中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2009年
10期
663-667
,共5页
尹洪芳%李挺%张虹%张爽
尹洪芳%李挺%張虹%張爽
윤홍방%리정%장홍%장상
乳腺肿瘤%癌%导管%癌%小叶状%钙黏着糖蛋白类%连环素类%免疫表型分型%诊断%鉴别
乳腺腫瘤%癌%導管%癌%小葉狀%鈣黏著糖蛋白類%連環素類%免疫錶型分型%診斷%鑒彆
유선종류%암%도관%암%소협상%개점착당단백류%련배소류%면역표형분형%진단%감별
Breast neoplasms%Carcinoma%ductal%breast%Carcinoma%lobular%Cadherins%Catenins%Immunophenotyping%Diagnosis%differential
目的 探讨组织形态上易混淆的浸润性导管癌(IDC)与浸润性小叶癌(ILC)的鉴别诊断方法 .方法 收集北京大学第一医院1998年1月至2001年12月4年间普通外科诊治并有完整随访资料的IDC Ⅰ级24例、ILC 12例和具有混合性导管-小叶特征的浸润性癌(简称混合癌)14例共50例原发性乳腺癌患者资料和标本.采用EnVision法免疫组织化学染色检测E-钙黏蛋白(E-cad)、p120连环蛋白(p120ctn)、上皮膜蛋白(EMP)1和DVL1.结果 E-cad在IDC Ⅰ级和ILC中的阳性率分别为83.3%(20/24)和0,其差异有统计学意义(P<0.01);p120ctn在IDC Ⅰ级中阳性率为100.0%,且均为胞膜着色,在ILC中亦为12例均阳性,但均为细胞质着色;EMP1和DVL1在IDC Ⅰ级和ILC中的阳性分别为95.8%(23/24)和12例,54.2%(13/24)和5例,其差异无统计学意义(P>0.05).E-cad和p120ctn联合使用将14例混合痛确诊为IDC 12例和ILC 2例.结论 E-cad和p120ctn联合使用可以鉴别易混淆的IDC和ILC,使组织分型更准确.EMP1、DVL1不能作为鉴别IDC和ILC的指标.
目的 探討組織形態上易混淆的浸潤性導管癌(IDC)與浸潤性小葉癌(ILC)的鑒彆診斷方法 .方法 收集北京大學第一醫院1998年1月至2001年12月4年間普通外科診治併有完整隨訪資料的IDC Ⅰ級24例、ILC 12例和具有混閤性導管-小葉特徵的浸潤性癌(簡稱混閤癌)14例共50例原髮性乳腺癌患者資料和標本.採用EnVision法免疫組織化學染色檢測E-鈣黏蛋白(E-cad)、p120連環蛋白(p120ctn)、上皮膜蛋白(EMP)1和DVL1.結果 E-cad在IDC Ⅰ級和ILC中的暘性率分彆為83.3%(20/24)和0,其差異有統計學意義(P<0.01);p120ctn在IDC Ⅰ級中暘性率為100.0%,且均為胞膜著色,在ILC中亦為12例均暘性,但均為細胞質著色;EMP1和DVL1在IDC Ⅰ級和ILC中的暘性分彆為95.8%(23/24)和12例,54.2%(13/24)和5例,其差異無統計學意義(P>0.05).E-cad和p120ctn聯閤使用將14例混閤痛確診為IDC 12例和ILC 2例.結論 E-cad和p120ctn聯閤使用可以鑒彆易混淆的IDC和ILC,使組織分型更準確.EMP1、DVL1不能作為鑒彆IDC和ILC的指標.
목적 탐토조직형태상역혼효적침윤성도관암(IDC)여침윤성소협암(ILC)적감별진단방법 .방법 수집북경대학제일의원1998년1월지2001년12월4년간보통외과진치병유완정수방자료적IDC Ⅰ급24례、ILC 12례화구유혼합성도관-소협특정적침윤성암(간칭혼합암)14례공50례원발성유선암환자자료화표본.채용EnVision법면역조직화학염색검측E-개점단백(E-cad)、p120련배단백(p120ctn)、상피막단백(EMP)1화DVL1.결과 E-cad재IDC Ⅰ급화ILC중적양성솔분별위83.3%(20/24)화0,기차이유통계학의의(P<0.01);p120ctn재IDC Ⅰ급중양성솔위100.0%,차균위포막착색,재ILC중역위12례균양성,단균위세포질착색;EMP1화DVL1재IDC Ⅰ급화ILC중적양성분별위95.8%(23/24)화12례,54.2%(13/24)화5례,기차이무통계학의의(P>0.05).E-cad화p120ctn연합사용장14례혼합통학진위IDC 12례화ILC 2례.결론 E-cad화p120ctn연합사용가이감별역혼효적IDC화ILC,사조직분형경준학.EMP1、DVL1불능작위감별IDC화ILC적지표.
Objective To study the diagnostic usefulness of immunohistochemical markers in distinguishing between invasive ductal carcinoma and invasive lobular carcinoma of breast. Methods Twenty-four cases of grade Ⅰ invasive ductal carcinoma, 12 cases of classic invasive lobular carcinoma and 14 cases of invasive carcinoma with mixed ductal and lobular features were retrieved from the archival files of Peking University First Hospital during the period from January, 1998 to December, 2001. Immunohistochemical study for E-cadherin, p120 catenin,epithelia membrane protein 1 ( EMP1 ) and DVL1 was performed. Results The positivity rates for E-cadherin in grade Ⅰ invasive ductal carcinoma and classic invasive lobular carcinoma were 83.3% (20/24) and 0, respectively (P<0.01). The positivity rates for p120 catenin were 100% in both grade 1 invasive ductal carcinoma (membranous staining) and classic invasive lobular carcinoma (cytoplasmic staining). The positivity rates for EMP1 and DVL1 in grade Ⅰ invasive ductal carcinoma were 95. 8% (23/24) and 54. 2% ( 13/24), respectively; while those in classic invasive lobular carcinoma were 12 and 5 cases, respectively. Conclusions E-cadherin and p120 catenin are useful immunomarkers for distinguishing between invasive ductal carcinoma and invasive lobular carcinoma. On the other hand, EMP1 and DVL1 are of limited value in this respect.
