国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2011年
1期
1-6
,共6页
刘娟娟%殷国荣%刘红丽%孟晓丽
劉娟娟%慇國榮%劉紅麗%孟曉麗
류연연%은국영%류홍려%맹효려
刚地弓形虫%排泄-分泌抗原%可溶性速殖子抗原%鼻内免疫%免疫原性
剛地弓形蟲%排洩-分泌抗原%可溶性速殖子抗原%鼻內免疫%免疫原性
강지궁형충%배설-분비항원%가용성속식자항원%비내면역%면역원성
Toxoplasma gondii%Excreted/secreted antigen%Soluble tachyzoite antigen%Intranasal immunization%Immunogenicity
目的 观察刚地弓形虫速殖子排泄-分泌抗原(excreted/secreted antigen,ESA)和可溶性速殖子抗原(soluble tachyzoite antigen,STAg)鼻内免疫小鼠的免疫原性.方法 BALB/c小鼠随机分为3组,每组10只,分别用PBS 20μl/只、ESA和STAg各20μg/只鼻内免疫2次,间隔14 d.分别于末次免疫后14 d每组处死小鼠,计数肠上皮内淋巴细胞(intestinal intraepithelial lymphocytes,iIEL)和脾淋巴细胞,ELISA法检测血清IgG和小肠冲洗液IgA抗体水平.结果 实验期间,末次免疫后14 d,各抗原组脾淋巴细胞及iIEL均增殖活跃,细胞数与PBS组比较,差异具有统计学意义(P＜0.05).各抗原组血清IgG水平在免疫后14 d明显增高,与PBS组比较差异有统计学意义(P＜0.01).免疫后14 d肠液IgA水平ESA和STAg组与PBS组比较差异有统计学意义(P＜0.01).结论 ESA和STAg鼻内免疫均可诱导黏膜及系统的细胞和体液免疫应答,有较强的免疫原性.
目的 觀察剛地弓形蟲速殖子排洩-分泌抗原(excreted/secreted antigen,ESA)和可溶性速殖子抗原(soluble tachyzoite antigen,STAg)鼻內免疫小鼠的免疫原性.方法 BALB/c小鼠隨機分為3組,每組10隻,分彆用PBS 20μl/隻、ESA和STAg各20μg/隻鼻內免疫2次,間隔14 d.分彆于末次免疫後14 d每組處死小鼠,計數腸上皮內淋巴細胞(intestinal intraepithelial lymphocytes,iIEL)和脾淋巴細胞,ELISA法檢測血清IgG和小腸遲洗液IgA抗體水平.結果 實驗期間,末次免疫後14 d,各抗原組脾淋巴細胞及iIEL均增殖活躍,細胞數與PBS組比較,差異具有統計學意義(P＜0.05).各抗原組血清IgG水平在免疫後14 d明顯增高,與PBS組比較差異有統計學意義(P＜0.01).免疫後14 d腸液IgA水平ESA和STAg組與PBS組比較差異有統計學意義(P＜0.01).結論 ESA和STAg鼻內免疫均可誘導黏膜及繫統的細胞和體液免疫應答,有較彊的免疫原性.
목적 관찰강지궁형충속식자배설-분비항원(excreted/secreted antigen,ESA)화가용성속식자항원(soluble tachyzoite antigen,STAg)비내면역소서적면역원성.방법 BALB/c소서수궤분위3조,매조10지,분별용PBS 20μl/지、ESA화STAg각20μg/지비내면역2차,간격14 d.분별우말차면역후14 d매조처사소서,계수장상피내림파세포(intestinal intraepithelial lymphocytes,iIEL)화비림파세포,ELISA법검측혈청IgG화소장충세액IgA항체수평.결과 실험기간,말차면역후14 d,각항원조비림파세포급iIEL균증식활약,세포수여PBS조비교,차이구유통계학의의(P＜0.05).각항원조혈청IgG수평재면역후14 d명현증고,여PBS조비교차이유통계학의의(P＜0.01).면역후14 d장액IgA수평ESA화STAg조여PBS조비교차이유통계학의의(P＜0.01).결론 ESA화STAg비내면역균가유도점막급계통적세포화체액면역응답,유교강적면역원성.
Objective To study the immunogenicity of Toxoplasma gondii excreted/secreted antigens (ESA) and soluble tachyzoite antigen (STAg) after intranasal immunization. Methods BALB/c mice were randomly divided into 3 groups, 10 mice each group. Mice were intranasally immunized with 20 μl PBS per mouse and 20 μg ESA or STAg per mouse, respectively, twice at an internal of 2 weeks. Ten mice of each group were killed on day 14 after the last immunization. Spleen lymphocytes and intestinal intraepithelial lymphocytes (iIEL) were counted. Serum IgG and IgA in intestinal washes were detected by ELISA. Results Cell proliferation of spleen lymphocytes and iIEL was observed in all antigen groups on day 14 after the last immunization, and compared with the PBS group, there was a significant difference ( P ＜0.05). The level of serum IgG in all antigen groups was higher than that in PBS group on day 14 after immunization with a significant difference ( P ＜0.01 ). On day 14 after immunization, the level of IgA in ESA group and STAg group was higher than that in PBS group (P ＜ 0.01 ). Conclusion Intranasal immunized with ESA or STAg can effectively induce the mucosal and systemic immune responses with potent immunogenicity.
