中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1613-1615
,共3页
耿文茂%张倩%苏忠学%吴亚光%秦成坤%徐健
耿文茂%張倩%囌忠學%吳亞光%秦成坤%徐健
경문무%장천%소충학%오아광%진성곤%서건
胰腺癌%T细胞%白细胞介素-2%白细胞介素-6%白细胞介素-7
胰腺癌%T細胞%白細胞介素-2%白細胞介素-6%白細胞介素-7
이선암%T세포%백세포개소-2%백세포개소-6%백세포개소-7
Pancreatic carcinoma%T cells%Interleukin-2%Interleukin-6%Interleukin-7
目的 检测60例胰腺癌患者及60例正常人外周血CD4-CD8-T细胞及白细胞介素(IL)-2、IL-6、IL-7水平,探讨其与胰腺癌的关系.方法 采用流式细胞仪检测60例胰腺癌患者外周血CD4 - CD8 -T细胞水平；酶联免疫吸附试验( ELISA)法检测60例胰腺癌患者外周血清IL-2、IL-6、IL-7水平,并与60名健康人群进行对照比较.结果 胰腺癌患者外周血CD4 - CD8 -T细胞占CD3+T细胞的比例为(3.43±0.88)％,低于正常人群(5.66±1.23)％(P＜0.05)；胰腺癌患者外周血中IL-2水平为(2.00 ±0.42) ng/L,低于正常对照组(5.50 ±0.64) ng/L(P＜0.05)；IL-6水平为(210.68±10.82) ng/L,高于正常对照组(1.77 ±0.22) ng/L(P＜0.05)；IL-7水平为(1.89±0.32) ng/L,低于正常对照组(6.35 ±0.56) ng/L(P ＜0.05).结论 胰腺癌患者外周血中CD4-CD8-T细胞的比例降低,IL-2、IL-7水平降低及IL-6水平升高可能与胰腺癌的发生有关.
目的 檢測60例胰腺癌患者及60例正常人外週血CD4-CD8-T細胞及白細胞介素(IL)-2、IL-6、IL-7水平,探討其與胰腺癌的關繫.方法 採用流式細胞儀檢測60例胰腺癌患者外週血CD4 - CD8 -T細胞水平；酶聯免疫吸附試驗( ELISA)法檢測60例胰腺癌患者外週血清IL-2、IL-6、IL-7水平,併與60名健康人群進行對照比較.結果 胰腺癌患者外週血CD4 - CD8 -T細胞佔CD3+T細胞的比例為(3.43±0.88)％,低于正常人群(5.66±1.23)％(P＜0.05)；胰腺癌患者外週血中IL-2水平為(2.00 ±0.42) ng/L,低于正常對照組(5.50 ±0.64) ng/L(P＜0.05)；IL-6水平為(210.68±10.82) ng/L,高于正常對照組(1.77 ±0.22) ng/L(P＜0.05)；IL-7水平為(1.89±0.32) ng/L,低于正常對照組(6.35 ±0.56) ng/L(P ＜0.05).結論 胰腺癌患者外週血中CD4-CD8-T細胞的比例降低,IL-2、IL-7水平降低及IL-6水平升高可能與胰腺癌的髮生有關.
목적 검측60례이선암환자급60례정상인외주혈CD4-CD8-T세포급백세포개소(IL)-2、IL-6、IL-7수평,탐토기여이선암적관계.방법 채용류식세포의검측60례이선암환자외주혈CD4 - CD8 -T세포수평；매련면역흡부시험( ELISA)법검측60례이선암환자외주혈청IL-2、IL-6、IL-7수평,병여60명건강인군진행대조비교.결과 이선암환자외주혈CD4 - CD8 -T세포점CD3+T세포적비례위(3.43±0.88)％,저우정상인군(5.66±1.23)％(P＜0.05)；이선암환자외주혈중IL-2수평위(2.00 ±0.42) ng/L,저우정상대조조(5.50 ±0.64) ng/L(P＜0.05)；IL-6수평위(210.68±10.82) ng/L,고우정상대조조(1.77 ±0.22) ng/L(P＜0.05)；IL-7수평위(1.89±0.32) ng/L,저우정상대조조(6.35 ±0.56) ng/L(P ＜0.05).결론 이선암환자외주혈중CD4-CD8-T세포적비례강저,IL-2、IL-7수평강저급IL-6수평승고가능여이선암적발생유관.
Objective To study CD4-CD8-T cells ratio and interleukin (IL)-2,IL-6,IL-7 levels in peripheral blood of 60 patients with pancreatic cancer and their correlation with the occurrence of pancreatic cancer.Methods Flow cytometry was used to analyze the CD4-CD8- T cells ratio,and enzyme linked immunosorbent assay (ELISA) was adopted to determine the IL-2,IL-6 and IL-7 levels in the peripheral blood of 60 patients with pancreatic cancer and 60 healthy volunteers.Results The ratio of CD4 CD8 T cells in CD3 + T cells of peripheral blood from 60 patients with pancreatic cancer was (3.430.88) ％,significantly lower than in healthy volunteers (5.66 ± 1.23 ) ％ ( P ＜ 0.05 ).The IL-2 level in the peripheral blood of 60 patients with pancreatic cancer was (2.00 ± 0.42) ng/L,significantly lower than in 60 healthy volunteers (5.50 ± 0.64) ng/L ( P ＜ 0.05 ).The IL-6 level in the peripheral blood of 60 patients with pancreatic cancer was (210.68 ± 10.82) ng/L,significantly higher than in 60 healthy volunteers ( 1.77 ± 0.22) ng/L ( P ＜ 0.05 ).The IL-7 level in the peripheral blood of 60 patients with pancreatic cancer was ( 1.89 ± 0.32) ng/L,significantly lower than in 60 health volunteers (6.35 ±0.56) ng/L ( P ＜ 0.05 ).Conclusion The lower CD4 - CD8 - T cells ratio,the higher expression of IL-6 and lower expression of IL-2 and IL-7 were assumed to be closely associated with the occurrence of pancreatic cancer.
