CAJ | 학술논문

目的观察大鼠脊髓背角中核转录因子-Kb(NF-Kb)在慢性坐骨神经挤压性损伤(CCI)疼痛模型中表达的变化规律.方法雄性SD大鼠48只,分为手术组和假手术组,每组24只,于CCI前1天、CCI后第1,4,7,14,21天各时间点测定机械痛阈及热痛阈后立即处死大鼠,每个时间点4只,取腰髓,采用Western blot方法测定NF-Kb的蛋白表达变化.结果手术组大鼠术侧机械痛阈及热痛阔明显降低,脊髓背角中NF-Kb水平表达增加,明显高十对侧和假手术组;NF-KB蛋白水平于CCI后第1天开始增加,第7天达到高峰(P<0.01),于CCI后第21天仍维持于较高水平(P<0.05).结论 CCI致大鼠脊髓背角NF-Κb活化,参与神经病理性疼痛的调控过程.
목적 관찰대서척수배각중핵전록인자-Kb(NF-Kb)재만성좌골신경제압성손상(CCI)동통모형중표체적변화규률.방법 웅성SD대서48지,분위수술조화가수술조,매조24지,우CCI전1천、CCI후제1,4,7,14,21천각시간점측정궤계통역급열통역후립즉처사대서,매개시간점4지,취요수,채용Western blot방법측정NF-Kb적단백표체변화.결과 수술조대서술측궤계통역급열통활명현강저,척수배각중NF-Kb수평표체증가,명현고십대측화가수술조;NF-KB단백수평우CCI후제1천개시증가,제7천체도고봉(P<0.01),우CCI후제21천잉유지우교고수평(P<0.05).결론 CCI치대서척수배각NF-Κb활화,삼여신경병이성동통적조공과정.
Objective To investigate the expression of nuclear factor kappa B(NF-Κb)in the lumbar spinal cord in chronic constrictive injury(CCI)of the sciatic nerve.Methods Foay-eight male Sprague-Dawley rats were divided into 2 groups(n=24 in each).The operation group received CCI of the sciatic nerve and the sham-operation group received a sham operation as a control.Their mechanical and thermal nociceptive thresholds were assessed with paw withdrawal latency (PWL) using von Frey filaments and radiant heating.Four rats were sacrificed at each time point and segments of their lumbar spinal cords were examined using Western blotting.Results Mechanical and thermal stimulation thresholds were reduced significantly after the operation.Expression of NF-Κb protein increased significantly in the ipsilateral dorsal horn of the spine compared with the contralateral side and compared with the sham-operation group.NF-Κb protein expression began to increase on the 1 st day after the operation,reached a peak at the 7th day,and stayed high throughout the experiment.Conclusion NF-Κb in the dorsal horn of the spine may be involved in neuropathie pain after CCI.