CAJ | 학술논문

目的探讨异丙酚重复麻醉对新生大鼠海马钙离子/钙调蛋白依赖性激酶Ⅱα(CaMKⅡα)表达的影响.方法新生7d健康SD大鼠42只,体重12～16 g,雌雄各半,采用随机数字表法,将其随机分为2组(n=21):对照组(C组)和异丙酚重复麻醉组(P组).C组腹腔注射生理盐水7.5 ml/kg,连续7 d;P组腹腔注射异丙酚75 ml/kg,连续7d.出生后28 d时采用Morris水迷宫实验检测认知功能.水迷宫实验结束24h时断头处死大鼠,取脑组织,分别采用免疫组织化学法和Westernbolt法检测海马CAI区CaMKⅡα及磷酸化CaMKIⅡα(pCaMKⅡα)的表达.结果与C组比较,P组逃逸潜伏期延长,空间探索时间缩短,海马CA1区CaMKⅡα和pCaMKIIα表达下调(P＜0.01).结论异丙酚重复麻醉降低新生大鼠认知功能的机制可能与下调海马CaMKⅡα的表达和抑制其活性有关.
목적 탐토이병분중복마취대신생대서해마개리자/개조단백의뢰성격매Ⅱα(CaMKⅡα)표체적영향.방법 신생7d건강SD대서42지,체중12～16 g,자웅각반,채용수궤수자표법,장기수궤분위2조(n=21):대조조(C조)화이병분중복마취조(P조).C조복강주사생리염수7.5 ml/kg,련속7 d;P조복강주사이병분75 ml/kg,련속7d.출생후28 d시채용Morris수미궁실험검측인지공능.수미궁실험결속24h시단두처사대서,취뇌조직,분별채용면역조직화학법화Westernbolt법검측해마CAI구CaMKⅡα급린산화CaMKIⅡα(pCaMKⅡα)적표체.결과 여C조비교,P조도일잠복기연장,공간탐색시간축단,해마CA1구CaMKⅡα화pCaMKIIα표체하조(P＜0.01).결론 이병분중복마취강저신생대서인지공능적궤제가능여하조해마CaMKⅡα적표체화억제기활성유관.
Objective To investigate the effect of propofol repeated anesthesia on the expression of CaMK Ⅱ α in the hippocampus in neonatal rats.Methods Thirty-two SD rats aged 7 days weighing 12-16 g were randomly divided into 2 groups (n =16 each): group C received intraperitoneal 0.9％ normal saline 7.5 ml/kg once a day for 7 days and group P received propofol 75 mg/kg once a day for 7 days.Learning and memory function were assessed using Morris warier maze at 28 days old of rats.The animals were sacrificed at 24 h after the tests and brain tissues were removed.The expression of CaMK Ⅱ α and phosphorylated CaMK Ⅱ α (pCaMK Ⅱ α) in hippocampal CAI region were determined by immunochemistry and Western bolt.Results Compared with group C,the escape latency was significantly prolonged,space exploration time shortened and expression of CaMK Ⅱ α and pCaMK Ⅱ α down-rugulated in group P than in group C( P ＜ 0.01 ).Conclusion Propofol repeated anesthesia decreases congnitive function through down-regulating the expression and inhibiting the activity of CaMK Ⅱ α in hippocampus in neonatal rats.