CAJ | 학술논문

利用PDA培养法从黑龙江省黑河、绥化和佳木斯等3个地区大豆发病田中,共分离和纯化出135个菌核病病原菌分离物,并对菌落的形状、生长速率、菌核重量和子囊盘等形态学特性进行了分析.结果表明:在菌落的形状和生长速率方面,不同地区的分离物群体间差异极显著,同一地区内各个分离物的表现一致性较好;在菌核重量和子囊盘形态特性方面,不同病原菌分离物产生的菌核的数量、大小、重量及子囊盘形态明显不同.不同采样地点的大豆菌核病病原菌菌落的形状和生长速率存在区域专化性,即3个采样地点的菌落形状和生长速率存在明显差异,而菌核重量和子囊盘形态特性不存在区域专化性.
이용PDA배양법종흑룡강성흑하、수화화가목사등3개지구대두발병전중,공분리화순화출135개균핵병병원균분리물,병대균락적형상、생장속솔、균핵중량화자낭반등형태학특성진행료분석.결과표명:재균락적형상화생장속솔방면,불동지구적분리물군체간차이겁현저,동일지구내각개분리물적표현일치성교호;재균핵중량화자낭반형태특성방면,불동병원균분리물산생적균핵적수량、대소、중량급자낭반형태명현불동.불동채양지점적대두균핵병병원균균락적형상화생장속솔존재구역전화성,즉3개채양지점적균락형상화생장속솔존재명현차이,이균핵중량화자낭반형태특성불존재구역전화성.
Soybean white mold is one of the popular diseases threatening soybean production,and causes serious yield decline.Morphological trait of soybean Sclerotinia sclerotiorum isolates wasn't clear for soybean studiers.For deepening the understanding of pathogen isolates,we separated a total of 135 isolates of Sclerotinia pathogens from three regions,including Heihe,Jiamusi and Suihua of Heilongjiang Province through PDA method.The objectives of the present study were to analyze the morphological trait of soybean Sclerotinia pathology.In this study,many morphology traits,such as colony shape,growth rate,sclerotia weight,ascus morphology,were measured.The results showed that the shape of the colony area and growth rate was significantly different among three sampling locations,and similar within each sampling locations.Other morphological trait of different isolates including the number,size,weight,of sclerotia,and morphological trait of apothecium,was significantly different whether among or within sampling locations.Results suggested that shape of the colony area and growth rate of isolates was specific to sampling location,while other morphological traits of different isolates including the number,size,weight,of sclerotia,and morphological trait of apothecium,wasn't specific to sampling location.