中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2011年
11期
679-682
,共4页
蔡曼波%李建军%吴艳平%罗志刚
蔡曼波%李建軍%吳豔平%囉誌剛
채만파%리건군%오염평%라지강
大鼠%肾移植%抗原,CD40%T淋巴细胞,细胞毒性%融合蛋白
大鼠%腎移植%抗原,CD40%T淋巴細胞,細胞毒性%融閤蛋白
대서%신이식%항원,CD40%T림파세포,세포독성%융합단백
Rats%Kidney transplantation%Antigens,CD40%T-Lymphocytes,cytotoxic%Fusion proteins
目的 探讨细胞毒性T淋巴细胞相关抗原4融合蛋白(CTLA4Ig)基因和CD40Ig基因转染供肾对异种大鼠移植肾存活的影响.方法 以PcDNA3.1质粒为载体,通过脂质体2000将CTLA4Ig基因和CD40Ig基因转染豚鼠肾脏,再移植(异位肾移植)给SD大鼠.实验分4组进行:第1组供肾以PcDNA3.1空载体脂质体复合物转染(空载体组)；第2组供肾转染CD40Ig基因(CD40Ig转染组)；第3组供肾转染CTLA4Ig基因(CTLA4Ig转染组)；第4组供肾同时转染CTLA4Ig基因和CD40Ig基因(双基因转染组).术后观察各组血清肌酐、移植肾组织病理改变以及移植肾存活时间.结果 空载体组、CD40Ig转染组、CTLA4Ig转染组和双基因转染组受者的存活时间分别为(6.8±1.9)d、(40.7±10.9)d、(49.3±9.5)d和(75.7±8.0)d,3个转染组明显长于空载体组(P＜0.01),其中双基因转染组移植肾存活时间最长,与其他3组比较,差异均有统计学意义(P＜0.01).各组术后血清肌酐水平呈上升趋势,但升高幅度以双基因转染组为最低(P＜0.01).术后第30天,CD40Ig转染组和CTLA4Ig转染组存活大鼠的移植肾组织中可见大量淋巴细胞浸润,而双基因转染组的移植肾组织中仅见少量淋巴细胞浸润.结论 供肾局部同时转染CTLA4Ig基因和CD40Ig基因可明显延长其异种移植后的存活时间.
目的 探討細胞毒性T淋巴細胞相關抗原4融閤蛋白(CTLA4Ig)基因和CD40Ig基因轉染供腎對異種大鼠移植腎存活的影響.方法 以PcDNA3.1質粒為載體,通過脂質體2000將CTLA4Ig基因和CD40Ig基因轉染豚鼠腎髒,再移植(異位腎移植)給SD大鼠.實驗分4組進行:第1組供腎以PcDNA3.1空載體脂質體複閤物轉染(空載體組)；第2組供腎轉染CD40Ig基因(CD40Ig轉染組)；第3組供腎轉染CTLA4Ig基因(CTLA4Ig轉染組)；第4組供腎同時轉染CTLA4Ig基因和CD40Ig基因(雙基因轉染組).術後觀察各組血清肌酐、移植腎組織病理改變以及移植腎存活時間.結果 空載體組、CD40Ig轉染組、CTLA4Ig轉染組和雙基因轉染組受者的存活時間分彆為(6.8±1.9)d、(40.7±10.9)d、(49.3±9.5)d和(75.7±8.0)d,3箇轉染組明顯長于空載體組(P＜0.01),其中雙基因轉染組移植腎存活時間最長,與其他3組比較,差異均有統計學意義(P＜0.01).各組術後血清肌酐水平呈上升趨勢,但升高幅度以雙基因轉染組為最低(P＜0.01).術後第30天,CD40Ig轉染組和CTLA4Ig轉染組存活大鼠的移植腎組織中可見大量淋巴細胞浸潤,而雙基因轉染組的移植腎組織中僅見少量淋巴細胞浸潤.結論 供腎跼部同時轉染CTLA4Ig基因和CD40Ig基因可明顯延長其異種移植後的存活時間.
목적 탐토세포독성T림파세포상관항원4융합단백(CTLA4Ig)기인화CD40Ig기인전염공신대이충대서이식신존활적영향.방법 이PcDNA3.1질립위재체,통과지질체2000장CTLA4Ig기인화CD40Ig기인전염돈서신장,재이식(이위신이식)급SD대서.실험분4조진행:제1조공신이PcDNA3.1공재체지질체복합물전염(공재체조)；제2조공신전염CD40Ig기인(CD40Ig전염조)；제3조공신전염CTLA4Ig기인(CTLA4Ig전염조)；제4조공신동시전염CTLA4Ig기인화CD40Ig기인(쌍기인전염조).술후관찰각조혈청기항、이식신조직병리개변이급이식신존활시간.결과 공재체조、CD40Ig전염조、CTLA4Ig전염조화쌍기인전염조수자적존활시간분별위(6.8±1.9)d、(40.7±10.9)d、(49.3±9.5)d화(75.7±8.0)d,3개전염조명현장우공재체조(P＜0.01),기중쌍기인전염조이식신존활시간최장,여기타3조비교,차이균유통계학의의(P＜0.01).각조술후혈청기항수평정상승추세,단승고폭도이쌍기인전염조위최저(P＜0.01).술후제30천,CD40Ig전염조화CTLA4Ig전염조존활대서적이식신조직중가견대량림파세포침윤,이쌍기인전염조적이식신조직중부견소량림파세포침윤.결론 공신국부동시전염CTLA4Ig기인화CD40Ig기인가명현연장기이충이식후적존활시간.
Objective To observe the efficacy of CTLA4Ig and CD40Ig gene local cotransfection on the survival of renal allografts.Methods The kidneys of guinea pig were transfected with PcDNA 3.1+-CTLA4Ig and/or PcDNA 3.1+ -CD40Ig gene by Lipo2000,and the transfected kidneys were transplanted to SD rats.The recipients were divided into group 1 (transfected with PcDNA 3.1 +,mock group),group 2 (transfected with PcDNA 3.1 + -CD40Ig,CD40Ig group),group 3 (transfected with PcDNA3.1+ -CTLA4Ig,CTLA4Ig group) and group 4 (co-transfected with PcDNA 3.1+-CD40Ig and CTLA4Ig,CTLA4Ig+ CD40Ig group).The effects of CTLA4Ig and CD40Ig transfection were determined by Western blotting.The serum creatinine (Scr) in the recipients,the pathological changes of the allografts and the survival of renal allografts were observed.Results Significantly prolonged allografts survival time was observed in group 2 (40.7 ± 10.9 days),group 3 (49.3 ± 9.5 days) and group 4 (75.7 ± 8.0 days) as compared with group 1 (6.8 ± 1.9days),especially allografts got the longest survival time in group 4 (75.7± 8.0 days).The serum creatinine level was reduced in group 4 at 30th day as compared with that in group 2 and group 3.The lymphocytes infiltrating rate in the grafts was lowest in group 4 after transplantation.Conclusion Local co-transfection of CTLA4Ig and CD40Ig genes can prolong the survival time of renal allografts significantly.
