CAJ | 학술논문

目的探讨125I-脱氧尿嘧啶核苷(125I-UdR)在淋巴瘤细胞Raji和Dandi中的特异性摄取及其杀伤效应.方法测量Raji、Daudi细胞和细胞核在含不同放射性浓度125I-UdR的培养液中培养不同时间后的活度;用噻唑蓝(MTT)实验和碘化丙啶(PI)染色周期分析评价125I-UdR对Raji和Daudi细胞的杀伤作用.结果 Raji和Daudi细胞摄取125I-UdR的量明显高于Na125I对照组(P<0.05),在100 kBq/ml浓度时,Raji和Daudi细胞摄取125I-UdR的量分别为(14414±95)和(6916±53.69)Bq/106细胞,而对Na125I的摄取量分别为(68±3.8)和(324±32.8)Bq/106细胞;细胞和细胞核中125I-UdR的量随培养基中125I-UdR放射性浓度以及培养时间的增加而增加;125I-UdR组的存活分数明显低于Na125I对照组(P<0.05),以500 kBq/ml浓度培养48 h时,Raji和Dandi细胞125I-UdR组的存活分数分别为(19.78 ±1.39)%和(43.17 ±2.69)%,而Na125I组的存活分数分别为(79.10 ±1.79)%和(80.36±6.12)%;细胞存活分数有随培养基中放射性浓度增加而降低的趋势.结论 125I-UdR可被Raji和Daudi细胞特异性摄取并进入细胞核中,进而杀死细胞,其作用具有明显的时间-效应和剂量-效应关系.
목적 탐토125I-탈양뇨밀정핵감(125I-UdR)재림파류세포Raji화Dandi중적특이성섭취급기살상효응.방법 측량Raji、Daudi세포화세포핵재함불동방사성농도125I-UdR적배양액중배양불동시간후적활도;용새서람(MTT)실험화전화병정(PI)염색주기분석평개125I-UdR대Raji화Daudi세포적살상작용.결과 Raji화Daudi세포섭취125I-UdR적량명현고우Na125I대조조(P<0.05),재100 kBq/ml농도시,Raji화Daudi세포섭취125I-UdR적량분별위(14414±95)화(6916±53.69)Bq/106세포,이대Na125I적섭취량분별위(68±3.8)화(324±32.8)Bq/106세포;세포화세포핵중125I-UdR적량수배양기중125I-UdR방사성농도이급배양시간적증가이증가;125I-UdR조적존활분수명현저우Na125I대조조(P<0.05),이500 kBq/ml농도배양48 h시,Raji화Dandi세포125I-UdR조적존활분수분별위(19.78 ±1.39)%화(43.17 ±2.69)%,이Na125I조적존활분수분별위(79.10 ±1.79)%화(80.36±6.12)%;세포존활분수유수배양기중방사성농도증가이강저적추세.결론 125I-UdR가피Raji화Daudi세포특이성섭취병진입세포핵중,진이살사세포,기작용구유명현적시간-효응화제량-효응관계.
Objective To evaluate the killing effect and the uptake of 125I-UdR on human lymphoma Raji and Daudi cell lines. Methods The amount of 125I-UdR in the cells and cell nuclei were determined after incubation of different time in RPMI 1640 culturing medium containing different concentrations of 125I-UdR. The killing effects of 125I-UdR on Raji and Daudi cell lines were estimated through MTT assay and cell cycle was analyzed by propidium iodide (PI) staining. Results The amounts of 125I-UdR in Raji and Dandi cells and cell nuclei were much higher than that of Na125I(P < 0.05). The amounts of 125l-UdR in Raji and Daudi cells were 14414±95 and (6916± 53.69) Bq/106 cell when the concentration was 100 kBq/ml. The amounts of Na125I were 68± 3.8 and (324±32.8) Bq/106 cell. The uptake of 125I-UdR in Raji and Daudi cells and cell nuclei increased with the 125I-UdR concentration and incubated time. The cell surviving fractions of 125I-UdR groups was much lower than that of Na125I groups (P < 0.05). When the concentration was 500 kBq/ml and incubated time was 48 hours, the Raji and Dandi cell surviving fractions of125I-UdR groups were (19.78 ± 1.39)% and (43.17 ± 2.69) % ;those of Na125I groups were (79.10 ± 1.79) % and (80.36 ± 6.12) %. The surviving fractions of 125I-UdR groups reduced with the 125I-UdR concentration. Conclusions 125I-UdR can be specially ingested by Raji and Daudi cells and incorporated into DNA, then the cells will be killed. The uptake of 125I-UdR is dose and time dependent.