CAJ | 학술논문

已知痛觉传递调制途径之一是通过背根神经节中的小型神经元的突触前代谢型GABAB受体介导.为了探讨肽能和非肽能2个亚群的小型背根神经节(DRG)神经元是否在痛觉调制过程中发挥同等作用,本实验用免疫荧光组织化学法和激光共聚焦显微镜技术观察了DRG内肽能和非肽能2个亚群的小型神经元中GABAB受体的表达.结果显示:92%的肽能和90%的非肽能亚群的小型神经元均表达GABAB受体,这些受体存在于2个亚群的胞体及其分布在脊髓背角特定板层的中枢突中.该结果表明在痛觉调制过程中,肽能和非肽能2个亚群的小型DRG内神经元在脊髓水平发挥类似作用,但作用于脊髓背角的不同板层.
이지통각전체조제도경지일시통과배근신경절중적소형신경원적돌촉전대사형GABAB수체개도.위료탐토태능화비태능2개아군적소형배근신경절(DRG)신경원시부재통각조제과정중발휘동등작용,본실험용면역형광조직화학법화격광공취초현미경기술관찰료DRG내태능화비태능2개아군적소형신경원중GABAB수체적표체.결과현시:92%적태능화90%적비태능아군적소형신경원균표체GABAB수체,저사수체존재우2개아군적포체급기분포재척수배각특정판층적중추돌중.해결과표명재통각조제과정중,태능화비태능2개아군적소형DRG내신경원재척수수평발휘유사작용,단작용우척수배각적불동판층.
It has been reported that the small type of neurons in the dorsal root ganglion (DRG) play an important role in pain regulation by a presynaptic mechanism via the metabotropic type-B γ-aminobutyric acid receptors ( GABABR ). In order to understand whether the 2populations of the small type of the neurons, peptidergic and nonpeptidergic, in DRG share the same role, immunoflourescent histochemical methods and confocal laser scanning microscope were employed to investigate the expression of the GABABR in the peptidergic and nonpeptidergic small DRG neurons. The results revealed that 92% of the peptidergic and 90% of nonpeptidergic small DRG neurons express GABABR in their perikarya and central processes, which distribute in the various laminae of the spinal dorsal horn. These results suggest both the peptidergic and nonpeptidergic populations of the small neurons in the DRG share similar role in pain modulation via presynaptic mechanisms but in given laminae of the spinal dorsal horn.