CAJ | 학술논문

用壳聚糖对多壁碳纳米管进行修饰,构建了一种用于固定血红蛋白的新型复合材料,并研究了血红蛋白在该碳纳米管上的电化学性质及其对过氧化氢的电催化活性.扫描电镜结果表明,壳聚糖修饰的多壁碳纳米管呈单一的纳米管状,并能均匀分散在玻碳电极表面.紫外光谱分析表明血红蛋白在该复合膜内能很好地保持其原有的二级结构.将该材料固定在玻碳电极上后,血红蛋白能成功地实现其直接电化学.根据峰电位差随着扫描的变化,计算得到血红蛋白在壳聚糖修饰的碳纳米管膜上的电荷转移系数为0.57,表观电子转移速率常数为7.02 s-1.同时,该电极对过氧化氢显示出良好的催化性能,电流响应信号与H2O2浓度在1.0×10-6 ～1.5×10-3 mol/L间呈线性关系,检出限为5.0×10-7 mol/L.修饰电极显示了良好的稳定性.
용각취당대다벽탄납미관진행수식,구건료일충용우고정혈홍단백적신형복합재료,병연구료혈홍단백재해탄납미관상적전화학성질급기대과양화경적전최화활성.소묘전경결과표명,각취당수식적다벽탄납미관정단일적납미관상,병능균균분산재파탄전겁표면.자외광보분석표명혈홍단백재해복합막내능흔호지보지기원유적이급결구.장해재료고정재파탄전겁상후,혈홍단백능성공지실현기직접전화학.근거봉전위차수착소묘적변화,계산득도혈홍단백재각취당수식적탄납미관막상적전하전이계수위0.57,표관전자전이속솔상수위7.02 s-1.동시,해전겁대과양화경현시출량호적최화성능,전류향응신호여H2O2농도재1.0×10-6 ～1.5×10-3 mol/L간정선성관계,검출한위5.0×10-7 mol/L.수식전겁현시료량호적은정성.
A novel composite material of multi-walled carbon nanotubes(MWCNTs) modified with chitosan was prepared to immobilize redox protein hemoglobin(Hb).The electrochemical characteristics of Hb immobilized on the modified MWCNTs and its electrocatalytic activity toward H2O2 were also studied.Scanning electron microscopy images showed that the modified MWCNTs exhibited a single nano tubiform and could well dispersed on the glassy carbon electrode.UV-Vis spectra indicated that Hb could keep its original secondary structure well in the composite film.Cyclic voltammogram of Hb-chitosan-MWCNTs film modified glassy carbon electrode showed a pair of well-defined and quasi-reversible redox peaks for Hb Fe(Ⅲ)/Fe(Ⅱ),indicating that a direct electron transfer between Hb and GC electrode occurred.The charge-transfer coefficient and the apparent electron-transfer rate constant for Hb on chitosan modified MWCNTs were calculated to be 0.57 and 7.02 s-1,respectively.The formal potential (V0′)of-0.324 V(vs SCE) was observed,which was a characteristic potential of Hb heme Fe(Ⅲ)/Fe(Ⅱ) redox couple.The dependence of formal potential (V0′) on solution pH indicated that one-proton transfer was coupled to each electron transfer in the direct electron-transfer reaction.Furthermore,the modified electrode showed excellent electrocatalytic activity toward H2O2.The electrocatalytic current signals were linear with the concentration of H2O2 in a wide range of 1.0×10-6-1.5×10-3 mol/L with a detection limit of 5.0×10-7 mol/L.The modified electrode also exhibited good stability in one week.