郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERISTY(MEDICAL SCIENCES)
2010年
6期
977-980
,共4页
史晓飞%赵占正%刘升云%高冠民
史曉飛%趙佔正%劉升雲%高冠民
사효비%조점정%류승운%고관민
Toll样受体9%狼疮肾炎%CpG-DNA%系统性红斑狼疮
Toll樣受體9%狼瘡腎炎%CpG-DNA%繫統性紅斑狼瘡
Toll양수체9%랑창신염%CpG-DNA%계통성홍반랑창
Toll-like receptor 9%lupus nephritis%CpG-DNA%systemic lupus erythematosus
目的：探讨Toll样受体9（TLR9）在狼疮肾炎（LN）患者肾组织的表达及其临床意义。方法：选取57例LN患者（病理分型：Ⅱ型8例,Ⅲ型12例,Ⅳ型20例,Ⅴ型17例）和11例正常对照的肾组织,应用免疫组织化学SP法检测TLR9表达情况,并对LN患者肾组织TLR9与肾脏病理和临床实验室指标相关性进行分析。结果：各型LN患者肾组织中肾小球、肾小管间质TLR9表达与正常肾组织比较差异均有统计学意义（F=19.016和17.731,P〈0.001）。LN患者肾小球TLR9表达水平与肾脏病理活动指数（AI）、肾小球细胞增殖程度、24h尿蛋白定量呈正相关（r/rs=0.891、0.563和0.417,P均〈0.001）,与肾小球滤过率（eGFR）呈负相关（r=-0.525,P〈0.001）;肾小管间质TLR9表达水平与AI、肾小管间质损害程度、24h尿蛋白定量呈正相关（r/rs=0.726、0.609和0.354,P均〈0.001）,与eGFR呈负相关（r=-0.515,P〈0.001）。结论：LN患者肾小球和肾小管间质中TLR9表达增高,并与肾脏病理活动程度相关。
目的：探討Toll樣受體9（TLR9）在狼瘡腎炎（LN）患者腎組織的錶達及其臨床意義。方法：選取57例LN患者（病理分型：Ⅱ型8例,Ⅲ型12例,Ⅳ型20例,Ⅴ型17例）和11例正常對照的腎組織,應用免疫組織化學SP法檢測TLR9錶達情況,併對LN患者腎組織TLR9與腎髒病理和臨床實驗室指標相關性進行分析。結果：各型LN患者腎組織中腎小毬、腎小管間質TLR9錶達與正常腎組織比較差異均有統計學意義（F=19.016和17.731,P〈0.001）。LN患者腎小毬TLR9錶達水平與腎髒病理活動指數（AI）、腎小毬細胞增殖程度、24h尿蛋白定量呈正相關（r/rs=0.891、0.563和0.417,P均〈0.001）,與腎小毬濾過率（eGFR）呈負相關（r=-0.525,P〈0.001）;腎小管間質TLR9錶達水平與AI、腎小管間質損害程度、24h尿蛋白定量呈正相關（r/rs=0.726、0.609和0.354,P均〈0.001）,與eGFR呈負相關（r=-0.515,P〈0.001）。結論：LN患者腎小毬和腎小管間質中TLR9錶達增高,併與腎髒病理活動程度相關。
목적：탐토Toll양수체9（TLR9）재랑창신염（LN）환자신조직적표체급기림상의의。방법：선취57례LN환자（병리분형：Ⅱ형8례,Ⅲ형12례,Ⅳ형20례,Ⅴ형17례）화11례정상대조적신조직,응용면역조직화학SP법검측TLR9표체정황,병대LN환자신조직TLR9여신장병리화림상실험실지표상관성진행분석。결과：각형LN환자신조직중신소구、신소관간질TLR9표체여정상신조직비교차이균유통계학의의（F=19.016화17.731,P〈0.001）。LN환자신소구TLR9표체수평여신장병리활동지수（AI）、신소구세포증식정도、24h뇨단백정량정정상관（r/rs=0.891、0.563화0.417,P균〈0.001）,여신소구려과솔（eGFR）정부상관（r=-0.525,P〈0.001）;신소관간질TLR9표체수평여AI、신소관간질손해정도、24h뇨단백정량정정상관（r/rs=0.726、0.609화0.354,P균〈0.001）,여eGFR정부상관（r=-0.515,P〈0.001）。결론：LN환자신소구화신소관간질중TLR9표체증고,병여신장병리활동정도상관。
Aim：To investigate the expression of Toll-like receptor 9 （TLR9） in renal tissue of patients with lupus nephritis（LN） and its clinical significance.Methods：The expression of TLR9 was examined in the renal tissue of 57 LN patients and 11 healthy controls by immunohistochemistry.The relationship between the expression of TLR9 and the renal pathological indexes and the clinical parameters was analyzed.Results：In renal tissue of LN patients,the expression of TLR9 was higher in glomerular and tubulointerstitial than in healthy controls（F=19.016 and 17.731,P0.001）. Moreover, the expression of TLR9 in glomeruli showed positive correlation with the histopathology activity index（AI） of renal tissue,glomerular cellular proliferation and 24-h urinary protein（r/rs=0.891,0.563 and 0.417,P0.001）,and negative correlation with the estimated glomerular filtration rate（eGFR）（r=-0.525,P0.001）.The expression of TLR9 in tubulointerstitial showed positive correlation with AI, the degree of tubulointerstitial damage and 24-h urinary protein（r/rs=0.726,0.609 and 0.354,P0.001）,and negative correlation with the eGFR （r=-0.515, P0.001）.Conclusion：The expression of TLR9 increases in glomerular and interstitial of LN patients,which is correlated with progress of renal histological damage.
