CAJ | 학술논문

目的　探讨增龄对大鼠骨骼肌细胞葡萄糖转运蛋白4(glucose transporter 4, GLUT4)的影响。　方法　SD实验大鼠分为2组：青年组（3月龄）和老年组（24月龄）各式各样0只。制务GLUT4羧基端正2肽的多克隆抗体，利用Western印迹法检测2组大鼠骨骼肌细胞GLUT4蛋白含量，并检测大鼠尾静脉血糖。　结果　老年组大鼠的血糖（5.6±0.5 mmol/L)略高于青年组大鼠（4.5±0.5 mmol/L)，但差异无显著性；青年组大鼠骨骼肌细胞内GLUT4相对含量为109.62±12.25，而老年组为86.46±8.25，差异有显著性（P<0.05)。　结论　增龄可引起大鼠骨骼肌细胞GLUT4蛋白含量明显减少，使骨骼肌细胞对葡萄糖的转运发生障碍，这可能是老年人易产生胰岛素抵抗的机制之一。
목적　탐토증령대대서골격기세포포도당전운단백4(glucose transporter 4, GLUT4)적영향。　방법　SD실험대서분위2조：청년조（3월령）화노년조（24월령）각식각양0지。제무GLUT4최기단정2태적다극륭항체，이용Western인적법검측2조대서골격기세포GLUT4단백함량，병검측대서미정맥혈당。　결과　노년조대서적혈당（5.6±0.5 mmol/L)략고우청년조대서（4.5±0.5 mmol/L)，단차이무현저성；청년조대서골격기세포내GLUT4상대함량위109.62±12.25，이노년조위86.46±8.25，차이유현저성（P<0.05)。　결론　증령가인기대서골격기세포GLUT4단백함량명현감소，사골격기세포대포도당적전운발생장애，저가능시노년인역산생이도소저항적궤제지일。
Objective　To investigate the difference of glucose transporter 4 (GLUT4) protein content in skeletal muscles of young and aged rats. 　Methods　Twenty SD rats were divided into two groups, namely young rats (3 months) and aged rats (24 months). The content of GLUT4 protein in skeletal muscle cells was detected by Western blot analysis in two group rats. The polyclonal antibody against GLUT4 protein was obtained from GLUT4 protein immunized rabbits. Blood glucose level of two groups was also detected. Results The relative content of GLUT4 protein in skeletal muscle cells of aged rats (86.46?.25) decreased 21.1% as compared with young one (109.62?2.25) (P?0.05). There was no significant difference in blood glucose level between two groups. Conclusions The decreased content of GLUT4 protein in skeletal muscles cells may associated with age-related insulin resistance in aged.