CAJ | 학술논문

背景:巨噬细胞移动抑制因子作为肿瘤坏死因子α和白细胞介素1β的上游细胞因子,其调控作用在类风湿性关节炎发病机制中居重要地位.目的:分析血清巨噬细胞移动抑制因子与类风湿关节炎活动性的相关性.设计、时间及地点:非随机化同期对照及病例对照观察,于2005-09/2006-10在解放军白求恩国际和平医院完成.对象:选择门诊及住院类风湿性关节炎患者60例,另选同期健康者30例做对照组,其年龄、性别均与病例组匹配.方法:分析60例类风湿性关节炎患者的临床资料,根据DAS(disease activity score)积分将其分为类风湿性关节炎活动组及缓解组,并与30名正常人作对照.主要观察指标:①比较两组患者晨僵时间、关节压痛指数、关节肿胀指数、类风湿因子半定量、C-反应蛋白、血沉、血小板计数.②采用双抗体夹心ELISA法检测血清巨噬细胞移动抑制因子浓度,比较类风湿性关节炎活动组、缓解组、健康对照组血清巨噬细胞移动抑制因子、白细胞介素1β、肿瘤坏死因子α水平.③分析类风湿性关节炎活动组血清巨噬细胞移动抑制因子水平与炎性指标、临床观察指标的相关性.结果:类风湿性关节炎活动组血清巨噬细胞移动抑制因子水平显著高于缓解组及正常对照组(P < 0.05),但缓解组较正常组差异无显著性意义(P > 0.05).类风湿性关节炎活动组血清巨噬细胞移动抑制因子水平与炎性指标白细胞介素1β、肿瘤坏死因子α、血沉、C-反应蛋白、血小板计数以及关节肿胀指数和关节压痛指数均呈正相关,而与年龄、病程、晨僵时间和类风湿因子半定量则无相关性.结论:血清巨噬细胞移动抑制因子水平在类风湿性关节炎活动期显著增高,与炎性指标及关节肿痛呈正相关,提示可以作为类风湿性关节炎病情活动的血清标志物.
배경:거서세포이동억제인자작위종류배사인자α화백세포개소1β적상유세포인자,기조공작용재류풍습성관절염발병궤제중거중요지위.목적:분석혈청거서세포이동억제인자여류풍습관절염활동성적상관성.설계、시간급지점:비수궤화동기대조급병례대조관찰,우2005-09/2006-10재해방군백구은국제화평의원완성.대상:선택문진급주원류풍습성관절염환자60례,령선동기건강자30례주대조조,기년령、성별균여병례조필배.방법:분석60례류풍습성관절염환자적림상자료,근거DAS(disease activity score)적분장기분위류풍습성관절염활동조급완해조,병여30명정상인작대조.주요관찰지표:①비교량조환자신강시간、관절압통지수、관절종창지수、류풍습인자반정량、C-반응단백、혈침、혈소판계수.②채용쌍항체협심ELISA법검측혈청거서세포이동억제인자농도,비교류풍습성관절염활동조、완해조、건강대조조혈청거서세포이동억제인자、백세포개소1β、종류배사인자α수평.③분석류풍습성관절염활동조혈청거서세포이동억제인자수평여염성지표、림상관찰지표적상관성.결과:류풍습성관절염활동조혈청거서세포이동억제인자수평현저고우완해조급정상대조조(P < 0.05),단완해조교정상조차이무현저성의의(P > 0.05).류풍습성관절염활동조혈청거서세포이동억제인자수평여염성지표백세포개소1β、종류배사인자α、혈침、C-반응단백、혈소판계수이급관절종창지수화관절압통지수균정정상관,이여년령、병정、신강시간화류풍습인자반정량칙무상관성.결론:혈청거서세포이동억제인자수평재류풍습성관절염활동기현저증고,여염성지표급관절종통정정상관,제시가이작위류풍습성관절염병정활동적혈청표지물.
BACKGROUND: Macrophage migration inhibitory factor (MIF) is mainly concerned with macrophage mobilizing function, as the upper stream cytokine of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), which may have critical effect in the process of the onset of rheumatoid arthritis. OBJECTIVE: To explore the correlations between the change of serum MIF and the activity of rheumatoid arthritis (RA) disease.DESIGN, TIME AND SETTING: Non-randomized control and case study, which was carried out in the Bethune International Peace Hospital of Chinese PLA from September in 2005 to October in 2006.PARTICIPANTS: Sixty RA patients were included in this study, and other thirty healthy subjects were selected as the control group. There were significant differences in age and sex between the two groups. METHODS: Clinical data of sixty RA patients were selected by carrying out retrospective analysis, then on the basis of disease activity score (DAS) accumulated points, they were divided into active and inactive group respectively, who were contrasted with 30 health adults. MAIN OUTCOME MEASURES: ① Morning stiffness (in minutes), joint tenderness index, arthrocele index, semi-quantity rheumatoid factor (RF), C-reactive protein concentration (CRP), erythrocyte sedimentation rate (ESR), and platelet count (PLT) were recorded; ② To compare the level of serum MIF, IL-1β and TNF-α among active group, inactive group, and control group; ③ The correlation analysis was carried out among the level of serum MIF, inflammatory index and clinical observation index.RESULTS: There was significantly increased in serum MIF of patients in the active group compared to of inactive and normal groups (P < 0.05), but there were no significantly differences between inactive and control groups (P > 0.05). There were significant correlations between the serum MIF concentration and active inflammatory index of RA disease, blood sedimentationrate (ESR), C-reactive protein (CRP), platelet counting (PLT), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), swell joint index (SJI) and tenderness joint, but no significant difference was observed between the serum MIF, age, disease course, morning stiffness and rheumatoid factor (RF).CONCLUSION: The serum MIF concentration is significantly increased in patients with RA, and it may be a useful parameter for monitoring disease activity of RA.