CAJ | 학술논문

细胞膜表面糖复合物的糖链结构与肿瘤细胞增殖、侵染、转移等发展过程密切相关.凝集素芯片技术的出现实现了对癌症的糖组进行快速、高通量的检测.通过模式细胞系PANC-1证明了构建的凝集素芯片体系的准确性、重复性、特异性,应用这一芯片体系初步检测了几种癌细胞系(HT-29、SGC-7901、BEL-7402、H460)的膜表面糖链表达.这几种癌细胞系表面都有唾液酸、乙酰葡萄糖,葡萄糖、乙酰半乳糖/半乳糖、甘露糖等糖链.根据实验结果.推测它们的细胞膜表面α1-6岩藻糖链表达水平可能较高,而α1-3岩藻糖链表达水平较低:这些聚糖可能是癌症潜在的标志物.凝集素芯片有助于推动癌细胞膜表面糖链的快速分析和筛选出癌症相关的糖链标志物.
세포막표면당복합물적당련결구여종류세포증식、침염、전이등발전과정밀절상관.응집소심편기술적출현실현료대암증적당조진행쾌속、고통량적검측.통과모식세포계PANC-1증명료구건적응집소심편체계적준학성、중복성、특이성,응용저일심편체계초보검측료궤충암세포계(HT-29、SGC-7901、BEL-7402、H460)적막표면당련표체.저궤충암세포계표면도유타액산、을선포도당,포도당、을선반유당/반유당、감로당등당련.근거실험결과.추측타문적세포막표면α1-6암조당련표체수평가능교고,이α1-3암조당련표체수평교저:저사취당가능시암증잠재적표지물.응집소심편유조우추동암세포막표면당련적쾌속분석화사선출암증상관적당련표지물.
Glycan changes on the cell surface have been involved in a series of tumour progression process, such as proliferation, invasion and haematogenous metastasis. The lectin microarray makes it possible to analyze glycan profiling on the cancer cell in a rapid and high-throughput approach. By model cell lines PANC-1, we demonstrated the reproducibility, accuracy and specificity of our lectin microarray. Then the array was used to examinate dynamic glycosylation differences on the surfaces of cell lines. The results showed the microarray has a good characteristic. The array results indicate that the characterization of membrane sugars is sialic acid, lewis~x, GlcNAc / glucose, GalNAc / galactose, mannose. They probably express mostly α1-6Fucose and have few α1-3fucose. These glycans may be potential as cancer cell markers. This work could greatly contribute to the rapid analysis of cancer cell-surface glycan profiling and the discovery of cancer-related glycan markers.