中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2010年
3期
241-243
,共3页
白内障%晶状体蛋白%western%blot%混浊度%相关性
白內障%晶狀體蛋白%western%blot%混濁度%相關性
백내장%정상체단백%western%blot%혼탁도%상관성
Cataract%Crystalline%Westernblot%Opacities%Correlation
目的 分析晶状体不溶性αB蛋白含量与年龄及混浊度的相关性,探讨该蛋白在白内障发病机制的作用.方法 收集80只眼晶状体标本,按年龄段分为老年组(A组,30只眼),中壮年组(B组,30只眼)及青年组(C组,20只眼);用LOCS(Ⅲ)分级法,对晶状体核混浊度进行分级,用westem blot法检测晶状体αB蛋白含量,用Spearman法对年龄、混浊度及αB蛋白含量进行相关性分析.结果 三组患者的αB蛋白印迹条带灰度值分别为(149.6±18.2)、(126.5±15.4)及(98.1±10.6),组间两两差异具有统计学意义(均P<0.05);年龄与混浊度的相关系数r=0.918,年龄与αB蛋白表达含量相关系数r=0.652,混浊度与αB蛋白表达含量相关系数r=0.620,均P=0.00.结论 不溶性αB晶状体蛋白与年龄及晶状体混浊两两呈正相关性,前者可能在老年性白内障的发病机制当中起到一定的作用.
目的 分析晶狀體不溶性αB蛋白含量與年齡及混濁度的相關性,探討該蛋白在白內障髮病機製的作用.方法 收集80隻眼晶狀體標本,按年齡段分為老年組(A組,30隻眼),中壯年組(B組,30隻眼)及青年組(C組,20隻眼);用LOCS(Ⅲ)分級法,對晶狀體覈混濁度進行分級,用westem blot法檢測晶狀體αB蛋白含量,用Spearman法對年齡、混濁度及αB蛋白含量進行相關性分析.結果 三組患者的αB蛋白印跡條帶灰度值分彆為(149.6±18.2)、(126.5±15.4)及(98.1±10.6),組間兩兩差異具有統計學意義(均P<0.05);年齡與混濁度的相關繫數r=0.918,年齡與αB蛋白錶達含量相關繫數r=0.652,混濁度與αB蛋白錶達含量相關繫數r=0.620,均P=0.00.結論 不溶性αB晶狀體蛋白與年齡及晶狀體混濁兩兩呈正相關性,前者可能在老年性白內障的髮病機製噹中起到一定的作用.
목적 분석정상체불용성αB단백함량여년령급혼탁도적상관성,탐토해단백재백내장발병궤제적작용.방법 수집80지안정상체표본,안년령단분위노년조(A조,30지안),중장년조(B조,30지안)급청년조(C조,20지안);용LOCS(Ⅲ)분급법,대정상체핵혼탁도진행분급,용westem blot법검측정상체αB단백함량,용Spearman법대년령、혼탁도급αB단백함량진행상관성분석.결과 삼조환자적αB단백인적조대회도치분별위(149.6±18.2)、(126.5±15.4)급(98.1±10.6),조간량량차이구유통계학의의(균P<0.05);년령여혼탁도적상관계수r=0.918,년령여αB단백표체함량상관계수r=0.652,혼탁도여αB단백표체함량상관계수r=0.620,균P=0.00.결론 불용성αB정상체단백여년령급정상체혼탁량량정정상관성,전자가능재노년성백내장적발병궤제당중기도일정적작용.
Objective To observe whether the age is related to insoluble alphaB-erystallin and lens opacities in senile cataract patients so as to explore the mechanism of the disease.Methods Thirty lens from patients aged 65-82 years old,30 lens from that of 45-64 and 20 lens from wrongful death youth were collect- ed.The grade of lens opacities was determined by LOCS (Ⅲ),the quantities of alphaB-erystallin were tested through western blot.The relationship between ages,alphaB-crystallin and lens opacities were analyzed by Spearman correlation method.Results The gray value of alphaB-rystallin in three groups were 149.6±18.2. 126.5±15.4 and 98.1±10.6 respectively,the difference bctwee groups was statistically significant (all P=0. 000).The correction coefficient between age and lens opacities was 0.918,that of age and lens opacities was0.652,and that of age and alphaB-crystallin was 0.620 (all P=0.000).Conclusions Age,insoluble al- phaB-crystallin and lens opacities present positive correlation in senile cataract patients,indicating that the crystalline insoluble alphaB-erystallin may play a key role in senile cataract.
