CAJ | 학술논문

从河北秦皇岛采集的用无水乙醇保存的鸭嘴舌形贝(Lingula anatina)样品中提取了DNA,经PCR扩增后进行克隆,得到了2条长度为682 bp的线粒体细胞色素C氧化酶第I亚基(COI)基因片段.调用GenBank中日本有明海和中国香港的鸭嘴舌形贝同源序列,用邻接法和贝叶斯法构建了系统发育树,结果表明:秦皇岛地区的鸭嘴舌形贝与日本有明海的鸭嘴舌形贝形成姊妹群,具有较近的亲缘关系,与香港的鸭嘴舌形贝亲缘关系较远.初步分析认为西太平洋地区鸭嘴舌形贝现有的地理分布并不是由浮游幼虫的扩散引起的,不同地区鸭嘴舌形贝的差异可能跟地理隔离有关.
종하북진황도채집적용무수을순보존적압취설형패(Lingula anatina)양품중제취료DNA,경PCR확증후진행극륭,득도료2조장도위682 bp적선립체세포색소C양화매제I아기(COI)기인편단.조용GenBank중일본유명해화중국향항적압취설형패동원서렬,용린접법화패협사법구건료계통발육수,결과표명:진황도지구적압취설형패여일본유명해적압취설형패형성자매군,구유교근적친연관계,여향항적압취설형패친연관계교원.초보분석인위서태평양지구압취설형패현유적지리분포병불시유부유유충적확산인기적,불동지구압취설형패적차이가능근지리격리유관.
The mitochondrial cytochrome oxidase subunit I (COI) gene fragments (682 bp) were amplified and cloned from two pure alcohol preserved Lingula anatina specimens collected from Qinhuangdao, northern China. Homologous sequences of individuals from two other northwestern Pacific locations, Hong Kong (southern China) and Ariake Sea (southern Japan), were drawn from GenBank for phylogenetic analysis. Sequences of individuals from Qinhuangdao and Ariake Sea were grouped together in the phylogenetic tree, suggesting a close relationship between these two populations. With the geographical information combined, it is suggested that it should be the geographic isolation rather than the larval dispersal which has caused the formation of the geographical pattern of L.anatina currently.