CAJ | 학술논문

目前蔬菜中氨基甲酸酯类农药残留普遍采用液相色谱柱后衍生法(HPLC)进行测定,研究液相色谱-质谱法(LC-MS/MS)测定蔬菜中7种常用氨基甲酸酯类农药,并与高效液相色谱柱后衍生法进行比较.液相色谱柱后衍生法是经典方法,重现性好,但是检测周期长,衍生试剂价格昂贵且稳定性差;LC-MS/MS法保留时间短,灵敏度高,操作简便,实验过程快,是氨基甲酸酯类农药检测发展的方向.
목전소채중안기갑산지류농약잔류보편채용액상색보주후연생법(HPLC)진행측정,연구액상색보-질보법(LC-MS/MS)측정소채중7충상용안기갑산지류농약,병여고효액상색보주후연생법진행비교.액상색보주후연생법시경전방법,중현성호,단시검측주기장,연생시제개격앙귀차은정성차;LC-MS/MS법보류시간단,령민도고,조작간편,실험과정쾌,시안기갑산지류농약검측발전적방향.
In the present study,7 carbamate pesticides in vegetables were determined using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS),and this method was compared with commonly used HPLC with post-column derivatization method.Results showed that the analytes were well separated using both methods,which had good figures of merit.HPLC with post-column derivatization method is considered as a classical method having the advantage of good repeatability and the disadvantages of time-consuming operation,expensive derivative reagent cost and poor stability;in contrast,LCMS/MS,thanks to its short retention time,high sensitivity,simple and easy operation,and rapidity,is more competitive for determination of carbamate pesticides in vegetables.