CAJ | 학술논문

濒危植物沙冬青Ammopiptanthus mongolicus为亚洲中部荒漠地区唯一的常绿阔叶灌木,具有较高的保护和园林绿化价值.为了解水杨酸对沙冬青在盐胁迫下的缓解作用,使用水培方法培养沙冬青幼苗,测定其在w=1.3%NaCl胁迫下经不同浓度水杨酸处理后的抗氧化酶活性、PSⅡ光化学效率及其他与植物抗性有关的生理指标的变化.结果表明,0.5 mmol·L~(-1)水杨酸处理下,根和茎叶鲜质量比盐胁迫组分别提高了100%和85%,总叶绿素含量达到盐胁迫组的1.3倍;而1 mmol·L~(-1)水杨酸可使根和茎叶鲜质量均提高90%,总叶绿素含量达到盐胁迫组的1.7倍.这2种浓度的水杨酸均可使叶绿素荧光参数恢复到正常对照水平.水杨酸可以显著增强超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,其中1.0 mmol·L~(-1)水杨酸处理下叶片与根部SOD酶活性分别比盐胁迫组升高了54%和68%;POD酶活性则分别提高了80%和117%,均显著高于对照组与盐胁迫组.同时,水杨酸还抑制了过氧化氢酶(CAT)的活性,减少丙二醛积累,从而缓解NaCl对沙冬青的胁迫.但是水杨酸浓度过高(达到2.0 mmol·L~(-1)),沙冬青幼苗生长即受到抑制,各项生理指标均低于单纯盐胁迫下的幼苗.综合各项生理指标,水杨酸1 mmol·L~(-1)的施用效果优于0.5 mmol·L~(-1).
빈위식물사동청Ammopiptanthus mongolicus위아주중부황막지구유일적상록활협관목,구유교고적보호화완림녹화개치.위료해수양산대사동청재염협박하적완해작용,사용수배방법배양사동청유묘,측정기재w=1.3%NaCl협박하경불동농도수양산처리후적항양화매활성、PSⅡ광화학효솔급기타여식물항성유관적생리지표적변화.결과표명,0.5 mmol·L~(-1)수양산처리하,근화경협선질량비염협박조분별제고료100%화85%,총협록소함량체도염협박조적1.3배;이1 mmol·L~(-1)수양산가사근화경협선질량균제고90%,총협록소함량체도염협박조적1.7배.저2충농도적수양산균가사협록소형광삼수회복도정상대조수평.수양산가이현저증강초양화물기화매(SOD)화과양화물매(POD)활성,기중1.0 mmol·L~(-1)수양산처리하협편여근부SOD매활성분별비염협박조승고료54%화68%;POD매활성칙분별제고료80%화117%,균현저고우대조조여염협박조.동시,수양산환억제료과양화경매(CAT)적활성,감소병이철적루,종이완해NaCl대사동청적협박.단시수양산농도과고(체도2.0 mmol·L~(-1)),사동청유묘생장즉수도억제,각항생리지표균저우단순염협박하적유묘.종합각항생리지표,수양산1 mmol·L~(-1)적시용효과우우0.5 mmol·L~(-1).
Ammopiptanthus mongolicus, an endangered evergreen leguminous shrub, endemic to the semi-arid desert region of central Asia, has higher protection and landscaping value.In order to understand the mitigative effect of salicylic acid on salt stress of A. mongolicus seedlings, the seedlings were treated with w=1.3% NaCl and different concentrations of salicylic acid (SA) under water culture.Antioxidant enzyme activities, photochemical efficiency of PSⅡ and other related physiological indexes were measured and analyzed.The results showed that 0.5 and 1.0 mmol·L~(-1)SA increased the fresh mass, the chlorophyll content and the Chl a/Chl b ratio, recovered fluorescent parameters (F_v/F_0,F_v/F_m and F_0) to normal level, enhanced SOD and POD activity obviously, inhibited CAT activity, and decreased MDA content.When the concentration reached 2.0 mmol·L~(-1), the seedlings growth were inhibited, every physiological indexes of the group were obviously lower than w=1.3% NaCl groups.The application effect of 1 mmol·L~(-1)SA was better than 0.5 mmol·L~(-1).