CAJ | 학술논문

目的比较Astoria-Pacific流式荧光分析法与Perking-Elmer时间分辨荧光分析法在新生儿苯丙酮尿症(PKU)筛查工作中的应用价值.方法分别采用Astoria-Pacific流式荧光法(AP流式荧光法)和WALLAC的Perking-Elmer时间分辨荧光分析法(PE荧光法)同时对1 103例健康新生儿的筛查血片和27例已经确诊的PKU阳性患儿血片进行筛查.收集试验数据分析比较两种方法的相关性及有效性.同时应用AP流式荧光法对不同浓度的已知样本进行检测,统计分析这些检测数据后对AP流式荧光法进行方法学的评价.结果两种方法有很好的相关性(r=0.975),同时,AP流式荧光法的阳性复合率(103.7%)和阴性复合率(99.9%)均较PE荧光法高.AP流式荧光法的准确度、精密度、携带率及最低检测限均能胜任PKU的筛查工作,其中AP流式荧光法的Phe/Tyr比值是提高PKU筛查准确度的重要参数.结论 AP流式荧光法优于PE荧光法,完全适合新生儿PKU的筛查工作,值得推广应用.
목적 비교Astoria-Pacific류식형광분석법여Perking-Elmer시간분변형광분석법재신생인분병동뇨증(PKU)사사공작중적응용개치.방법 분별채용Astoria-Pacific류식형광법(AP류식형광법)화WALLAC적Perking-Elmer시간분변형광분석법(PE형광법)동시대1 103례건강신생인적사사혈편화27례이경학진적PKU양성환인혈편진행사사.수집시험수거분석비교량충방법적상관성급유효성.동시응용AP류식형광법대불동농도적이지양본진행검측,통계분석저사검측수거후대AP류식형광법진행방법학적평개.결과 량충방법유흔호적상관성(r=0.975),동시,AP류식형광법적양성복합솔(103.7%)화음성복합솔(99.9%)균교PE형광법고.AP류식형광법적준학도、정밀도、휴대솔급최저검측한균능성임PKU적사사공작,기중AP류식형광법적Phe/Tyr비치시제고PKU사사준학도적중요삼수.결론 AP류식형광법우우PE형광법,완전괄합신생인PKU적사사공작,치득추엄응용.
Objective To compare the applications of flow fluorescence cytometry and time resolved fluorescence in screening the phenylketonuria (PKU) among the neonatal. Methods 1103 specimens from newborn and 27 cases with PKU positive which have been made definite diagnosis , were brought into research, all the sample were analyzed with the API 300 SPOTCHECK analyzer ( Astoria-Pacific International,Clackamas Oregon) and VICTOR~② D 1420 analyzer ( Perking-Elmer,Wallac) respectively. We evaluated the continue flow fluorencens analyzer in newborn screening by comparing the validity of the two methods. Results The two methods have good correlation (r=0. 975), As to the continue flow fluorescence method,the positive rate and negetiue rate are better than PE method, the coutinue flow fluorecences method exhibits stable and reproducible operating characteristics. It was suitable for screening , and the ratio(Phe/Tyr) is an important parameters enhanceing the capability of flow fluorescence cytometry. Conclusion Astoria-Pacific's continue flow fluorecense analyzer is better than PE method. It is suitable for screening the PKU in neonatal.