CAJ | 학술논문

目的观察软骨素酶联合雪旺细胞移植在治疗急性脊髓损伤中的作用.方法 Wistar大鼠80只,制作T10节段急性脊髓损伤模型(致伤力10g×4cm).随机分成4组:对照组、雪旺细胞移植组、硫酸软骨素酶治疗组和联合治疗组.采用脊髓运动功能评分(BBB法,总分21)、神经电生理(SEP&MEP)检查和生物素葡聚糖胺(BDA)神经示踪及标本NF-200免疫组织化学染色等比较各组疗效.结果 4周后BBB评分实验组较对照组明显提高,各组间差异有统计学意义(P<0.05)(12周时4组分别为9.11±1.41、11.22±1.59、11.77±1.76和14.22±1.92).术后4周起,神经电生理检查实验组动物较对照组差异有统计学意义(P<0.05),12周时4组MEP的波幅分别恢复至术前的28.8%、44.9%、49.0%和56.8%.BDA示踪显示联合组较对照组有较多的神经纤维穿过损伤部位.NF-200免疫组织化学染色吸光度(A)比较各组间差异有统计学意义(P<0.05),3个实验组纵切片A值与对照组的比值为1.44、1.55和2.78.结论联合应用软骨素酶和雪旺细胞移植来治疗脊髓损伤,起到协同作用,效果好于单一方法,能明显促进脊髓损伤后的轴突再生和肢体功能恢复.
목적 관찰연골소매연합설왕세포이식재치료급성척수손상중적작용.방법 Wistar대서80지,제작T10절단급성척수손상모형(치상력10g×4cm).수궤분성4조:대조조、설왕세포이식조、류산연골소매치료조화연합치료조.채용척수운동공능평분(BBB법,총분21)、신경전생리(SEP&MEP)검사화생물소포취당알(BDA)신경시종급표본NF-200면역조직화학염색등비교각조료효.결과 4주후BBB평분실험조교대조조명현제고,각조간차이유통계학의의(P<0.05)(12주시4조분별위9.11±1.41、11.22±1.59、11.77±1.76화14.22±1.92).술후4주기,신경전생리검사실험조동물교대조조차이유통계학의의(P<0.05),12주시4조MEP적파폭분별회복지술전적28.8%、44.9%、49.0%화56.8%.BDA시종현시연합조교대조조유교다적신경섬유천과손상부위.NF-200면역조직화학염색흡광도(A)비교각조간차이유통계학의의(P<0.05),3개실험조종절편A치여대조조적비치위1.44、1.55화2.78.결론 연합응용연골소매화설왕세포이식래치료척수손상,기도협동작용,효과호우단일방법,능명현촉진척수손상후적축돌재생화지체공능회복.
Objective To investigate the effect of the combination of chondroitinase and Schwann cells (SCs) transplantation after spinal cord injury (SCI) in adult rats. Methods Eighty adult Wistar rats were divided into four groups; the control group (A), the Schwann cells transplantation group ( B), the chondroitinase ABC(ChABC) group (C) and the SCs transplantation plus chondroitinase ABC group (D). The thoracic T10 spinal cord injury model was made by Impactor model II manual (10g×4 cm). After SCI, the rats in group B received SCs transplantation in injured sites, those in group C received ChABC treatment, and those in group D received the combined treatment. The rats in group A only received blank saline and medium. The locomotor functions were evaluated according to the BBB score scale (total 21), and the neuroelectrophysiological changes (SEP&MEP) were examined by electromyographic machine. Twelve weeks after SCI, biotinylated dextran amine (BDA) was injected into the cerebral cortex to trace the corticospinal tracts. Two weeks after the tracing, the animals were killed and the spinal cord frozen sections were made. The tissue sections were processed with Cy3 fluorescence stain, HE stain and anti NF-200 immuohistological stain. Results From 4 weeks after SCI, the rats in groups B, C and D had obviously higher BBB scores than in control group (P<0.05) , and BBB scores in group D was the highest. The scores in groups A, B, C and D were 9. 11 ±1.41, 11.22 ±1.59, 11.77 ±1.76 and 14. 22 ± 1. 92 respectively 12 weeks later. In electromyographic examination (SEP&MEP) , the rats in groups B, C and D gained better recovery than group A (P <0. 05), and group D was the best. At the week 12, the MEP wave recovery rate was 28. 8% , 44. 9% , 49. 0% and 56. 8% in groups A, B, C and D respectively. In BDA tracing slides, some axons could cross the injured sites in group D. There was significant difference in anti-NF-200 stain area among four groups (P<0.05). The absorbance ( A) ratio between groups B, D, D and group A was 1.44, 1. 55 and 2. 78, respectively. Conclusion Combined therapy with ChABC and SCs transplantation can promote axonal regeneration and function recovery after SCI.