清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2003年
4期
445-450
,共6页
田艳梅%李丕鹏%姜晓芳%张贵友%戴尧仁
田豔梅%李丕鵬%薑曉芳%張貴友%戴堯仁
전염매%리비붕%강효방%장귀우%대요인
lithium chloride%thymocyte%apoptosis
This study investigates the effect of lithium chloride (LiCl) on mouse thymocyte apoptosis.A primary culture of mouse thymocytes was preincubated with LiCl (from 5 to 500 μmol/L) before exposure to dexamethasone (DEX), the apoptosis inducer.With 100 μmol/L of LiCl, apoptotic cell death induced by DEX was almost completely prevented as determined by flow cytometric analysis, terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and DNA laddering assay.The results show that the DEX-induced increment of caspase-3 activity in thymocytes is completely eliminated by LiCl preincubation.The results suggest that LiCl may protect Balb/c mouse thymocytes from apoptosis induced by glucocorticoid in a dose-dependent matter.
