CAJ | 학술논문

目的探讨微生物对青蒿素(Ⅰ)的转化作用.方法通过华根霉和雅致小克银汉霉在土豆培养基中发酵产酶,对青蒿素进行转化.结果两株菌对底物均有转化作用,分离出去氧青蒿素deoxyartemisinin(Ⅱ),3α-羟基去氧青蒿素3α-hydroxydeoxyartemisinin(Ⅲ)和9β-羟基青蒿素9β-hydroxyartemisinin(Ⅳ)共3个产物,其中Ⅳ为一新化合物,同时振荡条件下底物在无菌培养基中也能发生微量转化得到产物Ⅱ.结论青蒿素易被实验两株真菌转化,同时过氧桥也易断裂而失去一个氧原子成为去氧青蒿素,丧失抗疟活性,起作用的可能是土豆培养基中的铁元素.
목적탐토미생물대청호소(Ⅰ)적전화작용.방법통과화근매화아치소극은한매재토두배양기중발효산매,대청호소진행전화.결과량주균대저물균유전화작용,분리출거양청호소deoxyartemisinin(Ⅱ),3α-간기거양청호소3α-hydroxydeoxyartemisinin(Ⅲ)화9β-간기청호소9β-hydroxyartemisinin(Ⅳ)공3개산물,기중Ⅳ위일신화합물,동시진탕조건하저물재무균배양기중야능발생미량전화득도산물Ⅱ.결론청호소역피실험량주진균전화,동시과양교야역단렬이실거일개양원자성위거양청호소,상실항학활성,기작용적가능시토두배양기중적철원소.
Object To study the microbial transformation of antimalarial drug artemisinin ( I ).Methods The enzymes secreted by fermentation of Rhizopus chinensis Saito CICC 3043 and Cunninghamel-la elegans Lendn. AS 3. 1207 in the potato medium were employed to transform artemisinin. ResultsThree products were obtained, among which deoxyartemisinin ( Ⅱ ) was also found in the controls withoutmicroorganisms. The other two were identified as 3α-hydroxydeoxyartemisinin (Ⅲ) and 9β-hydrox-yartemisinin (Ⅳ). Compound (Ⅳ) was identified as a new one. Conclusion Artemisinin could be trans-formed by the two title strains of microorganisms. It is easy to release one oxygen atom by breaking of perexide bridge in potato medium and to form deoxyartemisinin, thus making it lose its antimalarial activi-ty. The iron (Fe) in the potato medium may have the above function.