中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
7期
204-205
,共2页
关节炎,类风湿%NF-κB%青风藤%滑膜/细胞学%肿瘤坏死因子
關節炎,類風濕%NF-κB%青風籐%滑膜/細胞學%腫瘤壞死因子
관절염,류풍습%NF-κB%청풍등%활막/세포학%종류배사인자
背景:青藤碱是从中药青风藤中提取的生物碱单体,在治疗类风湿关节炎(rheumatoid arthritis,RA)方面,具有较明确的疗效,但其治疗机制尚不清楚.目的:观察不同剂量的青藤碱体外作用对佐剂性关节炎(adjuvant arthritis,AA)大鼠滑膜细胞核因子-κB(NF-κB)活性及肿瘤坏死因子(TNF-αmRNA、白细胞介素1β(IL-1β)mRNA、白细胞介素10(IL-10)mRNA的影响,以阐明该药治疗RA的可能机制.设计:以细胞为研究对象,分组对照的重复测量设计.单位:一所军医大学医院的中医科和烧伤研究所.材料:实验于2002-03/2002-10在全军烧伤研究所实验室完成.实验动物为健康清洁级雄性Wistar大鼠25只.以AA大鼠为模型,收集滑膜细胞,依次作如下分组:正常对照组,AA组,AA+青藤碱30 mg/L组,AA+青藤碱60 mg/L组,AA+青藤碱120 mg/L组.电泳迁移率改变分析法测NF-κB的活性,反转录PCR检测TNF-α mRNA,IL-1β mRNA,IL-10 mRNA的表达.主要观察指标:不同剂量的青藤碱体外作用对后佐剂性关节炎大鼠滑膜细胞NF-κB活性,TNF-1β mRNA,IL-1β mRNA及IL-10 mRNA的对比结果.结果:与正常对照组相比,AA后滑膜细胞内NF-κB活性与TNF-α mR-NA,IL-1β mRNA,IL-10 mRNA的表达均显著升高,分别为17±6,0.570±0.047,0.730±0.093,0.683±0.081(t=2.71～4.07,P<0.05).经青藤碱作用后,NF-κB活性的变化趋势与TNF-α mRNA,IL-1 mRNA的相关性较好(r=0.810,P<0.001;r=0.562,P<0.05),与IL-10 mRNA无统计学上的相关性,青藤碱在一定的浓度范围(30～120 mg/L)内呈浓度依赖性抑制NF-κB活性与TNF-α mR-NA,IL-1β mRNA的表达,而对IL-10 mRNA的抑制效应与浓度无关.结论:青藤碱可能通过抑制NF-κB活性而降低滑膜细胞内TNF-α mR-NA及IL-1β mRNA的表达.
揹景:青籐堿是從中藥青風籐中提取的生物堿單體,在治療類風濕關節炎(rheumatoid arthritis,RA)方麵,具有較明確的療效,但其治療機製尚不清楚.目的:觀察不同劑量的青籐堿體外作用對佐劑性關節炎(adjuvant arthritis,AA)大鼠滑膜細胞覈因子-κB(NF-κB)活性及腫瘤壞死因子(TNF-αmRNA、白細胞介素1β(IL-1β)mRNA、白細胞介素10(IL-10)mRNA的影響,以闡明該藥治療RA的可能機製.設計:以細胞為研究對象,分組對照的重複測量設計.單位:一所軍醫大學醫院的中醫科和燒傷研究所.材料:實驗于2002-03/2002-10在全軍燒傷研究所實驗室完成.實驗動物為健康清潔級雄性Wistar大鼠25隻.以AA大鼠為模型,收集滑膜細胞,依次作如下分組:正常對照組,AA組,AA+青籐堿30 mg/L組,AA+青籐堿60 mg/L組,AA+青籐堿120 mg/L組.電泳遷移率改變分析法測NF-κB的活性,反轉錄PCR檢測TNF-α mRNA,IL-1β mRNA,IL-10 mRNA的錶達.主要觀察指標:不同劑量的青籐堿體外作用對後佐劑性關節炎大鼠滑膜細胞NF-κB活性,TNF-1β mRNA,IL-1β mRNA及IL-10 mRNA的對比結果.結果:與正常對照組相比,AA後滑膜細胞內NF-κB活性與TNF-α mR-NA,IL-1β mRNA,IL-10 mRNA的錶達均顯著升高,分彆為17±6,0.570±0.047,0.730±0.093,0.683±0.081(t=2.71～4.07,P<0.05).經青籐堿作用後,NF-κB活性的變化趨勢與TNF-α mRNA,IL-1 mRNA的相關性較好(r=0.810,P<0.001;r=0.562,P<0.05),與IL-10 mRNA無統計學上的相關性,青籐堿在一定的濃度範圍(30～120 mg/L)內呈濃度依賴性抑製NF-κB活性與TNF-α mR-NA,IL-1β mRNA的錶達,而對IL-10 mRNA的抑製效應與濃度無關.結論:青籐堿可能通過抑製NF-κB活性而降低滑膜細胞內TNF-α mR-NA及IL-1β mRNA的錶達.
