中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
16期
1095-1099
,共5页
史家岚%李金梅%侯金晓%曹峰林%刘述川%周晋%高春艳%王晶%谭小燕%李甲楠%刘玥%刘岩%马瑞爽%杨雪
史傢嵐%李金梅%侯金曉%曹峰林%劉述川%週晉%高春豔%王晶%譚小燕%李甲楠%劉玥%劉巖%馬瑞爽%楊雪
사가람%리금매%후금효%조봉림%류술천%주진%고춘염%왕정%담소연%리갑남%류모%류암%마서상%양설
白血病,早幼粒细胞,急性%砷剂%柔红霉素%磷脂酰丝氨酸类%乳黏素
白血病,早幼粒細胞,急性%砷劑%柔紅黴素%燐脂酰絲氨痠類%乳黏素
백혈병,조유립세포,급성%신제%유홍매소%린지선사안산류%유점소
Leukemia,promyelocytic,acute%Arsenicals%Daunorubicin%Phosphatidylserines%Lactadherin
目的 观察三氧化二砷(ATO)和柔红霉素(DNR)对急性早幼粒细胞白血病(APL)细胞表面磷脂酰丝氨酸(PS)暴露及促凝血活性的影响.方法 提取2007年3月至2009年2月就诊于哈尔滨医科大学附属第一医院的12例初发APL患者的APL细胞,以12份健康志愿者全血中提取的单个核细胞和中性粒细胞作为对照.用1μmol/L ATO、DNR分别处理APL细胞24 h,通过流式细胞术和共聚焦显微镜检测APL细胞的PS暴露,通过凝血时间和凝血因子生成实验测定相关的凝血活性.乳黏素作为检测APL细胞PS暴露的探针和凝血抑制剂.结果 流式细胞仪和共聚焦显微镜结果显示,与未处理组比较,ATO处理组APL细胞PS暴露减少；而DNR处理组APL细胞PS暴露明显增加.ATO处理组,APL细胞的凝血时间比未处理组长[(220±41)s比(180±25)s,P＜0.05],凝血因子生成比未处理组少(均P＜0.05)；而DNR处理组,APL细胞的凝血时间比未处理组短[(80±20)s比(180±25)s,P＜0.05],凝血因子生成比未处理组多(均P＜0.05).加入乳黏素后,DNR处理组APL细胞的促凝活性降低[内源性、外源性凝血因子Xa及凝血酶生成均减少(均P＜0.05)].结论 APL细胞的PS暴露量与其促凝活性呈正相关.ATO通过减少APL细胞表面的PS暴露而减弱其促凝活性,DNR通过增加APL细胞表面PS暴露而增强其促凝血活性.
目的 觀察三氧化二砷(ATO)和柔紅黴素(DNR)對急性早幼粒細胞白血病(APL)細胞錶麵燐脂酰絲氨痠(PS)暴露及促凝血活性的影響.方法 提取2007年3月至2009年2月就診于哈爾濱醫科大學附屬第一醫院的12例初髮APL患者的APL細胞,以12份健康誌願者全血中提取的單箇覈細胞和中性粒細胞作為對照.用1μmol/L ATO、DNR分彆處理APL細胞24 h,通過流式細胞術和共聚焦顯微鏡檢測APL細胞的PS暴露,通過凝血時間和凝血因子生成實驗測定相關的凝血活性.乳黏素作為檢測APL細胞PS暴露的探針和凝血抑製劑.結果 流式細胞儀和共聚焦顯微鏡結果顯示,與未處理組比較,ATO處理組APL細胞PS暴露減少；而DNR處理組APL細胞PS暴露明顯增加.ATO處理組,APL細胞的凝血時間比未處理組長[(220±41)s比(180±25)s,P＜0.05],凝血因子生成比未處理組少(均P＜0.05)；而DNR處理組,APL細胞的凝血時間比未處理組短[(80±20)s比(180±25)s,P＜0.05],凝血因子生成比未處理組多(均P＜0.05).加入乳黏素後,DNR處理組APL細胞的促凝活性降低[內源性、外源性凝血因子Xa及凝血酶生成均減少(均P＜0.05)].結論 APL細胞的PS暴露量與其促凝活性呈正相關.ATO通過減少APL細胞錶麵的PS暴露而減弱其促凝活性,DNR通過增加APL細胞錶麵PS暴露而增彊其促凝血活性.
목적 관찰삼양화이신(ATO)화유홍매소(DNR)대급성조유립세포백혈병(APL)세포표면린지선사안산(PS)폭로급촉응혈활성적영향.방법 제취2007년3월지2009년2월취진우합이빈의과대학부속제일의원적12례초발APL환자적APL세포,이12빈건강지원자전혈중제취적단개핵세포화중성립세포작위대조.용1μmol/L ATO、DNR분별처리APL세포24 h,통과류식세포술화공취초현미경검측APL세포적PS폭로,통과응혈시간화응혈인자생성실험측정상관적응혈활성.유점소작위검측APL세포PS폭로적탐침화응혈억제제.결과 류식세포의화공취초현미경결과현시,여미처리조비교,ATO처리조APL세포PS폭로감소；이DNR처리조APL세포PS폭로명현증가.ATO처리조,APL세포적응혈시간비미처리조장[(220±41)s비(180±25)s,P＜0.05],응혈인자생성비미처리조소(균P＜0.05)；이DNR처리조,APL세포적응혈시간비미처리조단[(80±20)s비(180±25)s,P＜0.05],응혈인자생성비미처리조다(균P＜0.05).가입유점소후,DNR처리조APL세포적촉응활성강저[내원성、외원성응혈인자Xa급응혈매생성균감소(균P＜0.05)].결론 APL세포적PS폭로량여기촉응활성정정상관.ATO통과감소APL세포표면적PS폭로이감약기촉응활성,DNR통과증가APL세포표면PS폭로이증강기촉응혈활성.
Objective To explore the effect of arsenic trioxide (ATO) and daunorubicin (DNR)on phosphatidylserine (PS) exposure and related procoagulant activity of acute promyelocytic leukemia (APL) cells.Methods Mononuclear cell and neutrophil isolated from whole blood of 12 healthy volunteers were used as control group while APL cells obtained from 12 newly diagnosed APL patients at First Affiliated Hospital,Harbin Medical University from March 2007 to February 2009 were used as experimental group.APL cells were treated with 1 μmol/L ATO and 1 μmol/L DNR for 24 h.PS exposure of APL cells were measured by flow cytometry and confocal microscopy.And the related procoagulant activity was detected by the assays of coagulation time and coagulation factor formation.Lactadherin was used as a probe for PS exposure and anticoagulant on the cells of 12 APL patients. Results ATO induced a decrease of PS exposure on APL cells by flow cytometry and no staining with lactadherin was observed under confocal microscopy.However,DNR induced the significantly elevated PS exposure and staining green with a rim pattern on membrane of APL cells was obtained.Coagulation time was ( 180 ± 25 ) s and (220 ± 41 ) s before and after treatment with ATO,respectively ( P ＜ 0.05 ). The formation of coagulation factors decreased after treatment with ATO ( P ＜ 0.05 ).While coagulation time was ( 180 ± 25 ) s and (80 ± 20) s before and after treatment with DNR,respectively ( P ＜ 0.05 ). The formation of coagulation factors increased after treatment with DNR ( all P ＜ 0.05 ).Lactadherin inhibited the procoagulant activities of DNR-treated APL cells (all P ＜0.05 ).Conclusions Procoagulant activity is positively correlated with the exposed PS of APL cells.ATO and DNR inhibited and enhanced procoagulant activity with decreased and increased PS exposure,respectively.
