CAJ | 학술논문

目的研究人血高密度脂蛋白(HDL)注射液对喂高胆固醇饲料家兔主动脉粥样指纹病变的影响.方法实验家兔给与1%胆固醇饲料8周.在此期间,HDL组每只家兔每周静脉注射50 mg人血HDL;洛伐他汀组每只家兔每天皮下注射10mg洛伐他汀;安慰剂组每只家兔每天静脉注射20mL生理盐水.人血HDL注射液由清华紫光古汉生物制药集团有限公司生产并提供.结果在试验期间,各组实验动物喂高胆固醇饲料后血脂水平均有升高.第八周后,实验动物主动脉内膜表面富脂粥样病变面积比分别为:安慰剂组32.6%±21.7%(x±s,下同);HDL组9.16%±7.87%;洛伐他汀组20.8%±13.1%.血清总胆固醇水平分别为:安慰剂组10.0±2.30g/L;HDL组2.92±1.41g/L;洛伐他汀组3.74±1.73g/L.血清HDL胆固醇水平分别为:安慰剂组0.43±0.12g/L:HDL组 0.62±0.23g/L;洛伐他汀组0.23±0.14g/L.HDL组血管壁胆固醇含量比安慰剂组和洛伐他汀组都明显降低,说明人血HDL具有比洛伐他汀更有效的抗动脉粥样硬化病变作用.结论人血HDL能够有效地抑制喂胆固醇饲料家兔主动脉粥样病变的作用,并能够调节血脂,减少动脉壁脂质沉积.
목적연구인혈고밀도지단백(HDL)주사액대위고담고순사료가토주동맥죽양지문병변적영향.방법실험가토급여1%담고순사료8주.재차기간,HDL조매지가토매주정맥주사50 mg인혈HDL;락벌타정조매지가토매천피하주사10mg락벌타정;안위제조매지가토매천정맥주사20mL생리염수.인혈HDL주사액유청화자광고한생물제약집단유한공사생산병제공.결과재시험기간,각조실험동물위고담고순사료후혈지수평균유승고.제팔주후,실험동물주동맥내막표면부지죽양병변면적비분별위:안위제조32.6%±21.7%(x±s,하동);HDL조9.16%±7.87%;락벌타정조20.8%±13.1%.혈청총담고순수평분별위:안위제조10.0±2.30g/L;HDL조2.92±1.41g/L;락벌타정조3.74±1.73g/L.혈청HDL담고순수평분별위:안위제조0.43±0.12g/L:HDL조 0.62±0.23g/L;락벌타정조0.23±0.14g/L.HDL조혈관벽담고순함량비안위제조화락벌타정조도명현강저,설명인혈HDL구유비락벌타정경유효적항동맥죽양경화병변작용.결론인혈HDL능구유효지억제위담고순사료가토주동맥죽양병변적작용,병능구조절혈지,감소동맥벽지질침적.
Aim The effects of in vivo administration of human high density lipoprotein (HDL) injection on the development of aortic streaks were studied in cholesterol-fed rabbits.Methods The rabbits received a 1% cholesterol-rich diet for 8 weeks. During this period, the HDL group was intravenously administered with 50 mg/week of HDL injection; the lovastatin group was administered with 10 mg/day of lovastatin via subcutaneous injection; the placebo group received normal saline (0.9% NaCl). The HDL injection was manufactured and provided by Tsinghua Unisplendour Guhan Bio-pharmaceutical Corporation Ltd. During the study, plasma lipid levels followed a similar profile in all groups to cholesterol-rich diet.Results At the completion of study, atherosclerotic-like lipid-rich lesions covered 32.6% 21.7 % (x ± s ), of the intima aortic surface in the placebo group, 9.1% ± 7.8 % in the HDL group, and 20.8 % ± 13.1% in the lovastatin group. The levels of plasma total cholesterol were 10.05 + 2.30, 2.93 ± 1.41, and 3.74 ± 1.73 g/L in placebo, HDL, and lovastatin groups,respectively. The levels of plasma high density lipoprotein cholesterol (HDLC)were0.43±0.12, 0.62±0.23, 0.23±0.14 g/L in placebo, HDL, and lovastatin groups, respectively. The value of total cholesterol deposited within vessel wall were significantly lower in the aortas of the HDL group than those in the placebo group and lovastatin group. Human HDL injection showed a more efficient inhibition of atherosclerotic lesions than lovastatin did.Conclusion The administration of human HDL injection to cholesterol-fed rabbits dramatically inhibits the extent of aortic fatty streaks, modifies plasma lipid, and lipid deposition in the arterial wall.