中国油脂
中國油脂
중국유지
CHINA OILS AND FATS
2009年
12期
22-26
,共5页
物理法%高温大豆粕%大豆蛋白%相对分子质量%粒度%热性质
物理法%高溫大豆粕%大豆蛋白%相對分子質量%粒度%熱性質
물리법%고온대두박%대두단백%상대분자질량%립도%열성질
physical method%high temperature denatured soy meal%soy protein%relative molecular weight%size%thermal character
比较了加热、均质、超声和微波4种物理手段对提取高温大豆粕中大豆蛋白的辅助作用,并对不同方法提取的蛋白进行了聚丙烯酰胺凝胶电泳(SDS-PAGE)、粒度分布、相对分子质量分布及热性质分析.研究发现120 ℃(0.1 MPa,20 min)热处理对大豆蛋白的提取效果最好,蛋白浸出率可达65.58%.SDS-PAGE、凝胶渗透色谱(GPC)和粒度分布结果显示,加热、超声和微波处理所得蛋白提取物为高相对分子质量聚集体,但除微波处理外,其分子粒径也有所减小;均质可使蛋白提取物的相对分子质量、粒径减小.差示量热扫描(DSC)结果表明,均质处理所得提取物尚有吸热峰存在,而其他手段处理所得提取物已完全变性.
比較瞭加熱、均質、超聲和微波4種物理手段對提取高溫大豆粕中大豆蛋白的輔助作用,併對不同方法提取的蛋白進行瞭聚丙烯酰胺凝膠電泳(SDS-PAGE)、粒度分佈、相對分子質量分佈及熱性質分析.研究髮現120 ℃(0.1 MPa,20 min)熱處理對大豆蛋白的提取效果最好,蛋白浸齣率可達65.58%.SDS-PAGE、凝膠滲透色譜(GPC)和粒度分佈結果顯示,加熱、超聲和微波處理所得蛋白提取物為高相對分子質量聚集體,但除微波處理外,其分子粒徑也有所減小;均質可使蛋白提取物的相對分子質量、粒徑減小.差示量熱掃描(DSC)結果錶明,均質處理所得提取物尚有吸熱峰存在,而其他手段處理所得提取物已完全變性.
비교료가열、균질、초성화미파4충물리수단대제취고온대두박중대두단백적보조작용,병대불동방법제취적단백진행료취병희선알응효전영(SDS-PAGE)、립도분포、상대분자질량분포급열성질분석.연구발현120 ℃(0.1 MPa,20 min)열처리대대두단백적제취효과최호,단백침출솔가체65.58%.SDS-PAGE、응효삼투색보(GPC)화립도분포결과현시,가열、초성화미파처리소득단백제취물위고상대분자질량취집체,단제미파처리외,기분자립경야유소감소;균질가사단백제취물적상대분자질량、립경감소.차시량열소묘(DSC)결과표명,균질처리소득제취물상유흡열봉존재,이기타수단처리소득제취물이완전변성.
Four physical methods(heat treatment,homogenizing,ultrasonic treatment,microwave treatment) were compared on extracting protein from high temperature denatured soy meal. The protein extracts were analyzed by SDS-PAGE, size distribution, gel permeation chromatography(GPC), differential scanning calorimetry(DSC).The results showed that heating for 20 min at 121 ℃(0.1 MPa) was the best extracting method,which gave a 65.58% extracting rate of soy protein. According to SDS-PAGE and GPC analysis,protein extracts obtained by heat treatment,ultrasonic treatment and microwave treatment had larger relative molecular weight,while homogenizing treatment decreased its relative molecular weight.According to size analysis,the molecular size of protein extracts decreased except the extract obtained by microwave treatment.The results of DSC showed that there was still endothermic peak for the protein extract obtained by homogenizing treatment,while protein extracts obtained by other treatments were completely denatured.
