中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
5期
321-325
,共5页
陈志鸿%于启海%许晓群%吴荣德%刘娣%潘奇%崔明宇
陳誌鴻%于啟海%許曉群%吳榮德%劉娣%潘奇%崔明宇
진지홍%우계해%허효군%오영덕%류제%반기%최명우
肾母细胞瘤%细胞因子信号转导抑制因子-1%细胞因子信号转导抑制因子-3
腎母細胞瘤%細胞因子信號轉導抑製因子-1%細胞因子信號轉導抑製因子-3
신모세포류%세포인자신호전도억제인자-1%세포인자신호전도억제인자-3
Nephroblastoma%SOCS-1%SOCS-3
目的 探讨小儿肾母细胞瘤中细胞因子信号转导抑制因子-1(SOCS-1)和细胞因子信号转导抑制因子-3(SOCS-3)的表达,以及与小儿肾母细胞瘤不同病理类型之间的内在关系.方法 应用RT-PCR法及Western-Blot法测定10例小儿正常肾脏组织、30例小儿肾母细胞瘤及瘤旁组织中sOCS-1和SOCS-3的表达.结果 与正常小儿肾脏组织(mRNA表达:0.721±0.183、0.694±0.148,蛋白表达:0.746±0.168、0.652±0.183)相比,肾母细胞瘤组织中SOCS-1和SOCS-3 mRNA表达(0.156±0.027、0.103±0.021)及蛋白的表达(0.205±0.079、0.185±0.041)均明显降低(P＜0.01).在肿瘤不同病理类型之间SOCS-1和SOCS-3mRNA的表达(预后良好型:0.175±0.086、0.149±0.100,预后不良型:0.128±0.074、0.091±0.067)有差异(P＜0.05).瘤旁组织SOCS-1 和SOCS-3mRNA的表达(0.572±0.154、0.315±0.137)及蛋白的表达(0.628±0.183、0.439±0.137)与正常肾脏组织有差异(P＜0.05).结论 SOCS-1和SOCS-3的表达下降可能与小儿肾母细胞瘤的发生、发展密切相关,与病理分期也有一定联系.
目的 探討小兒腎母細胞瘤中細胞因子信號轉導抑製因子-1(SOCS-1)和細胞因子信號轉導抑製因子-3(SOCS-3)的錶達,以及與小兒腎母細胞瘤不同病理類型之間的內在關繫.方法 應用RT-PCR法及Western-Blot法測定10例小兒正常腎髒組織、30例小兒腎母細胞瘤及瘤徬組織中sOCS-1和SOCS-3的錶達.結果 與正常小兒腎髒組織(mRNA錶達:0.721±0.183、0.694±0.148,蛋白錶達:0.746±0.168、0.652±0.183)相比,腎母細胞瘤組織中SOCS-1和SOCS-3 mRNA錶達(0.156±0.027、0.103±0.021)及蛋白的錶達(0.205±0.079、0.185±0.041)均明顯降低(P＜0.01).在腫瘤不同病理類型之間SOCS-1和SOCS-3mRNA的錶達(預後良好型:0.175±0.086、0.149±0.100,預後不良型:0.128±0.074、0.091±0.067)有差異(P＜0.05).瘤徬組織SOCS-1 和SOCS-3mRNA的錶達(0.572±0.154、0.315±0.137)及蛋白的錶達(0.628±0.183、0.439±0.137)與正常腎髒組織有差異(P＜0.05).結論 SOCS-1和SOCS-3的錶達下降可能與小兒腎母細胞瘤的髮生、髮展密切相關,與病理分期也有一定聯繫.
목적 탐토소인신모세포류중세포인자신호전도억제인자-1(SOCS-1)화세포인자신호전도억제인자-3(SOCS-3)적표체,이급여소인신모세포류불동병리류형지간적내재관계.방법 응용RT-PCR법급Western-Blot법측정10례소인정상신장조직、30례소인신모세포류급류방조직중sOCS-1화SOCS-3적표체.결과 여정상소인신장조직(mRNA표체:0.721±0.183、0.694±0.148,단백표체:0.746±0.168、0.652±0.183)상비,신모세포류조직중SOCS-1화SOCS-3 mRNA표체(0.156±0.027、0.103±0.021)급단백적표체(0.205±0.079、0.185±0.041)균명현강저(P＜0.01).재종류불동병리류형지간SOCS-1화SOCS-3mRNA적표체(예후량호형:0.175±0.086、0.149±0.100,예후불량형:0.128±0.074、0.091±0.067)유차이(P＜0.05).류방조직SOCS-1 화SOCS-3mRNA적표체(0.572±0.154、0.315±0.137)급단백적표체(0.628±0.183、0.439±0.137)여정상신장조직유차이(P＜0.05).결론 SOCS-1화SOCS-3적표체하강가능여소인신모세포류적발생、발전밀절상관,여병리분기야유일정련계.
Objective To investigate the expression levels of suppressor of cytokine signaling-1 and suppressor of cytokine signaling-3 in the Wilms' tumor and to study the inherent relationship between the different pathological types. Methods The expression levels of SOCS-1 and SOCS-3 were assessed by RT-PCR and Western-Blot in ten normal kidney tissues, thirty nephroblastoma specimens and adjacent tissues. Results Compared to the normal kidney tissues(mRNA expressions: 0.721±0.183、0.694±0.148,protein expressions:0.746±0.168、0.652±0.183), the expressions of mRNA (0.156±0.027、0.103±0.021)and protein(0.205±0.079、0.185±0.041)of the SOCS-1 and SOCS-3 in nephroblastoma were significantly lower (P＜0.01). There were statistically significant between mRNA expressions and the different pathological types(FH:0.175±0.086,0、149±0.100,UH:0. 128±0.074、0.091±0.067) (P＜0.05).The difference betweenneuroblastoma and the normal tissues in expressions of mRNA (0. 572 ± 0. 154、0. 315 ± 0. 137)and protein(0. 628± 0. 183、0. 439 ± 0. 137)of the SOCS-1 and SOCS-3 were statistically significant (P＜0. 05). Conclusions The low SOCS-1 and SOCS-3 expressions may have a role in the development and progress of nephroblastoma, and the different pathological types.
