中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
10期
713-716
,共4页
王玲%李承新%朱东宁%坚哲
王玲%李承新%硃東寧%堅哲
왕령%리승신%주동저%견철
黑素细胞%酪氨酸酶相关蛋白%黑素合成%Nd:YAG激光
黑素細胞%酪氨痠酶相關蛋白%黑素閤成%Nd:YAG激光
흑소세포%락안산매상관단백%흑소합성%Nd:YAG격광
Melanocytes%Tyrosinase-related protein%Melanogenesis%Nd:YAG laser
目的 探讨Q开关Nd:YAG 1064 nm激光照射对体外培养的人表皮黑素细胞系PIG细胞黑素合成的影响.方法 选择Medlite C6 Q开关Nd:YAG激光照射体外培养的PIG细胞,MTT法检测PIG细胞的活性,多巴氧化法检测酪氨酸酶活性的变化,荧光定量反转录(RT)-PCR和Westenr印迹分别检测酪氨酸酶相关蛋白(TRP)转录和蛋白表达水平.结果 ①在不影响细胞存活率的范围内,1 J/cm2照射后24 h时,PIG细胞酪氨酸酶活性(0.563±0.014)与空白对照组(0.501±0.019)比较轻度增加(P＜0.05),酪氨酸酶与TRP-1 mRNA表达(分别为1.40±0.11,1.28±0.03)与空白对照组(1.0)比较明显上调(P＜0.05),而TRP-2 mRNA表达(0.91±0.17)并无明显变化(P＞0.05),其蛋白水平的变化与转录水平相符.②在不影响细胞活性的范围内,3 J/cm2照射后24 h时,PIG细胞酪氨酸酶活性(0.343±0.012)与空白对照组(0.501±0.019)比较明显下降(P＜0.05),酪氨酸酶、TRP-1和TRP-2的mRNA表达(分别为0.70±0.02、0.73±0.04、0.68±0.04)和蛋白表达(分别为0.64±0.05、0.86±0.17、0.69±0.11)与空白对照组(1.0)相比均不同程度的降低(P＜0.05).结论 Q开关Nd:YAG 1064 nm激光照射可以影响PIG细胞的黑素合成.在不影响细胞存活率的范围内,能量密度为1 J/cm2的Q开关Nd:YAG 1064 nm激光照射能够刺激黑素合成,而3 J/cm2的照射对黑素合成有抑制作用.
目的 探討Q開關Nd:YAG 1064 nm激光照射對體外培養的人錶皮黑素細胞繫PIG細胞黑素閤成的影響.方法 選擇Medlite C6 Q開關Nd:YAG激光照射體外培養的PIG細胞,MTT法檢測PIG細胞的活性,多巴氧化法檢測酪氨痠酶活性的變化,熒光定量反轉錄(RT)-PCR和Westenr印跡分彆檢測酪氨痠酶相關蛋白(TRP)轉錄和蛋白錶達水平.結果 ①在不影響細胞存活率的範圍內,1 J/cm2照射後24 h時,PIG細胞酪氨痠酶活性(0.563±0.014)與空白對照組(0.501±0.019)比較輕度增加(P＜0.05),酪氨痠酶與TRP-1 mRNA錶達(分彆為1.40±0.11,1.28±0.03)與空白對照組(1.0)比較明顯上調(P＜0.05),而TRP-2 mRNA錶達(0.91±0.17)併無明顯變化(P＞0.05),其蛋白水平的變化與轉錄水平相符.②在不影響細胞活性的範圍內,3 J/cm2照射後24 h時,PIG細胞酪氨痠酶活性(0.343±0.012)與空白對照組(0.501±0.019)比較明顯下降(P＜0.05),酪氨痠酶、TRP-1和TRP-2的mRNA錶達(分彆為0.70±0.02、0.73±0.04、0.68±0.04)和蛋白錶達(分彆為0.64±0.05、0.86±0.17、0.69±0.11)與空白對照組(1.0)相比均不同程度的降低(P＜0.05).結論 Q開關Nd:YAG 1064 nm激光照射可以影響PIG細胞的黑素閤成.在不影響細胞存活率的範圍內,能量密度為1 J/cm2的Q開關Nd:YAG 1064 nm激光照射能夠刺激黑素閤成,而3 J/cm2的照射對黑素閤成有抑製作用.
