CAJ | 학술논문

目的观察雷帕霉素对人胃癌细胞株SGC-7901和MKN45的增殖、凋亡以及侵袭能力的影响并探讨其机制.方法采用不同浓度的雷帕霉素(5、10、20、40 nmol/L),噻唑蓝(MTT)和流式细胞术分别检测SGC-7901和MKN45细胞的增殖和凋亡、Transwell检测胃癌细胞的侵袭能力、逆转录-聚合酶链反应( RT-PCR)和Western blot检测Survivin和mTOR的mRNA和蛋白的表达.结果不同浓度的雷帕霉素可以抑制胃癌细胞株SGC-7901和MKN45的增殖并诱导其发生凋亡,同时可降低其体外侵袭能力,其作用具有剂量依赖性.RT-PCR和Western blot检测显示胃癌细胞中mTOR和Survivin的mRNA和蛋白表达在雷帕霉素作用后受到抑制.结论雷帕霉素可以体外抑制胃癌细胞的增殖和侵袭并且诱导其发生凋亡,其可能是通过抑制mTOR和Survivin的表达来发挥作用的.
목적 관찰뢰파매소대인위암세포주SGC-7901화MKN45적증식、조망이급침습능력적영향병탐토기궤제.방법 채용불동농도적뢰파매소(5、10、20、40 nmol/L),새서람(MTT)화류식세포술분별검측SGC-7901화MKN45세포적증식화조망、Transwell검측위암세포적침습능력、역전록-취합매련반응( RT-PCR)화Western blot검측Survivin화mTOR적mRNA화단백적표체.결과 불동농도적뢰파매소가이억제위암세포주SGC-7901화MKN45적증식병유도기발생조망,동시가강저기체외침습능력,기작용구유제량의뢰성.RT-PCR화Western blot검측현시위암세포중mTOR화Survivin적mRNA화단백표체재뢰파매소작용후수도억제.결론 뢰파매소가이체외억제위암세포적증식화침습병차유도기발생조망,기가능시통과억제mTOR화Survivin적표체래발휘작용적.
Objective To observe the effects of rapamycin on proliferation,apoptosis and invasion of human gastric cancer cell lines,SGC-7901 and MKN 45,and its mechanisms.Methods Different concentrations of rapamycin (5,10,20,40 nmol/L) were used.Methyl thiazol tetrazolium (MTT) and flow cytometry assays were used to detect the proliferation and apoptosis of SGC-7901 and MKN 45 cells.Transwell assay was used to observe the invasive ability of gastric cancer cells.By using reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blotting,the mRNA and protein expression of mTOR and Survivin was examined.Results Our data showed that rapamycin could inhibit proliferation and invasive ability of SGC-7901 and MKN 45 cells,and induce apoptosis of these two gastric cancer cell lines in a dose-dependent manner.The mRNA and protein expression of mTOR and Survivin was suppressed by rapamycin.Conclusion Rapamycin could inhibit proliferation,invasive ability and induce apoptosis of human gastric cancer cellsin vitroprobably by inhibiting the expression of mTOR and Survivin.