中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
1期
47-51
,共5页
吴惠%王德文%王水明%左红艳%李杨%高亚兵%徐新萍%周红梅%苏镇涛%姚华%李宁%彭瑞云
吳惠%王德文%王水明%左紅豔%李楊%高亞兵%徐新萍%週紅梅%囌鎮濤%姚華%李寧%彭瑞雲
오혜%왕덕문%왕수명%좌홍염%리양%고아병%서신평%주홍매%소진도%요화%리저%팽서운
电磁辐射%Sertoli细胞%SOX9%威尔姆氏瘤核蛋白1
電磁輻射%Sertoli細胞%SOX9%威爾姆氏瘤覈蛋白1
전자복사%Sertoli세포%SOX9%위이모씨류핵단백1
Electromagnetic radiation%Sertoli cells%SOX9%WT1
目的 探讨不同波段电磁辐射对大鼠睾丸支持细胞(Sertoli细胞)性别决定相关基因SOX9和WT1表达的影响.方法 原代分离3周Wistar大鼠的Sertoli细胞,应用WT1免疫细胞化学染色鉴定细胞纯度；Sertoli细胞经平均功率密度为100 mW/cm2的S波段微波和X波段微波及场强6×104 V/m的电磁脉冲辐射4 min.应用实时RT-PCR和Western blot方法,检测SOX9、WT1的mRNA和蛋白质的变化.结果 3种波段电磁辐射后Sertoli细胞SOX9 mRNA和蛋白表达在辐射后6和12 h及辐射后的6和24 h均见不同程度的降低(F=15.20和4.84,P＜0.05;F =8.46和7.47,P ＜0.05)；WT1 mRNA和蛋白表达在辐射后6和12 h及辐射后的24 h出现不同程度降低(F=13.46、5.08和10.26,P＜0.05).结论 3种波段电磁辐射后Sertoli细胞性别决定相关基因(SOX9、WT1)表达呈不同程度的降低.Sertoli细胞SOX9、WT1的变化可能参与了电磁辐射致生殖危害的病理生理过程.
目的 探討不同波段電磁輻射對大鼠睪汍支持細胞(Sertoli細胞)性彆決定相關基因SOX9和WT1錶達的影響.方法 原代分離3週Wistar大鼠的Sertoli細胞,應用WT1免疫細胞化學染色鑒定細胞純度；Sertoli細胞經平均功率密度為100 mW/cm2的S波段微波和X波段微波及場彊6×104 V/m的電磁脈遲輻射4 min.應用實時RT-PCR和Western blot方法,檢測SOX9、WT1的mRNA和蛋白質的變化.結果 3種波段電磁輻射後Sertoli細胞SOX9 mRNA和蛋白錶達在輻射後6和12 h及輻射後的6和24 h均見不同程度的降低(F=15.20和4.84,P＜0.05;F =8.46和7.47,P ＜0.05)；WT1 mRNA和蛋白錶達在輻射後6和12 h及輻射後的24 h齣現不同程度降低(F=13.46、5.08和10.26,P＜0.05).結論 3種波段電磁輻射後Sertoli細胞性彆決定相關基因(SOX9、WT1)錶達呈不同程度的降低.Sertoli細胞SOX9、WT1的變化可能參與瞭電磁輻射緻生殖危害的病理生理過程.
목적 탐토불동파단전자복사대대서고환지지세포(Sertoli세포)성별결정상관기인SOX9화WT1표체적영향.방법 원대분리3주Wistar대서적Sertoli세포,응용WT1면역세포화학염색감정세포순도；Sertoli세포경평균공솔밀도위100 mW/cm2적S파단미파화X파단미파급장강6×104 V/m적전자맥충복사4 min.응용실시RT-PCR화Western blot방법,검측SOX9、WT1적mRNA화단백질적변화.결과 3충파단전자복사후Sertoli세포SOX9 mRNA화단백표체재복사후6화12 h급복사후적6화24 h균견불동정도적강저(F=15.20화4.84,P＜0.05;F =8.46화7.47,P ＜0.05)；WT1 mRNA화단백표체재복사후6화12 h급복사후적24 h출현불동정도강저(F=13.46、5.08화10.26,P＜0.05).결론 3충파단전자복사후Sertoli세포성별결정상관기인(SOX9、WT1)표체정불동정도적강저.Sertoli세포SOX9、WT1적변화가능삼여료전자복사치생식위해적병리생리과정.
Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP ( electromagnetic pulse),S-HPM ( S-band high power microwave) and X-HPM ( Xband high power microwave).Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1.After exposure to 6 × 104 V/m EMP,100 mW/cm2 S-HPM and X-HPM for 4 min respectively,SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot,respectively.Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave ( F =15.20and 4.84,P ＜ 0.05 ).SOX9 protein expression was also decreased at 6 and 24 h after irradiation ( F =8.46 and 7.47,P＜0.05).WT1 mRNA expression was decreased at6 and 12 h (F=13.46 and 5.08,P ＜ 0.05 ),but its protein expression was decreased only at 24 h post-irradiation ( F =10.26,P ＜ 0.05 ).Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells,which may provide molecular foundation for genital system hazards induced by microwave radiation.
