CAJ | 학술논문

目的研究不同波长光照对草履虫增殖的影响,克隆草履虫感光蛋白基因.方法以双小核草履虫 Paramecium aureli为研究对象,分别置于黄色光(578～592 nm)、蓝色光(446～464 nm)、红色光(620～760 nm)、白光和自然光下,每隔1 h随机抽样法显微观察并计数;RT-PCR克隆草履虫感光蛋白基因.结果不同波长光的照射下,与自然光比较,第1天黄光组草履虫增殖显著上升(P<0.01),蓝光组和红光组草履虫增殖受到不同程度的抑制(P<0.01, P<0.05),白光组无明显差异(P>0.05);第2天,蓝光组、红光组和白光组草履虫增殖仍受到抑制(P<0.01),黄光组作用不显著(P>0.05).黄光组和白光组草履虫总RNA作为模板,克隆出大小约500 bp的rhodopsin-like基因cDNA片段, 5个不同光照组均克隆出大小约195 bp的Long wave sensitive opsin-like 基因cDNA片段.结论黄色光显著地促进草履虫增殖,蓝色光和红色光抑制其增殖;黄光和白光能诱导草履虫rhodopsin-like基因表达;Long wave sensitive opsin-like 基因在草履虫有表达.
목적연구불동파장광조대초리충증식적영향,극륭초리충감광단백기인.방법이쌍소핵초리충 Paramecium aureli위연구대상,분별치우황색광(578～592 nm)、람색광(446～464 nm)、홍색광(620～760 nm)、백광화자연광하,매격1 h수궤추양법현미관찰병계수;RT-PCR극륭초리충감광단백기인.결과불동파장광적조사하,여자연광비교,제1천황광조초리충증식현저상승(P<0.01),람광조화홍광조초리충증식수도불동정도적억제(P<0.01, P<0.05),백광조무명현차이(P>0.05);제2천,람광조、홍광조화백광조초리충증식잉수도억제(P<0.01),황광조작용불현저(P>0.05).황광조화백광조초리충총RNA작위모판,극륭출대소약500 bp적rhodopsin-like기인cDNA편단, 5개불동광조조균극륭출대소약195 bp적Long wave sensitive opsin-like 기인cDNA편단.결론황색광현저지촉진초리충증식,람색광화홍색광억제기증식;황광화백광능유도초리충rhodopsin-like기인표체;Long wave sensitive opsin-like 기인재초리충유표체.
Objective To investigate the effect of different wavelengths of light on the reproduction rate of Paramecium aureli. To clone genes encoding possible photosensitive receptor proteins. Methods Reproduction of Paramecium aureli was studied under different illumination conditions: yellow light (wave length 578～592 nm), blue light (wave length 446～464 nm), red light (wave length 620～760 nm), white light, and shaded sunlight. Samples were extracted randomly from each group every hour and the the quantity counted under a microscope. RT-PCR was employed to clone genes encoding possible photosensitive receptor proteins. Results Under light of different wavelengths, paramecium in the yellow light group propagated fastest and showed great discrepancy compared to other groups (P<0.01), the reproduction rate in the blue and red groups were suppressed (P<0.01, P<0.05). During the second day, suppression in the blue and red groups continued (P<0.01), but yellow group showed little when discrepancy compared to other groups (P>0.05). cDNA fragments of about 500 bp were cloned from RNA templates from the yellow and white groups, cDNA fragments of long wave sensitive opsin-like gene, about 195 bp in length, were cloned from 5 different groups. Conclusions Yellow light promoted reproduction of paramecium while red and blue light suppressed reproduction; yellow and white light may up-regulate the expression of gene encoding rhodopsin-like protein in Paramecium aureli; long wave sensitive opsin-like gene might be expressed in paramecium