中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2009年
7期
416-418
,共3页
硫酸卷曲霉素%组分分析%高效毛细管电泳法%液质联用
硫痠捲麯黴素%組分分析%高效毛細管電泳法%液質聯用
류산권곡매소%조분분석%고효모세관전영법%액질련용
Capreomycin sulfate%Component%HPCE%LC-MS
目的 建立分离硫酸卷曲霉素组份的高效毛细管电泳法(HPCE)和液质联用(LC-MS),定性定量分析各组分.方法 通过环糊精的种类及浓度、缓冲液的pH及浓度、温度及电压的优化,选择最佳的HPCE分离条件,并建立适合于LC-MS的分析条件.结果 最佳的HPCE缓冲液为30mmol/L磷酸盐缓冲液(pH 2.5,内含8 mmol/L羧甲基-β-环糊精);检测波长为214nm;电压为30kV;温度为30℃,基线分离了卷曲霉素4个组分;采用XBP-C18色谱柱,电喷雾ESI为电离源,分析检测了卷曲霉素的各组分.结论 建立的HPCE法和LC-MS法可用于为该产品的质量控制提供了可靠的分析方法 .
目的 建立分離硫痠捲麯黴素組份的高效毛細管電泳法(HPCE)和液質聯用(LC-MS),定性定量分析各組分.方法 通過環糊精的種類及濃度、緩遲液的pH及濃度、溫度及電壓的優化,選擇最佳的HPCE分離條件,併建立適閤于LC-MS的分析條件.結果 最佳的HPCE緩遲液為30mmol/L燐痠鹽緩遲液(pH 2.5,內含8 mmol/L羧甲基-β-環糊精);檢測波長為214nm;電壓為30kV;溫度為30℃,基線分離瞭捲麯黴素4箇組分;採用XBP-C18色譜柱,電噴霧ESI為電離源,分析檢測瞭捲麯黴素的各組分.結論 建立的HPCE法和LC-MS法可用于為該產品的質量控製提供瞭可靠的分析方法 .
목적 건립분리류산권곡매소조빈적고효모세관전영법(HPCE)화액질련용(LC-MS),정성정량분석각조분.방법 통과배호정적충류급농도、완충액적pH급농도、온도급전압적우화,선택최가적HPCE분리조건,병건립괄합우LC-MS적분석조건.결과 최가적HPCE완충액위30mmol/L린산염완충액(pH 2.5,내함8 mmol/L최갑기-β-배호정);검측파장위214nm;전압위30kV;온도위30℃,기선분리료권곡매소4개조분;채용XBP-C18색보주,전분무ESI위전리원,분석검측료권곡매소적각조분.결론 건립적HPCE법화LC-MS법가용우위해산품적질량공제제공료가고적분석방법 .
Objective Qualitatively and quantitatively analyses were employed to establish HPCE and LC-MS methods for the separation of each component of capreomycin sulfate. Methods By optimizing factors which affect separation, the kinds and concentration of cyclodextrin, the pH value and concentration of buffer, the voltage and temperature, the optimum conditions for separation were selected. By choosing chromatography condition, LC-MS methode was established. Results Using phosphoric buffer containing carboxymethyl-β-cyclodextrin, the base-line separation of each component was achieved. By using XBP-C18 column, electrospray ionization and positive ion monitoring, components of capreomycin were analysed. Conclusion The new established HPCE and LC-MS methods were useful to quality control of capreomycin sulfate.
