中国医药
中國醫藥
중국의약
CHINA MEDICINE
2010年
4期
295-297
,共3页
祁莉萍%严晓伟%叶平%党爱民
祁莉萍%嚴曉偉%葉平%黨愛民
기리평%엄효위%협평%당애민
冠状动脉粥样硬化性心脏病%三磷酸腺苷结合盒转运子A1%胆固醇逆向转运%单核苷酸多态性
冠狀動脈粥樣硬化性心髒病%三燐痠腺苷結閤盒轉運子A1%膽固醇逆嚮轉運%單覈苷痠多態性
관상동맥죽양경화성심장병%삼린산선감결합합전운자A1%담고순역향전운%단핵감산다태성
Coronary heart disease%ATP-binding cassette A1%Reverse cholesterol transport%Single nucleotide polymorphisms
目的 首次研究汉族人群冠心病合并糖尿病与三磷酸腺苷结合盒转运子A1(ABCA1)基因启动子区-565C/T及7外显子R219K基因多态性关联分析.方法 应用连接酶检测反应法对172例合并糖尿病冠心病患者及393例对照组测试-565C/T及R219K基因型.结果 合并糖尿病冠心病患者-565C/T位点CC、CT及,TT基因型频率分别为0.360(n=63),0.482(n=83),0.157(n=27),与对照相比,TT基因型及T等位基因频率分别为0.157 vs 0.163,0.398 vs 0.409(均P>0.05).R219K位点从+AG基因型合并糖尿病的冠心病组与对照组分别为0.65 vs 0.73,P=0.079.关联分析显示,AA基因型系冠心病保护性因素,[OR=0.428(95%CI 0.227～0.603),P=0.009].结论 ABCA1基因-565C/T位点T等位基因与合并糖尿病的冠心病无关联,R219K的A等位基因在合并糖尿病的冠心病组频率较低,提示A等位基因系冠心病保护性因子.
目的 首次研究漢族人群冠心病閤併糖尿病與三燐痠腺苷結閤盒轉運子A1(ABCA1)基因啟動子區-565C/T及7外顯子R219K基因多態性關聯分析.方法 應用連接酶檢測反應法對172例閤併糖尿病冠心病患者及393例對照組測試-565C/T及R219K基因型.結果 閤併糖尿病冠心病患者-565C/T位點CC、CT及,TT基因型頻率分彆為0.360(n=63),0.482(n=83),0.157(n=27),與對照相比,TT基因型及T等位基因頻率分彆為0.157 vs 0.163,0.398 vs 0.409(均P>0.05).R219K位點從+AG基因型閤併糖尿病的冠心病組與對照組分彆為0.65 vs 0.73,P=0.079.關聯分析顯示,AA基因型繫冠心病保護性因素,[OR=0.428(95%CI 0.227～0.603),P=0.009].結論 ABCA1基因-565C/T位點T等位基因與閤併糖尿病的冠心病無關聯,R219K的A等位基因在閤併糖尿病的冠心病組頻率較低,提示A等位基因繫冠心病保護性因子.
목적 수차연구한족인군관심병합병당뇨병여삼린산선감결합합전운자A1(ABCA1)기인계동자구-565C/T급7외현자R219K기인다태성관련분석.방법 응용련접매검측반응법대172례합병당뇨병관심병환자급393례대조조측시-565C/T급R219K기인형.결과 합병당뇨병관심병환자-565C/T위점CC、CT급,TT기인형빈솔분별위0.360(n=63),0.482(n=83),0.157(n=27),여대조상비,TT기인형급T등위기인빈솔분별위0.157 vs 0.163,0.398 vs 0.409(균P>0.05).R219K위점종+AG기인형합병당뇨병적관심병조여대조조분별위0.65 vs 0.73,P=0.079.관련분석현시,AA기인형계관심병보호성인소,[OR=0.428(95%CI 0.227～0.603),P=0.009].결론 ABCA1기인-565C/T위점T등위기인여합병당뇨병적관심병무관련,R219K적A등위기인재합병당뇨병적관심병조빈솔교저,제시A등위기인계관심병보호성인자.
Objective The promoter-565C/T variant and the 7exon R219K variant are associated with risk of Coronary heart disease (CAD), but the association also remains controversial. At present, there are few studies focusing on the associations between ATP-binding cassette A1 (ABCA1), and CAD with Diabetes mellitus (DM) in Chinese population. Since decreased serum level of HDL-C is often observed in DM,it is natural to hypothesize that polymorphisms of the ABCA1 gene might be related to CAD complicated with DM. Objective To study the mutations and genetic characteristics of ABCA1 promoter -565C/T and 7Exon R219K in CAD with DM patients in Chinese Han people. Methods One hundred and seventy-three patients of CAD with DM and 389 controls were genotyped for-565C/T, R219K used with LDR. Genetic association analysis was performed. Results The frequencies of the CC, CT, and TT genotypes in CAD with DM were 0.360(n=63), 0.482 (n=83) and 0.157 (n=27), respectively. The frequency of the TT genotype and T allele at the-565C/T locus had no significant alterations between CAD with DM patients and Controls (0.157 vs 0.163; 0.398 vs 0.409,P>0.05). The frequency of the AA and GA geno-type at the R219K locus was lower in CAD patients compared with diabetes (0.65 vs 0.73,P=0.079). Logistic re-gression model were performed, revealed no interaction between 2 SNPs and traditional risk factors, but R219K had a protection effect, OR=0.428 (95%CI 0.227-0.603), P=0.009. Conclusions ABCA1 the T allele of-565 C/T SNP has no significant association with CAD with DM. R219K SNP predicts differences in CAD with diabetes. The AA genotype may protect against subclinical cardiovascular disease.
