CAJ | 학술논문

目的对2009年山东省临沂市起无菌性脑膜炎暴发所分离的病原体进行鉴定,并分析其基因特征.方法采集无菌性脑膜炎患者脑脊液标本42份,分离病毒；RT-PCR扩增阳性分离物,测定VP1核苷酸序列,与GenBank中其他毒株进行同源性分析,并构建VPI基因亲缘进化树.结果分离到肠道病毒17株,经肠道病毒组合血清中和试验和分子分型鉴定为柯萨奇B5病毒(CVB5).CVB5分离株之间核苷酸和氨基酸同源性分别为92.3％～100.0％和98.7％～100.0％,与CVB5原型株(Faulkner株)的核苷酸和氨基酸的同源性分别为81.0％～82.4％和9 6.6％～97.0％.VP1区亲缘进化树分析显示,全球CVB5可分为A、B、C、D共4个基因型,引起本次无菌性脑膜炎暴发的CVB5属于基因型D,但位于不同的传播链中.结论 CVB5是此次临沂市无菌性脑膜炎暴发的病原体,分离株之间有较大的遗传差异,但均属于D基因型.
목적 대2009년산동성림기시기무균성뇌막염폭발소분리적병원체진행감정,병분석기기인특정.방법 채집무균성뇌막염환자뇌척액표본42빈,분리병독；RT-PCR확증양성분리물,측정VP1핵감산서렬,여GenBank중기타독주진행동원성분석,병구건VPI기인친연진화수.결과 분리도장도병독17주,경장도병독조합혈청중화시험화분자분형감정위가살기B5병독(CVB5).CVB5분리주지간핵감산화안기산동원성분별위92.3％～100.0％화98.7％～100.0％,여CVB5원형주(Faulkner주)적핵감산화안기산적동원성분별위81.0％～82.4％화9 6.6％～97.0％.VP1구친연진화수분석현시,전구CVB5가분위A、B、C、D공4개기인형,인기본차무균성뇌막염폭발적CVB5속우기인형D,단위우불동적전파련중.결론 CVB5시차차림기시무균성뇌막염폭발적병원체,분리주지간유교대적유전차이,단균속우D기인형.
Objective To identify the pathogen of an aseptic meningitis outbreak which occurred in Linyi City of Shandong Province during the summer of 2009,and to analyze the genetic variations of Coxsackicvirus B5 (CVB5) isolates.Methods Forty-two cerebrospinal fluids (CSF) specimens were collected from aseptic meningitis cases and virus isolation was performed. The viral RNA was extracted and amplified from the positive specimens using reverse transcription polymerase chain reaction (RT-PCR).The partial VP1 coding region was purified and sequenced. The phylogenetic trees based on VP1 sequences were constructed among CVB5 isolates and those in GenBank.Results Seventeen enteroviruse strains were isolated from 42 CSF samples with 40.5％ isolation positive rate. All these strains were identified as CVB5 using both microneutralization test and molecular typing methods. Homology comparisons indicated that the nucleotide acid identities and amino acid sequence identities were 92.3 ％- 100.0％ and 98.7 ％- 100.0％,respectively among these CVB5 isolate.s,and compared with the Faulkner prototype strain,which were 81.0％-82.4％ and 96.6％ 97.0％,respectively.Phylogenetic analysis on VP1 sequences showed that all CVB5 could be separated into four genogroups of A,B,C and D.Isolates of this outbreak belonged to genogroup D.Interestingly,two distinct genogroups in the phylogenetic tree were observed among the 17 isolates.Conclusions CVB5 is responsible for the outbreak of aseptic meningitis in Linyi City of Shandong Province,China. The genetic diversity is high among the isolates and all belong to genogroup D.