中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
7期
475-479
,共5页
蒋金玲%刘卫仁%于颖彦%倪培华%吴建林%计骏%张佳年%陈雪华%刘炳亚%朱正纲
蔣金玲%劉衛仁%于穎彥%倪培華%吳建林%計駿%張佳年%陳雪華%劉炳亞%硃正綱
장금령%류위인%우영언%예배화%오건림%계준%장가년%진설화%류병아%주정강
新生血管化,病理性%新生血管化,生理性%疾病模型,动物%图像处理,计算机辅助
新生血管化,病理性%新生血管化,生理性%疾病模型,動物%圖像處理,計算機輔助
신생혈관화,병이성%신생혈관화,생이성%질병모형,동물%도상처리,계산궤보조
Neovascularization,pathologic%Neovascularization,physiologic%Disease models,animal%Image processing,computer-assisted
目的 利用显微图像分析法研究肿瘤新生血管相关模型.方法 以人脐静脉内皮细胞和胃癌细胞系建立体外小管形成模型与血管拟态形成模型,以鸡的受精卵建立鸡胚尿囊膜活体血管形成模型,观察抑癌基因IRX1对血管形成和血管拟态形成的影响.显微镜所摄数字图片通过Image Pro Plus(IPP)专业图像软件进行分析.通过计数点和线长度分析体外小管形成;通过吸光度法测量血管拟态的PAS阳性物质;通过计数法和角度测量法计算鸡胚尿囊膜血管数目.结果 IRX1转基因组与空载体组及空白对照组相比,均显示了抑制血管形成能力,其小管形成数量三组分别为(12.80±3.83)、(29.00±5.34)和(28.20±4.32)个(P<0.01),三组的小管连接点分别为(13.20±2.59)、(25.00±2.24)和(24.60±3.21)个(P<0.01),三组的小管长度分别为(821.5±12.5)、(930.9±13.5)和(948.4±18.1)μm(P=0.022).IRX1基因转染组的PAS阳性物质吸光度值(3606±363)显著低于空载体组(14 200±1251)与空白对照组(15 043±1220,P<0.01).IRX1基因转染组的鸡胚尿囊膜血管形成数量明显少于空载体组与空白对照组,三组角测量血管数量分别为(6.41±2.60)、(10.27±2.65)和(9.18±1.99)个(P<0.01).结论 体外血管形成、血管拟态模型和鸡胚活体血管形成模型研究均显示,IRX1基因转染可以明显抑制新生血管形成;上述模型适用于以血管为靶点的基因干预或者药物筛选研究;专业图像分析软件在肿瘤血管形成的定量分析研究中可发挥一定的作用.
目的 利用顯微圖像分析法研究腫瘤新生血管相關模型.方法 以人臍靜脈內皮細胞和胃癌細胞繫建立體外小管形成模型與血管擬態形成模型,以鷄的受精卵建立鷄胚尿囊膜活體血管形成模型,觀察抑癌基因IRX1對血管形成和血管擬態形成的影響.顯微鏡所攝數字圖片通過Image Pro Plus(IPP)專業圖像軟件進行分析.通過計數點和線長度分析體外小管形成;通過吸光度法測量血管擬態的PAS暘性物質;通過計數法和角度測量法計算鷄胚尿囊膜血管數目.結果 IRX1轉基因組與空載體組及空白對照組相比,均顯示瞭抑製血管形成能力,其小管形成數量三組分彆為(12.80±3.83)、(29.00±5.34)和(28.20±4.32)箇(P<0.01),三組的小管連接點分彆為(13.20±2.59)、(25.00±2.24)和(24.60±3.21)箇(P<0.01),三組的小管長度分彆為(821.5±12.5)、(930.9±13.5)和(948.4±18.1)μm(P=0.022).IRX1基因轉染組的PAS暘性物質吸光度值(3606±363)顯著低于空載體組(14 200±1251)與空白對照組(15 043±1220,P<0.01).IRX1基因轉染組的鷄胚尿囊膜血管形成數量明顯少于空載體組與空白對照組,三組角測量血管數量分彆為(6.41±2.60)、(10.27±2.65)和(9.18±1.99)箇(P<0.01).結論 體外血管形成、血管擬態模型和鷄胚活體血管形成模型研究均顯示,IRX1基因轉染可以明顯抑製新生血管形成;上述模型適用于以血管為靶點的基因榦預或者藥物篩選研究;專業圖像分析軟件在腫瘤血管形成的定量分析研究中可髮揮一定的作用.
