CAJ | 학술논문

以稻草秸秆为原料,弱碱性预处理后进行酶解糖化,对预处理前后的稻草秸秆进行扫描电镜观察,研究预处理条件对稻草秸秆半纤维素、纤维素、木质素含量及损失率的影响,通过酶解还原糖的释放量来判断预处理的效果.结果表明:碱性预处理降低了稻草秸秆中木质素的含量,提高了纤维素的含量,增加了纤维素酶与底物的酶解可及度,促进了稻草秸秆酶解糖化.经2.0%NaOH、60 ℃、固液比1﹕12处理24 h后的稻草秸秆,在pH5.0、加酶量31.2 mg/g、45 ℃条件下酶解120 h的还原糖达到了790.3 mg/g,糖化率为81.01%.扫描电镜观察显示,经碱性预处理过的稻草秸秆孔隙度增大,机械组织暴露,酶解的有效比表面积增大,酶解速率加快.
이도초갈간위원료,약감성예처리후진행매해당화,대예처리전후적도초갈간진행소묘전경관찰,연구예처리조건대도초갈간반섬유소、섬유소、목질소함량급손실솔적영향,통과매해환원당적석방량래판단예처리적효과.결과표명:감성예처리강저료도초갈간중목질소적함량,제고료섬유소적함량,증가료섬유소매여저물적매해가급도,촉진료도초갈간매해당화.경2.0%NaOH、60 ℃、고액비1﹕12처리24 h후적도초갈간,재pH5.0、가매량31.2 mg/g、45 ℃조건하매해120 h적환원당체도료790.3 mg/g,당화솔위81.01%.소묘전경관찰현시,경감성예처리과적도초갈간공극도증대,궤계조직폭로,매해적유효비표면적증대,매해속솔가쾌.
Mild alkaline pretreatment and enzymatic hydrolysis of rice straw were performed. The Scanning Electron Microscopic(SEM) analysis of untreated and treated rice straw was performed. The effects of the pretreatment conditions on the content and lose ratio of hemicellulose,cellulose,lignin were studied. Alkaline pretreatment was evaluated for conversion of rice straw hemicellulose and cellulose to reducing sugars. The results showed that alkaline pretreatment dropped the content of lignin but increased that of cellulose and the cellulose surface area available to cellulose and accelerated the enzymatic saccharification of pretreated rice straw. The maximum yield of reducing sugars from rice straw by alkaline pretreatment (2%NaOH,w/v;60 ℃;ratio of solid-liquid,1﹕12,w/v;24 h) and enzymatic saccharification (45 ℃,pH5.0,120 h) using 31.2 mg enzyme preparation per g of substrate was 790.3 mg per g of rice straw. The ratio of enzymatic saccharification was 81.01%. SEM showed that the porosity increased and the mechanical tissue of rice straw was exposed,thus the effective specific surface area available to enzymatic hydrolysis of rice straw increased and the ratio of enzymatic saccharification accelerated.