목적 탐토Q개관Nd:YAG 1064 nm격광조사대체외배양적인표피흑소세포계PIG세포흑소합성적영향.방법 선택Medlite C6 Q개관Nd:YAG격광조사체외배양적PIG세포,MTT법검측PIG세포적활성,다파양화법검측락안산매활성적변화,형광정량반전록(RT)-PCR화Westenr인적분별검측락안산매상관단백(TRP)전록화단백표체수평.결과 ①재불영향세포존활솔적범위내,1 J/cm2조사후24 h시,PIG세포락안산매활성(0.563±0.014)여공백대조조(0.501±0.019)비교경도증가(P＜0.05),락안산매여TRP-1 mRNA표체(분별위1.40±0.11,1.28±0.03)여공백대조조(1.0)비교명현상조(P＜0.05),이TRP-2 mRNA표체(0.91±0.17)병무명현변화(P＞0.05),기단백수평적변화여전록수평상부.②재불영향세포활성적범위내,3 J/cm2조사후24 h시,PIG세포락안산매활성(0.343±0.012)여공백대조조(0.501±0.019)비교명현하강(P＜0.05),락안산매、TRP-1화TRP-2적mRNA표체(분별위0.70±0.02、0.73±0.04、0.68±0.04)화단백표체(분별위0.64±0.05、0.86±0.17、0.69±0.11)여공백대조조(1.0)상비균불동정도적강저(P＜0.05).결론 Q개관Nd:YAG 1064 nm격광조사가이영향PIG세포적흑소합성.재불영향세포존활솔적범위내,능량밀도위1 J/cm2적Q개관Nd:YAG 1064 nm격광조사능구자격흑소합성,이3 J/cm2적조사대흑소합성유억제작용.
Objective To study to the effect of 1064-nm Q-switched Nd:YAG laser irradiation on the melanogenesis in a human epidermal melanocyte line PIG. Methods Cultured PIG cells were irradiated with 1064-nm Q-switched Nd:YAG laser (Medlite C6) at different energy densities for 10 times. After additional culture for various durations, cell viability was detected by MT assay, tyrosinase activity by dopa oxidation assay, mRNA and protein expressions of tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 by real-time quantitative fluorescent RT-PCR and Westen blotting respectively, Results The irradiation with Q-switched Nd:YAG laser at energy densities from 1 to 3 J/cm2 had no obvious effect on the viability of PIG cells. After irradiation with Nd:YAG laser at 1 J/cm2, PIG cells showed a significant increase in the tyrosinase activity,mRNA expressions of tyrosinase and TRP-1 compared with unirradiated cells (0.563 ± 0.014 vs 0.501 ±0.019, 1.40±0.11 vs 1.0, 1.28 ± 0.03 vs 1.0, all P＜ 0.05), but both the mRNA (0.91 ± 0.17 vs 1.0, P＞0.05) and protein expressions of TRP-2 experienced no significant changes before and after the irradiation.However, a significant decrease was noted in PIG cells irradiated with Nd:YAG laser at 3 J/cm2 in tyrosinase activity, mRNA and protein expressions of tyrosinase (0.70 ± 0.02 vs 1.0, 0.64 ± 0.05 vs 1.0, both P ＜ 0.05),TRP-1 (0.73±0.04 vs l.0, 0.86±0.17 vs l.0, both P＜0.05) andTRP-2 (0.68±0.04 vs l.0,0.69±0.11vs 1.0, both P ＜0.05) in comparison with unirradiated PIG cells. Conclusions The 1064-nm Q-switched Nd:YAG laser irradiation may affect the melanogenesis in PIG cells. With no influence on cell viability, the 1064-nm Q-switched Nd:YAG laser at 1 J/cm2 could enhance melanogenesis, while that at 3 J/cm2 could suppress melanogenesis, in PIG cells.
