中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
11期
993-998
,共6页
马鹏飞%邢辉%廖玲洁%陈彬%赵全壁%全宇%孙峰%杨绍敏%苏斌%陈曦%邵一鸣
馬鵬飛%邢輝%廖玲潔%陳彬%趙全壁%全宇%孫峰%楊紹敏%囌斌%陳晞%邵一鳴
마붕비%형휘%료령길%진빈%조전벽%전우%손봉%양소민%소빈%진희%소일명
HIV-1%干血斑%基因型耐药性检测%聚合酶链反应
HIV-1%榦血斑%基因型耐藥性檢測%聚閤酶鏈反應
HIV-1%간혈반%기인형내약성검측%취합매련반응
HIV-1%Dried blood spots%Drug resistance genotyping%Polymerase chain reaction
目的 通过干血斑样本与血浆、全血的HIV-1基因型耐药性检测结果比较,探讨干血斑样本应用于我国患者HIV-1耐药性检测及监测的可行性.方法 选择来自安徽(10例)、云南(13例)、湖南(6例)和新疆维吾尔自治区(10例)4省(自治区)共39例AIDS患者,这些患者感染的HIV-1流行株覆盖中国主要的流行亚型(B、CRF01 AE、CRF07_BC),同时对同一患者的血浆、全血及干血斑3种类型的样本应用实验室自建的套式PCR方法扩增HIV的pol基因区,通过美国斯坦福大学的HIV耐药数据库进行耐药程度判别并比较三者的耐药性结果.结果 干血斑、全血与血浆样本相比总扩增成功率分别为95%(37/39)、92%(36/39)及100%(39/39),各亚型样本基因序列的3种样本的一致性均高于99%,同时干血斑样本的耐药性检测结果与血浆相比一致性为86%(31/36),突变位点不一致的主要原因为混合碱基导致.结论 综合PCR扩增效果及序列分析差异等因素,干血斑样本可以反映出耐药的整体流行趋势,用于我国HIV-1患者的耐药检测及监测值得推广.
目的 通過榦血斑樣本與血漿、全血的HIV-1基因型耐藥性檢測結果比較,探討榦血斑樣本應用于我國患者HIV-1耐藥性檢測及鑑測的可行性.方法 選擇來自安徽(10例)、雲南(13例)、湖南(6例)和新疆維吾爾自治區(10例)4省(自治區)共39例AIDS患者,這些患者感染的HIV-1流行株覆蓋中國主要的流行亞型(B、CRF01 AE、CRF07_BC),同時對同一患者的血漿、全血及榦血斑3種類型的樣本應用實驗室自建的套式PCR方法擴增HIV的pol基因區,通過美國斯坦福大學的HIV耐藥數據庫進行耐藥程度判彆併比較三者的耐藥性結果.結果 榦血斑、全血與血漿樣本相比總擴增成功率分彆為95%(37/39)、92%(36/39)及100%(39/39),各亞型樣本基因序列的3種樣本的一緻性均高于99%,同時榦血斑樣本的耐藥性檢測結果與血漿相比一緻性為86%(31/36),突變位點不一緻的主要原因為混閤堿基導緻.結論 綜閤PCR擴增效果及序列分析差異等因素,榦血斑樣本可以反映齣耐藥的整體流行趨勢,用于我國HIV-1患者的耐藥檢測及鑑測值得推廣.
목적 통과간혈반양본여혈장、전혈적HIV-1기인형내약성검측결과비교,탐토간혈반양본응용우아국환자HIV-1내약성검측급감측적가행성.방법 선택래자안휘(10례)、운남(13례)、호남(6례)화신강유오이자치구(10례)4성(자치구)공39례AIDS환자,저사환자감염적HIV-1류행주복개중국주요적류행아형(B、CRF01 AE、CRF07_BC),동시대동일환자적혈장、전혈급간혈반3충류형적양본응용실험실자건적투식PCR방법확증HIV적pol기인구,통과미국사탄복대학적HIV내약수거고진행내약정도판별병비교삼자적내약성결과.결과 간혈반、전혈여혈장양본상비총확증성공솔분별위95%(37/39)、92%(36/39)급100%(39/39),각아형양본기인서렬적3충양본적일치성균고우99%,동시간혈반양본적내약성검측결과여혈장상비일치성위86%(31/36),돌변위점불일치적주요원인위혼합감기도치.결론 종합PCR확증효과급서렬분석차이등인소,간혈반양본가이반영출내약적정체류행추세,용우아국HIV-1환자적내약검측급감측치득추엄.
Objective This study aimed at exploring the feasibility of using dried blood spots (DBS) to detect HIV drug resistance genotyping in China by comparing the results of drug resistance from DBS,plasma and whole blood samples. Methods Blood samples were collected from 39 AIDS patients from Anhui (10), Yunnan ( 13 ) , Hunan (6) and Xinjiang (10) provinces and autonomous regions. The HIV strains that infected these patients covered all the major HIV-1 subtypes prevailing in China ( B,CRF01_AE,CRF07_BC). HIV drug resistance genotyping assay was performed on DBS as well as on the whole blood and plasma samples from the same patients simultaneously by using an in-house nest RT-PCR method. Drug resistance levels were determined based on Stanford University HIV drug resistance database, and the results from these three types of samples were compared. Results The percentages of successful amplification of protease and reverse transcriptase regions in the pol gene were 95% ( 37/39 ) from DBS,92% ( 36/39 ) from whole blood and 100% (39/39) from plasma samples. The sequences from the three types of samples showed more than 99% identity. 86% (31/36) of the DBS samples had the same set of drug resistance mutations as those which were detected from plasma samples. The differences probably resulted from mixed bases. Conclusions There was no major difference in detecting HIV drug resistance genotyping among DBS,plasma and whole blood samples. Therefore, DBS is useful for detextion of HIV drug resistance genotyping and is particularly valuable in developing countries like China,especially in remote rural regions.
