CAJ | 학술논문

以克氏原螯虾DNA为材料,对AFLP分析中的基因组酶切时间、选择性扩增中预扩增产物稀释倍数、Mg~(2+)浓度等3个因素进行了比较.结果表明,基因组用EcoR I和MseI双酶切5h,选择性扩增的PCR反应体系中Mg~(2+)1.5 mmol/L是进行克氏原螯虾AFLP分析的最佳反应条件,能够得到丰富稳定的带纹.而预扩增产物稀释倍数对选择性扩增没有明显影响.该体系的构建为AFLP技术在克氏原螯虾相关研究中的应用奠定了基础.
이극씨원오하DNA위재료,대AFLP분석중적기인조매절시간、선택성확증중예확증산물희석배수、Mg~(2+)농도등3개인소진행료비교.결과표명,기인조용EcoR I화MseI쌍매절5h,선택성확증적PCR반응체계중Mg~(2+)1.5 mmol/L시진행극씨원오하AFLP분석적최가반응조건,능구득도봉부은정적대문.이예확증산물희석배수대선택성확증몰유명현영향.해체계적구건위AFLP기술재극씨원오하상관연구중적응용전정료기출.
By using genomic DNA of Procambarus clarkii, the time for DNA double enzymes digestion reaction,the dilution multiple for the pre-amplification production and the concentration of Mg~(2+ )in the selective amplification reactions system were analyzed. The results showed using 5h for double enzymes digestion and Mg~(2+) 1.5 mmol/L were the best for AFLP analysis of P. clarkii. And no change was found when adjusting the dilution multiple for the pre-amplification production. The establishment of the system might lay a foundation for the application of AFLP techniques to the relative research of P. clarkii.