CAJ | 학술논문

目的探讨脂多糖(lipopolysaccharide,LPS)预处理对非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)的影响.方法选取24只成年雄性Wistar大鼠,分为正常对照组、NASH组和LPS预处理组.NASH组饲以高糖高脂饲料;LPS预处理组饲料同肝损伤组,隔日皮下注射LPS 0.5 mg/kg;正常对照组饲以普通饲料;所有大鼠自由进食与饮水.于实验第9周末处死大鼠.制备肝组织切片,计数浸润肝组织的淋巴细胞;测定血浆内毒素水平和丙氨酸转氨酶(ALT)活性、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)含量.结果 NASH组血浆内毒素水平比正常对照组高;LPS预处理组血浆ALT水平、肝组织淋巴细胞计数与NASH组比较均显著降低;血浆TNF-α水平LPS预处理组与NASH组比较明显降低,而血浆IL-10则相反,差异有统计学意义(P＜0.05).肝组织切片HE染色结果显示,LPS预处理组与NASH组比较,肝细胞内脂肪空泡小、数量少,脂肪变性明显减轻.结论小剂量LPS预处理与减轻NASH密切相关.其机制可能是LPS预处理影响了细胞因子的释放,导致Th1/Th2细胞因子失衡,Th1向Th2偏离,可能诱导了宿主的免疫耐受,表现为肝组织损伤减轻.
목적 탐토지다당(lipopolysaccharide,LPS)예처리대비주정성지방성간염(nonalcoholic steatohepatitis,NASH)적영향.방법 선취24지성년웅성Wistar대서,분위정상대조조、NASH조화LPS예처리조.NASH조사이고당고지사료;LPS예처리조사료동간손상조,격일피하주사LPS 0.5 mg/kg;정상대조조사이보통사료;소유대서자유진식여음수.우실험제9주말처사대서.제비간조직절편,계수침윤간조직적림파세포;측정혈장내독소수평화병안산전안매(ALT)활성、종류배사인자-α(TNF-α)、백세포개소-10(IL-10)함량.결과 NASH조혈장내독소수평비정상대조조고;LPS예처리조혈장ALT수평、간조직림파세포계수여NASH조비교균현저강저;혈장TNF-α수평LPS예처리조여NASH조비교명현강저,이혈장IL-10칙상반,차이유통계학의의(P＜0.05).간조직절편HE염색결과현시,LPS예처리조여NASH조비교,간세포내지방공포소、수량소,지방변성명현감경.결론 소제량LPS예처리여감경NASH밀절상관.기궤제가능시LPS예처리영향료세포인자적석방,도치Th1/Th2세포인자실형,Th1향Th2편리,가능유도료숙주적면역내수,표현위간조직손상감경.
Objective To investigate the changes of Th1/Th2 cytokines and its relationship with lipopolysaccharide (LPS) in pretreatment of relieving nonalcoholic steatohepatitis (NASH).Methods The 24 male Wistar rats were randomly divided into 3 groups: normal control group, liver injury group and LPS pretreatment group. The rats were given normal diet in normal control group,high-sucrose and high-fat diet both in liver injury group and in LPS pretreatment group, and the rats in LPS pretreatment group were given hypodermic injection of LPS 0. 5 mg/kg every other day. The level of plasma endotoxin (ET), activity of alanine aminotransferase (ALT), content of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were determined. At the end of week 9, the rats were executed, and the liver tissue slices were prepared to investigate hepatic pathologic change by hematoxylin and eosin (HE) staining.Results The level of plasma ET was significantly higher in liver injury group than in normal control group. The level of plasma ALT and infiltrating lymphocytes in liver tissue were significantly lower in LPS pretreatment group than in liver injury group. The level of plasma TNF-α was significantly lower in LPS pretreatment group compared with liver injury group.In contrast, the level of plasma IL-10 was higher (P＜0. 05). Histology with HE staining showed that hepatocyte steatosis was obviously relieved with smaller lipid droplet in LPS pretreatment group than in liver injury group. Conclusions LPS pretreatment can alleviate high-sucrose and high-fat induced NASH. The disequilibrium of Th1/Th2 cytokines may be an important part of mechanism.