中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
6期
348-351
,共4页
肝炎,乙型,慢性%肝炎病毒,乙型%肝炎核心抗原,乙型%显微镜检查,共焦%基因表达
肝炎,乙型,慢性%肝炎病毒,乙型%肝炎覈心抗原,乙型%顯微鏡檢查,共焦%基因錶達
간염,을형,만성%간염병독,을형%간염핵심항원,을형%현미경검사,공초%기인표체
Hepatitis B,chronic%Hepatitis B virus%Hepatitis B core antigens%Microscopy,confocal%Gene expression
目的 探讨HBcAg在慢性HBV感染者肝细胞内的分布情况及其临床意义.方法 对41例慢性HBV感染者进行肝组织穿刺,用免疫荧光组织化学技术在激光共聚焦显微镜下观察肝细胞内HBcAg的分布情况.计量资料采用Kruskal Wallis检验,计数资料采用卡方检验.结果 41例慢性HBV感染者中,36例肝细胞内HBcAg阳性表达,阳性率为87.8%,其中23例肝功能中度异常,10例肝功能轻度异常,3例肝功能正常.HBcAg有膜、胞质与核三种形式表达.在23例肝功能中度异常者中,6例呈明显膜型表达,无明显胞质型及核型,17例以胞质型与膜型混合表达,未发现核型表达.在10例肝功能轻度异常者中,以单纯胞质型为主,未见膜型与核型表达.在3例肝功能正常者中,以胞质型表达与少许核型表达为主,未见膜型表达.HBcAg表达类型与肝功能之间差异有统计学意义(χ2=10.60,P＜0.01).结论 慢性HBV感染者肝组织损伤与HBcAg的表达有直接联系,提示膜型表达的HBcAg是肝脏免疫损伤过程中的靶抗原.
目的 探討HBcAg在慢性HBV感染者肝細胞內的分佈情況及其臨床意義.方法 對41例慢性HBV感染者進行肝組織穿刺,用免疫熒光組織化學技術在激光共聚焦顯微鏡下觀察肝細胞內HBcAg的分佈情況.計量資料採用Kruskal Wallis檢驗,計數資料採用卡方檢驗.結果 41例慢性HBV感染者中,36例肝細胞內HBcAg暘性錶達,暘性率為87.8%,其中23例肝功能中度異常,10例肝功能輕度異常,3例肝功能正常.HBcAg有膜、胞質與覈三種形式錶達.在23例肝功能中度異常者中,6例呈明顯膜型錶達,無明顯胞質型及覈型,17例以胞質型與膜型混閤錶達,未髮現覈型錶達.在10例肝功能輕度異常者中,以單純胞質型為主,未見膜型與覈型錶達.在3例肝功能正常者中,以胞質型錶達與少許覈型錶達為主,未見膜型錶達.HBcAg錶達類型與肝功能之間差異有統計學意義(χ2=10.60,P＜0.01).結論 慢性HBV感染者肝組織損傷與HBcAg的錶達有直接聯繫,提示膜型錶達的HBcAg是肝髒免疫損傷過程中的靶抗原.
목적 탐토HBcAg재만성HBV감염자간세포내적분포정황급기림상의의.방법 대41례만성HBV감염자진행간조직천자,용면역형광조직화학기술재격광공취초현미경하관찰간세포내HBcAg적분포정황.계량자료채용Kruskal Wallis검험,계수자료채용잡방검험.결과 41례만성HBV감염자중,36례간세포내HBcAg양성표체,양성솔위87.8%,기중23례간공능중도이상,10례간공능경도이상,3례간공능정상.HBcAg유막、포질여핵삼충형식표체.재23례간공능중도이상자중,6례정명현막형표체,무명현포질형급핵형,17례이포질형여막형혼합표체,미발현핵형표체.재10례간공능경도이상자중,이단순포질형위주,미견막형여핵형표체.재3례간공능정상자중,이포질형표체여소허핵형표체위주,미견막형표체.HBcAg표체류형여간공능지간차이유통계학의의(χ2=10.60,P＜0.01).결론 만성HBV감염자간조직손상여HBcAg적표체유직접련계,제시막형표체적HBcAg시간장면역손상과정중적파항원.
Objective To explore the distribution and clinical significance of hepatitis B core antigen( HBcAg) in the hepatocytes of chronic hepatitis B virus infected patients. Methods Paraffin sections were made from 41 liver biopsy samples obtained from chronic hepatitis B virus infected patients. The immuno-fluorescence confocal technique was utilized to analyze the expression level and localization of HBcAg in hepatocytes. The data were analyzed by using Kruskal Wallis test and chisquare test. Results HBcAg expression were detected in 36 (87. 8%) patients, among whom 23 cases had moderate abnormal liver function, 10 with mild abnormal liver function and 3 with normal liver function. Among the cases with moderate abnormal liver function, 6 cases showed the simple membrane-type HBcAg expression, 17 cases showed mixed cytosolic-type and membrane-type HBcAg expression without the nuclear-type expression. Twelve cases with mild abnormal liver function only showed simple cytosolic-type HBcAg expression without membrane-type or nuclear-type expression. In the three patients with normal liver function, HBcAg was expressed in cytoplasm and nuclear but not on membrane. The correlation between HBcAg expression pattern and liver function was statistically significant (χ2 =10. 60, P＜0.01). Conclusion HBcAg expression is directly correlated with liver injury in chronic hepatitis B virus infected patients, which indicates that membrane expressed HBcAg is the target antigen during the immuno-attack of liver.