목적 이용현미도상분석법연구종류신생혈관상관모형.방법 이인제정맥내피세포화위암세포계건입체외소관형성모형여혈관의태형성모형,이계적수정란건립계배뇨낭막활체혈관형성모형,관찰억암기인IRX1대혈관형성화혈관의태형성적영향.현미경소섭수자도편통과Image Pro Plus(IPP)전업도상연건진행분석.통과계수점화선장도분석체외소관형성;통과흡광도법측량혈관의태적PAS양성물질;통과계수법화각도측량법계산계배뇨낭막혈관수목.결과 IRX1전기인조여공재체조급공백대조조상비,균현시료억제혈관형성능력,기소관형성수량삼조분별위(12.80±3.83)、(29.00±5.34)화(28.20±4.32)개(P<0.01),삼조적소관련접점분별위(13.20±2.59)、(25.00±2.24)화(24.60±3.21)개(P<0.01),삼조적소관장도분별위(821.5±12.5)、(930.9±13.5)화(948.4±18.1)μm(P=0.022).IRX1기인전염조적PAS양성물질흡광도치(3606±363)현저저우공재체조(14 200±1251)여공백대조조(15 043±1220,P<0.01).IRX1기인전염조적계배뇨낭막혈관형성수량명현소우공재체조여공백대조조,삼조각측량혈관수량분별위(6.41±2.60)、(10.27±2.65)화(9.18±1.99)개(P<0.01).결론 체외혈관형성、혈관의태모형화계배활체혈관형성모형연구균현시,IRX1기인전염가이명현억제신생혈관형성;상술모형괄용우이혈관위파점적기인간예혹자약물사선연구;전업도상분석연건재종류혈관형성적정량분석연구중가발휘일정적작용.
Objective To establish experimental models for tumor neovascularization and to apply quantitative digital imaging analysis in the study. Methods An endothelial tube formation model was established by human umbilical vein endothelial cells (HUVECs). A vasculogenic mimicry model was established by SGC-7901 gastric cancer cell line. Fertilized eggs were used to establish a chorioallantoic membrane angiogenesis model. Using gene transfection experiment, IRX1 tumor suppressor gene was chosen as a therapeutic target. Image Pro Plus (IPP) analysis software was used for digital vascular images analysis with parameters including points, lines, angles and integral absorbance (IA) for the tubular formation or vasculogenic mimicry.Results Digital image analysis by IPP showed that HUVEC tubular formation was significantly inhibited in IRX1 transfectant, compared with controls. The tubular numbers in three groups were 12.80±3.83, 29.00±5.34 and 28.20±4.32(P<0.01).The connection points of tubules in three groups were 13.20±2.59, 25.00±2.24 and 24.60±3.21(P<0.01). The tubular lengths of three groups were (821.5±12.5), (930.9±13.5)and(948.4±18.1)μm(P=0.022). The IA values of PAS stain in three groups were 3606±363, 14 200±1251 and 15 043±1220 (P<0.01). In chick chorioallantoic membrane model, the angular numbers of tubules in three groups were 6.41±2.60, 10.27±2.65 and 9.18±1.99(P<0.01). Conclusions The endothelial tube formation model, vasculogenic mimicry model and chorioallantoic membrane angiogenesis model are useful for gene therapy and drug screening with targeting neoplastic vascularization. Professional image analysis software may greatly facilitate the quantitative analysis of tumor neovascularization.
