CAJ | 학술논문

用华北地区流行的白粉菌15号生理小种,感染强抗白粉病的栽培小麦Brock和对白粉病敏感的小麦京411,通过蛋白质组技术分析其差异蛋白.结果表明,Brock经白粉菌感染12 h后,至少有6个蛋白质斑点(43 kD/pI 6.7、43 kD/pI 6.9、43 kD/pI 7.2、28 kD/pI 5.8、26 kD/pI 5.5和26 kD/pI 6.5)表达量明显增加;感染3 d后,有5个蛋白质斑点(48 kD/pI 5.6、43 kD/pI 6.9、43 kD/p17.2、28 kD/p15.8和26 kD/p15.5)表达量增加;感染5 d后,有12个新的蛋白质斑点(16 kD/pI 7.6、42 kD/pI 6.5、40 kD/pI 4.8、40 kD/pI 4.6、31 kD/pI 5.7、16 kD/pI 4.6、20 kD/pI 8.3、50 kD/pI6.7、48 kD/pI 6.6、28 kD/pI 5.7、23 kD/pI 4.8和25 kD/pI 4.7)被诱导合成,2种蛋白质斑点(26 kD/pI 4.6和17 kD/pI 7.9)消失.京411经白粉菌感染12 h后,3个蛋白质斑点(21 kD/pI 6.4、18 kD/pI 5.4和14 kD/pI 7.0)表达量增加;感染3 d后,有2个蛋白质斑点(80 kD/pI 5.4和14 kD/pI 7.0)表达量增加,1个蛋白质斑点(16 kD/pI 5.4)表达量下降;感染5 d后,有3个蛋白质斑点(50 kD/pI 7.3、40 kD/pI 7.3和24 kD/pI 7.2)表达量增加,2个斑点(40 kD/pI 4.8和14 kD/pI 7.2)表达量下降,但没有发现新的蛋白质合成.对Brock中诱导产生的12个新蛋白质斑点,利用MALDI-TOF-MS方法,于NCBI进行数据查询,其中有6个分别属于F-box亮氨酸高度重复蛋白、重金属转运/解毒蛋白、β-1,3-葡聚糖酶(两个同工体)、β-1,3-葡聚糖酶前体、锌指蛋白.功能查询表明,上述6个蛋白参与细胞周期调控、发育、激素响应、基因转录和病害防御等.推测Brock和京411感染白粉菌后,出现的蛋白质组变化可能与各自的抗、感白粉病特性有关.
용화북지구류행적백분균15호생리소충,감염강항백분병적재배소맥Brock화대백분병민감적소맥경411,통과단백질조기술분석기차이단백.결과표명,Brock경백분균감염12 h후,지소유6개단백질반점(43 kD/pI 6.7、43 kD/pI 6.9、43 kD/pI 7.2、28 kD/pI 5.8、26 kD/pI 5.5화26 kD/pI 6.5)표체량명현증가;감염3 d후,유5개단백질반점(48 kD/pI 5.6、43 kD/pI 6.9、43 kD/p17.2、28 kD/p15.8화26 kD/p15.5)표체량증가;감염5 d후,유12개신적단백질반점(16 kD/pI 7.6、42 kD/pI 6.5、40 kD/pI 4.8、40 kD/pI 4.6、31 kD/pI 5.7、16 kD/pI 4.6、20 kD/pI 8.3、50 kD/pI6.7、48 kD/pI 6.6、28 kD/pI 5.7、23 kD/pI 4.8화25 kD/pI 4.7)피유도합성,2충단백질반점(26 kD/pI 4.6화17 kD/pI 7.9)소실.경411경백분균감염12 h후,3개단백질반점(21 kD/pI 6.4、18 kD/pI 5.4화14 kD/pI 7.0)표체량증가;감염3 d후,유2개단백질반점(80 kD/pI 5.4화14 kD/pI 7.0)표체량증가,1개단백질반점(16 kD/pI 5.4)표체량하강;감염5 d후,유3개단백질반점(50 kD/pI 7.3、40 kD/pI 7.3화24 kD/pI 7.2)표체량증가,2개반점(40 kD/pI 4.8화14 kD/pI 7.2)표체량하강,단몰유발현신적단백질합성.대Brock중유도산생적12개신단백질반점,이용MALDI-TOF-MS방법,우NCBI진행수거사순,기중유6개분별속우F-box량안산고도중복단백、중금속전운/해독단백、β-1,3-포취당매(량개동공체)、β-1,3-포취당매전체、자지단백.공능사순표명,상술6개단백삼여세포주기조공、발육、격소향응、기인전록화병해방어등.추측Brock화경411감염백분균후,출현적단백질조변화가능여각자적항、감백분병특성유관.
The protein of resistant wheat (Triticum aestivum L.) variety Brock and susceptible variety Jing 411 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis (2-DE) at 12 h, 3 d, and 5 d after inoculating prevalent race No. 15 of B. graminis f. sp. tritici. In Brock, compared with control with no pathogen inoculation, at least six pro-tein spots of 43 kD/pI 6.7, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8, 26 kD/pI 5.5 and 26 kD/pI 6.5 obviously increased in content at 12 h time point; and five protein spots of 48 kD/pI 5.6, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8 and 26 kD/pI 5.5 increased in content at 3 d time point. At 5d after inoculation, 12 novel proteins were induced, viz. 16 kD/pI 7.6, 42 kD/pI 6.5, 40 kD/pI 4.8, 40 kD/pI 4.6, 31 kD/pI 5.7, 16 kD/pI 4.6, 20 kD/pI 8.3, 50 kD/pI 6.7, 48 kD/pI 6.6, 28 kD/pI 5.7, 23 kD/pI 4.8 and 25 kD/pI 4.7; simultaneously, two protein spots that were observed earlier disappeared. In Jing 411, three protein spots of 21 kD/pI 6.4, 18 kD/pI 5.4, and 14 kD/pI 7.0 increased in content at 12 h after inoculation. At the 3 d time point, two protein spots of 80 kD/pI 5.4 and 14 kD/pI 7.0 increased in content, however, one protein spot (16 kD/pI 5.4) showed decrease in protein abundance. At the 5 d time point, three protein spots of 50 kD/pI 7.3, 40 kD/pI 7.3, and 24 kD/pI 7.2 and two protein spots of 40 kD/pI 4.8 and 14 kD/pI 7.2 showed increase and decrease in protein abundance, respectively, but there were no novel protein spots induced. Among the 12 novel protein spots induced in Brock, six spots were identified using MALDI-TOF-MS and NCB1 database searching, which were F-box and leucine-rich repeat protein, heavy metal transport/detoxification protein, endo-beta-l,3-glucanase (two isozymes), beta-1,3-glucanase precursor and zinc finger protein. These proteins are involved in a wide range of physiological processes, such as cell cycle control, development, phytohormone response, and resistance to fungal disease. Thus, the proteorne changes in Brock and Jing 411 leaves are probably associated with the resistance and susceptibility to powder mildew, respectively.