CAJ | 학술논문

为创制大穗型小麦种质材料,利用具有大穗多小穗性状的小麦-黑麦双二倍体材料"兰小黑"和普通小麦杂交得到一批大穗型衍生后代.综合采用基因组原位杂交(GISH)、SCAR标记、微卫星(SSR)和醇溶蛋白(A-PAGE)技术对这些大穗型后代中的8个单株进行分子细胞学鉴定.结果表明,GISH检测后代含2个外源信号;1RS特异SCAR标记检测后代均含有黑麦1.5 kb的1RS特征条带;醇溶蛋白检测后代都出现了黑麦碱基因Sec-1特征条带.筛选小麦21条染色体长短臂上各6对引物,结果发现只有1BS上的3对引物未扩增出1BS的条带,其余引物均扩增出了各自的相应条带.由此确定这8株小麦-黑麦大穗型衍生后代为1BL/1RS易位材料.
위창제대수형소맥충질재료,이용구유대수다소수성상적소맥-흑맥쌍이배체재료"란소흑"화보통소맥잡교득도일비대수형연생후대.종합채용기인조원위잡교(GISH)、SCAR표기、미위성(SSR)화순용단백(A-PAGE)기술대저사대수형후대중적8개단주진행분자세포학감정.결과표명,GISH검측후대함2개외원신호;1RS특이SCAR표기검측후대균함유흑맥1.5 kb적1RS특정조대;순용단백검측후대도출현료흑맥감기인Sec-1특정조대.사선소맥21조염색체장단비상각6대인물,결과발현지유1BS상적3대인물미확증출1BS적조대,기여인물균확증출료각자적상응조대.유차학정저8주소맥-흑맥대수형연생후대위1BL/1RS역위재료.
In order to creat a new germplasm with big ear, a large number of derivatives with big ear were obtained from a cross between wheat and Lanxiaohei, a wheat-rye double diploid with big ear and multi-small spikelets. GISH、SCAR、SSR and Gliadin analysis were conducted to detect the rye fragments in the offspring lines of the wheat-rye with big ear. Genome in Situ Hybridization (GISH) indicated that the offspring contained 2 exogenous signals. SCAR displaying a single 1.5 kb rye band could be amplified in the offspring and its father Lanxiaohei. Gliadin observed secaline specific band in the offspring and its father Lanxiaohei. Screening with 6 pairs of SSR primers on the long arm and short arm of 21 chromosomes, respectively, displayed that only three primers in 1BS could not be amplified, this consequentially confirmed that the offspring lines of the wheat-rye with big ear was 1BL/1RS translocation.