中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2009年
4期
297-300
,共4页
杜俊凯%石景森%孙学军%徐怡庄%吴瑾光
杜俊凱%石景森%孫學軍%徐怡莊%吳瑾光
두준개%석경삼%손학군%서이장%오근광
胆囊肿瘤%傅立叶变换红外光谱%细胞株
膽囊腫瘤%傅立葉變換紅外光譜%細胞株
담낭종류%부립협변환홍외광보%세포주
Gallbladder neoplasms%FT-IR%Cell line
目的 建立胆囊癌体外培养细胞株的傅立叶变换红外光谱(FT-IR spectroscopy)检测的方法.对照胆囊癌组织光谱特征,研究胆囊癌细胞株的光谱表现;为红外光谱法诊断胆囊癌奠定细胞学基础.方法 应用美国Nicolet(尼高力)公司Magna750-Ⅱ型红外光谱仪.组织标本放置于欧米采样器表面进行检测,记录红外光谱;体外培养胆囊癌细胞株(GBC-SD)采取涂于BaF2窗片的方法,进行透射红外光谱检测,得到时间轴上体外培养细胞株的光谱图;选择特征性光谱与胆囊癌组织光谱进行比对.结果 成功地获得了体外培养胆囊癌细胞株的红外光谱图;胆囊癌细胞株红外光谱特征与相应癌组织光谱特征存在异同.结论 将体外培养肿瘤细胞株在BaF2窗片上涂片后.进行FT-IR测定是行之有效的细胞红外光谱检测手段,能得到体外培养细胞的红外谱图;胆囊癌组织所表现的红外光谱特征具有胆囊癌细胞本身的红外光谱特征,同时也具有组织自身的复杂特点;FT-IR应用于胆囊癌的定性诊断有充分的细胞学基础.
目的 建立膽囊癌體外培養細胞株的傅立葉變換紅外光譜(FT-IR spectroscopy)檢測的方法.對照膽囊癌組織光譜特徵,研究膽囊癌細胞株的光譜錶現;為紅外光譜法診斷膽囊癌奠定細胞學基礎.方法 應用美國Nicolet(尼高力)公司Magna750-Ⅱ型紅外光譜儀.組織標本放置于歐米採樣器錶麵進行檢測,記錄紅外光譜;體外培養膽囊癌細胞株(GBC-SD)採取塗于BaF2窗片的方法,進行透射紅外光譜檢測,得到時間軸上體外培養細胞株的光譜圖;選擇特徵性光譜與膽囊癌組織光譜進行比對.結果 成功地穫得瞭體外培養膽囊癌細胞株的紅外光譜圖;膽囊癌細胞株紅外光譜特徵與相應癌組織光譜特徵存在異同.結論 將體外培養腫瘤細胞株在BaF2窗片上塗片後.進行FT-IR測定是行之有效的細胞紅外光譜檢測手段,能得到體外培養細胞的紅外譜圖;膽囊癌組織所錶現的紅外光譜特徵具有膽囊癌細胞本身的紅外光譜特徵,同時也具有組織自身的複雜特點;FT-IR應用于膽囊癌的定性診斷有充分的細胞學基礎.
목적 건립담낭암체외배양세포주적부립협변환홍외광보(FT-IR spectroscopy)검측적방법.대조담낭암조직광보특정,연구담낭암세포주적광보표현;위홍외광보법진단담낭암전정세포학기출.방법 응용미국Nicolet(니고력)공사Magna750-Ⅱ형홍외광보의.조직표본방치우구미채양기표면진행검측,기록홍외광보;체외배양담낭암세포주(GBC-SD)채취도우BaF2창편적방법,진행투사홍외광보검측,득도시간축상체외배양세포주적광보도;선택특정성광보여담낭암조직광보진행비대.결과 성공지획득료체외배양담낭암세포주적홍외광보도;담낭암세포주홍외광보특정여상응암조직광보특정존재이동.결론 장체외배양종류세포주재BaF2창편상도편후.진행FT-IR측정시행지유효적세포홍외광보검측수단,능득도체외배양세포적홍외보도;담낭암조직소표현적홍외광보특정구유담낭암세포본신적홍외광보특정,동시야구유조직자신적복잡특점;FT-IR응용우담낭암적정성진단유충분적세포학기출.
Objective To establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FT-IR(Fourier transform infrared)spectra of the cultured carcinoma cells with FT-IR spectroscopy,and investigating the special spectral features of the carcinoma cells comparing with the carcinoma tissues.Methods The gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna750-Ⅱ FT-1R spectrometer and corresponding FT-IR spectra was ob-tained.The cultured gallbladder carcinoma cells(GBC-SD)were centrifuged to provide a small pellet of cells for FT-IR analysis.The cell pellet was then placed on a specially designed salt plate made of BaF2.Then the infrared spectra were recorded by the same equipment.Based on previously estab-lished criteria,a comparative study was subsequently carried out between the spectra of the cultured carcinoma cells(GBC-SD)and that of the corresponding gallbladder tissues.Results Several infrared spectral features of the carcinoma cells were obtained.Conclusion The spectral features of the carci-noma cells can be well reflected from that of the carcinoma tissue,though the later iS more complicated which might originate from the intrinsic complexity of the tissue.This study SHOWS that the diagnosis of carcinoma tissue by FT-IR method exhibits sufficient cell basis.
