CAJ | 학술논문

探索了钝顶螺旋藻对D-氨基半乳糖(D-GalN)所致急性肝损伤的防护作用和体内抗氧化效果.ICR系小鼠在饲喂一周AIN-93~G标准饲料或含质量分数5 %钝顶螺旋藻粉末的实验饲料后,第七天腹部注射D-GalN(300 mg/kg)建立急性肝损伤模型.血清谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性、肝组织丙二醛(MDA)、还原型谷胱甘肽(GSH)以及维生素C水平在6 h后通过专用试剂盒或TBARS、DTNB和DNPH法分别测定.D-GalN处理显著提高血清GOT和GPT活性以及肝MDA水平,并显著降低了肝GSH和维生素C含量,而饲喂含螺旋藻饲料能够显著地抑制这些变化.研究结果表明:钝项螺旋藻具有显著的体内抗氧化效果,对D-GalN所致肝损伤具有很好的防护作用.
탐색료둔정라선조대D-안기반유당(D-GalN)소치급성간손상적방호작용화체내항양화효과.ICR계소서재사위일주AIN-93~G표준사료혹함질량분수5 %둔정라선조분말적실험사료후,제칠천복부주사D-GalN(300 mg/kg)건립급성간손상모형.혈청곡초전안매(GOT)화곡병전안매(GPT)활성、간조직병이철(MDA)、환원형곡광감태(GSH)이급유생소C수평재6 h후통과전용시제합혹TBARS、DTNB화DNPH법분별측정.D-GalN처리현저제고혈청GOT화GPT활성이급간MDA수평,병현저강저료간GSH화유생소C함량,이사위함라선조사료능구현저지억제저사변화.연구결과표명:둔항라선조구유현저적체내항양화효과,대D-GalN소치간손상구유흔호적방호작용.
In this study, the anti-oxidant and hepato-protective effect of Spirulina plantensis on D-galactosamine (D-GalN) induced liver injury in mice were investigated. ICR mice were fed a control diet (AIN-93G) or a diet contaning 5% Spirulina plantensis for one week and on the last day of feeding the mice were treated with D-GalN (300 mg/kg body weight, i. p.). The serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, hepatic malondialdehyde (MDA), reduced glutathione (GSH) and vitamin C levels were determined 6 h later by kits or by the method of TBARS, DTNB or DNPH, respectively. It was found that D-GalN induced increased in serum GOT, GPT and hepatic MDA level, but decreased in the GSH and vitamin C content. The results presented here demonstrated that Spirulina plantensis exhibited a significant antioxidant and protective on the D-GalN induced hepatotoxicity.