CAJ | 학술논문

用焦锑酸钾沉淀法研究了水稻红莲-粤泰不育系及其保持系花药发育过程中Ca2+的分布变化。结果表明：二核期前后，可育花粉和败育花粉表面均积累较多Ca2+的沉淀颗粒；可育花粉内充满浓密细胞质和淀粉粒，无Ca2+沉淀颗粒分布；败育花粉在其细胞质的液泡中积累大量Ca2+沉淀颗粒，胞质中出现液泡化网状结构，并逐步解体成空泡。保持系药隔维管束及薄壁细胞中几乎无Ca2+沉淀颗粒分布；而不育系药隔维管束鞘及其周边细胞、导管与筛管的内壁和液泡中均有较多Ca2+沉淀颗粒分布，薄壁细胞的核分布着较少Ca2+沉淀颗粒。并对不育系花粉内和药隔组织细胞中Ca2+大量积累的异常现象作了深入的探讨。
용초제산갑침정법연구료수도홍련-월태불육계급기보지계화약발육과정중Ca2+적분포변화。결과표명：이핵기전후，가육화분화패육화분표면균적루교다Ca2+적침정과립；가육화분내충만농밀세포질화정분립，무Ca2+침정과립분포；패육화분재기세포질적액포중적루대량Ca2+침정과립，포질중출현액포화망상결구，병축보해체성공포。보지계약격유관속급박벽세포중궤호무Ca2+침정과립분포；이불육계약격유관속초급기주변세포、도관여사관적내벽화액포중균유교다Ca2+침정과립분포，박벽세포적핵분포착교소Ca2+침정과립。병대불육계화분내화약격조직세포중Ca2+대량적루적이상현상작료심입적탐토。
Potassium antimonate was used to locate Ca2+ in fertile and sterile anthers of Honglian-Yuetai cytoplasmic male-sterile rice(Oryza sativa L.). During the development of fertile and sterile anthers, abundant calcium precipitates accumulated on the outer surface of pollen walls at the binucleate stage while starch grains accumulated in pollen. There were no C2+a precipitates in the cytoplasm of fertile pollen grains. However, a great quantity of calcium precipitates accumulated in central vacuole of abortive pollen. There was not distribution of calcium precipitates in connective tissue of fertile anthers. Alternatively, in connective tissue of sterile anthers, abundant calcium precipitates located in vacuole and cytoplasm of vascular bundle sheath cells and its peripheral cells, vessels and sieve tubes, but few calcium precipitates in nuclei of parenchymatous cells. The changes of antimonate-induced calcium precipitates in the pollen and connective tissue of cytoplasmic male-sterile rice indicate that the anomalies in the distribution of calcium accumulation correlate with pollen abortion.