湖北农业科学
湖北農業科學
호북농업과학
2010年
2期
424-426
,共3页
孙良顺%郭勇全%申金龙%李峰%苏巧%唐志华
孫良順%郭勇全%申金龍%李峰%囌巧%唐誌華
손량순%곽용전%신금룡%리봉%소교%당지화
川贝%生物碱%超声波%正交试验
川貝%生物堿%超聲波%正交試驗
천패%생물감%초성파%정교시험
Fritillaria cirrhosa%alkaloid%ultrasonic wave%orthogonal experiment.
为优化川贝中生物碱的提取工艺,采用超声辅助酸性醇提取法,选取pH值、乙醇体积分数、超声时间、料液比作为考察条件进行单因素试验,并进行L_9(3~4)正交试验.结果表明,pH值、乙醇体积分数对生物碱提取的影响较大.川贝中总生物碱的最优提取组合为A_1B_2C_3D_1,即pH值为1,乙醇体积分数50%,超声时间75 min,料液比1:10.
為優化川貝中生物堿的提取工藝,採用超聲輔助痠性醇提取法,選取pH值、乙醇體積分數、超聲時間、料液比作為攷察條件進行單因素試驗,併進行L_9(3~4)正交試驗.結果錶明,pH值、乙醇體積分數對生物堿提取的影響較大.川貝中總生物堿的最優提取組閤為A_1B_2C_3D_1,即pH值為1,乙醇體積分數50%,超聲時間75 min,料液比1:10.
위우화천패중생물감적제취공예,채용초성보조산성순제취법,선취pH치、을순체적분수、초성시간、료액비작위고찰조건진행단인소시험,병진행L_9(3~4)정교시험.결과표명,pH치、을순체적분수대생물감제취적영향교대.천패중총생물감적최우제취조합위A_1B_2C_3D_1,즉pH치위1,을순체적분수50%,초성시간75 min,료액비1:10.
The optimization of the conditions for extractions of total alkaloids and aconitine from Fritillaria cirrhosa was studied by using the ultrasonic wave irradiation extraction and acidity alcohol.The pH,alcohol concentration,time of ultrasonic wave irradiation and the ratio of material liquid were selected as the inspection conditions in single factor experiment.At the same time the L_9(3~4) orthogonal experiment was carried out.The result indicated that the pH and alcohol concentration were the mainly influential factors for the extraction of alkaloids.Analysis of the orthogonal experiment indicated that the best conditions were A_1B_2C_3D_1,i.e.,pH=1,alcohol concentration 50%,time of ultrasonic wave irradiation 75 min,the ratio of material to liquid 1/10.