배경:청등감시종중약청풍등중제취적생물감단체,재치료류풍습관절염(rheumatoid arthritis,RA)방면,구유교명학적료효,단기치료궤제상불청초.목적:관찰불동제량적청등감체외작용대좌제성관절염(adjuvant arthritis,AA)대서활막세포핵인자-κB(NF-κB)활성급종류배사인자(TNF-αmRNA、백세포개소1β(IL-1β)mRNA、백세포개소10(IL-10)mRNA적영향,이천명해약치료RA적가능궤제.설계:이세포위연구대상,분조대조적중복측량설계.단위:일소군의대학의원적중의과화소상연구소.재료:실험우2002-03/2002-10재전군소상연구소실험실완성.실험동물위건강청길급웅성Wistar대서25지.이AA대서위모형,수집활막세포,의차작여하분조:정상대조조,AA조,AA+청등감30 mg/L조,AA+청등감60 mg/L조,AA+청등감120 mg/L조.전영천이솔개변분석법측NF-κB적활성,반전록PCR검측TNF-α mRNA,IL-1β mRNA,IL-10 mRNA적표체.주요관찰지표:불동제량적청등감체외작용대후좌제성관절염대서활막세포NF-κB활성,TNF-1β mRNA,IL-1β mRNA급IL-10 mRNA적대비결과.결과:여정상대조조상비,AA후활막세포내NF-κB활성여TNF-α mR-NA,IL-1β mRNA,IL-10 mRNA적표체균현저승고,분별위17±6,0.570±0.047,0.730±0.093,0.683±0.081(t=2.71～4.07,P<0.05).경청등감작용후,NF-κB활성적변화추세여TNF-α mRNA,IL-1 mRNA적상관성교호(r=0.810,P<0.001;r=0.562,P<0.05),여IL-10 mRNA무통계학상적상관성,청등감재일정적농도범위(30～120 mg/L)내정농도의뢰성억제NF-κB활성여TNF-α mR-NA,IL-1β mRNA적표체,이대IL-10 mRNA적억제효응여농도무관.결론:청등감가능통과억제NF-κB활성이강저활막세포내TNF-α mR-NA급IL-1β mRNA적표체.
BACKGROUND: Sinomenine is an alkaloid monomer extracted from a Chinese medicinal herb sinomenium acutum stem. It is used in the therapy of the rheumatoid arthritis and has clear and definite therapeutic effects, but the therapeutic mechanism is unclear.OBJECTIVE: To observe the effect of sinomenine at different doses in vitro on the activity of nuclear factor-κB(NF-κβ) and mRNA expressions of the tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) andinterleukin-10 (IL-10) in the synovial cells of the rats with adjuvant arthritis(AA) to explore the probable mechanism of sinomenine in the treatment of rheumatoid arthritis(RA).DESIGN: A controlled repeated measuring study based on the cells.SETTING: Department of traditional chinese medicine and the institute of burn research of a military medical university.MATERIALS: This study was finished at the Laboratory of the Institute of Burn Research of Chinese PLA. The experimental animals were 25 healthy male Wistar rats of clean grade. The AA model rats were made and the synovial cells were collected and grouped as follows: normal control group, AA group,AA + sinomenine 30 mg/L group, AA + sinomenine 60 mg/L group, AA + sinomenine 120 mg/L group. The activity of the NF-κB was measured by the electrophoresis mobility shift assay(EMSA) . The mRNA expressions of the TNF-α, IL-1β and IL-10 were measured by reverse transcription-PCR assay.MAIN OUTCOME MEASURES: The results of the changes of the activity of the NF-κB and the mRNA expressions of the TNF-α, IL-1β and IL-10 in the synovial cells of the rats with adjuvant arthritis after the treatment with sinomenine at different doses.RESULTS: Compared with the normal control group, the activity of the NF-κB and the mRNA expressions of the TNF-α, IL-1β and IL-10 in the synovial cells in the AA group all increased significantly and the outcomes were 17±6, 0.570±0.047, 0.730±0.093, 0. 683 ±0.081 (t= 2.71 -4.07, P < 0.05). After the administration of sinomenine, the activity of NF-κB showed a good correlation with mRNA expressions of the TNF-αandIL-13(r=0.810, P <0.001; r=0.562, P <0.05), but no statistical relevance with mRNA expression of IL-10 was established. Sinomenine showed a dose-dependent inhibition on the activity of the NF-κB and the mRNA expressions of the TNF-α and IL-1β in a certain range of concentrations(30-120 mg/L), but no dose-dependent inhibition on mRNA expression of the IL-10 was observed.CONCLUSION: Through the inhibition of the activity of the NF-κB,sinomenine decreased the mRNA expressions of the TNF-α and the IL-1β in the synovial membrane cells.
