CAJ | 학술논문

目的探讨类固醇生成因子1(SF-1)和DAX-1在肾上腺皮质肿瘤中的表达及其临床意义.方法应用实时荧光定量PCR检测39例肾上腺皮质肿瘤:包括对血管紧张素Ⅱ无反应的醛固酮瘤(AⅡ-U-APA)12例、有反应的醛固酮瘤(AⅡ-R-APA)5例、分泌醛固酮的肾上腺皮质癌2例、皮质醇瘤10例、肾上腺无功能瘤10例及8例正常肾上腺皮质的SF-1、DAX-1、ACTH受体和内参β3-actin mRNA的表达.应用Western印迹检测SF-1和DAX-1蛋白在上述组织中的表达,比较不同组织中SF-1、DAX-1mRNA及蛋白表达的差异.对SF-1和DAX-1在肾上腺皮质肿瘤中的表达差异与患者的临床资料行相关性分析.结果 SF-1和DAX-1 mRNA及蛋白在正常肾上腺皮质、AⅡ-U-APA、AⅡ-R-APA、肾上腺皮质醛固酮癌、皮质醇瘤和无功能瘤中的表达存在差异[ SF-1 mRNA相对值:24.58±2.45、23.89±3.17、21.59±3.00、(38.75,44.16)、14.17±2.80和36.38±3.50; DAX-1 mRNA:0.57 ±0.06、0.37±0.05、0.43±0.05、(1.52,1.21)、0.39±0.04和0.83 ±0.08:SF-1蛋白:0.76±0.11、0.76±0.10、0.73±0.07、(1.24,1.40)、0.55±0.04和0.98±0.10:DAX-1蛋白:0.65±0.14、0.39±0.13、0.43±0.14、(1.18,1.02)、0.56±0.04和1.03±0.13;均P＜0.05或P＜0.01];AⅡ-U-APA组织中SF-1正常、DAX-1表达减低、SF-1/DAX-1比值增高且与醛固酮瘤的大小呈明显负相关；SF-1在皮质醇瘤中明显低表达,且与皮质醇瘤大小呈正相关；SF-1和DAX-1 mRNA及蛋白在肾上腺无功能皮质瘤中高表达、在肾上腺醛固酮分泌癌中表达有明显高于其他各组的趋势、而在嗜铬细胞瘤中无表达.结论 SF-1和DAX-1在不同肾上腺皮质肿瘤中表达各异,提示他们可能参与肾上腺皮质肿瘤的生长和激素分泌.
목적 탐토류고순생성인자1(SF-1)화DAX-1재신상선피질종류중적표체급기림상의의.방법 응용실시형광정량PCR검측39례신상선피질종류:포괄대혈관긴장소Ⅱ무반응적철고동류(AⅡ-U-APA)12례、유반응적철고동류(AⅡ-R-APA)5례、분비철고동적신상선피질암2례、피질순류10례、신상선무공능류10례급8례정상신상선피질적SF-1、DAX-1、ACTH수체화내삼β3-actin mRNA적표체.응용Western인적검측SF-1화DAX-1단백재상술조직중적표체,비교불동조직중SF-1、DAX-1mRNA급단백표체적차이.대SF-1화DAX-1재신상선피질종류중적표체차이여환자적림상자료행상관성분석.결과 SF-1화DAX-1 mRNA급단백재정상신상선피질、AⅡ-U-APA、AⅡ-R-APA、신상선피질철고동암、피질순류화무공능류중적표체존재차이[ SF-1 mRNA상대치:24.58±2.45、23.89±3.17、21.59±3.00、(38.75,44.16)、14.17±2.80화36.38±3.50; DAX-1 mRNA:0.57 ±0.06、0.37±0.05、0.43±0.05、(1.52,1.21)、0.39±0.04화0.83 ±0.08:SF-1단백:0.76±0.11、0.76±0.10、0.73±0.07、(1.24,1.40)、0.55±0.04화0.98±0.10:DAX-1단백:0.65±0.14、0.39±0.13、0.43±0.14、(1.18,1.02)、0.56±0.04화1.03±0.13;균P＜0.05혹P＜0.01];AⅡ-U-APA조직중SF-1정상、DAX-1표체감저、SF-1/DAX-1비치증고차여철고동류적대소정명현부상관；SF-1재피질순류중명현저표체,차여피질순류대소정정상관；SF-1화DAX-1 mRNA급단백재신상선무공능피질류중고표체、재신상선철고동분비암중표체유명현고우기타각조적추세、이재기락세포류중무표체.결론 SF-1화DAX-1재불동신상선피질종류중표체각이,제시타문가능삼여신상선피질종류적생장화격소분비.
Objective To detect mRNA and protein expression of steroidogenic factor-1 ( SF-1 ) and DAX-1 in human adrenocortical tumors and normal adrenal cortex,and to investigate the effect of SF-1 and DAX-1 on the steroidogenesis and development of adrenocortical tumors.Methods Total RNA and protein was extracted from angiotensin Ⅱ unresponsive aldoterone-producing adenomas ( A Ⅱ -U-APA,n =12 ),angiotensin Ⅱ responsive aldoterone-producing adenomas ( AⅡ -R-APA,n =5 ),cortisol-producing adenomas ( CPA,n =10 ),adrenal nonfunctional adenomas ( NFA,n =10 ),aldosterone-producing carcinoma ( APC,n =2 ) and normal adrenal cortex ( NAC,n =8).To analyze gene expression of SF-1,DAX-1,ACTH receptor(ACTHR),and β-actin by real-time quantitative PCR in different tissues.The protein expression of SF-1,DAX-1,and β-actin in the same tissues by Western blot.To study the relationship of ACTHR,SF-1,and DAX-1 with clinical data in adrenocortical tumors.Results The expression of SF-1,DAX-1 mRNA and protein was different in NAC,AⅡ -U-APA,A Ⅱ -R- APA,APC,CPA,and NFA tissues [ relative expression of SF-1 mRNA:24.58±2.45,23.89±3.17,21.59±3.00,(38.75,44.16),14.17±2.80,and 36.38±3.50; DAX-1 mRNA:0.57±0.06,0.37±0.05,0.43±0.05,( 1.52,1.21 ),0.39 ±0.04,and 0.83 ±0.08 ; SF-1 protein:0.76 ±0.11,0.76 ±0.10,0.73 ±0.07,(1.24,1.40),0.55±0.04,and0.98±0.10; DAX-1 protein:0.65±0.14,0.39±0.13,0.43±0.14,(1.18,1.02),0.56±0.04,and 1.03±0.13 ; all P＜0.05 or P＜0.01 ].There was negative correlation by higher SF-1/DAX-1 ratio and tumor size in AⅡ -U-APA tissues.The mRNA and protein expression of SF-1 was lower in CPA and there was the positive correlation with tumor size.Conclusion SF-1 and DAX-1 might play a key role in the development of the adrenocortical tumorigenesis and steroidogenic tissues.