
,共5页
苏友禄%冯娟%孙秀秀%郭志勋%闫云锋%黄剑南
囌友祿%馮娟%孫秀秀%郭誌勛%閆雲鋒%黃劍南
소우록%풍연%손수수%곽지훈%염운봉%황검남
Epinephelus akaara%NNV%MCP%Expression conditions
[Objective] To optimize the prokaryotic expression of MCP gene of red-spotted grouper nervous necrosis virus. [Method] The MCP gene was amplified from red-spotted grouper nervous necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21(DE3)plysS to express proteins with induction in different media, at different pH, or at different temperatures. [Result] The expression level of recombinant bacteria reached a peak with induction under the following condition: SOB or LB medium, pH 7.0, 37 ℃ while the fusion protein was about 44.5 kD in molecular weight. [Conclusion] This study provided a basis for the development of RGNNV-MCP vaccine.